From: Phylogenetic analysis of Shiga toxin 1 and Shiga toxin 2 genes associated with disease outbreaks
Primer Name | Nucleotide Sequence 5'-3' | Target | PCR Conditionsa | ||
---|---|---|---|---|---|
 | PCR Primers |  | Denature | Anneal | Extension |
BGR1U | TCAACGAAAAATAACTTCGCTGAATCCC | Stx1 Base 1–28 | 95°C, | 61°C, | 72°C, |
BGR1D | CAGTTAATGTGGTTGCGAAGGAATTTACC | Stx1 Base (1150–1178) | 60 sec | 60 sec | 240 sec |
BGR2U | ATGAAGTGTATATTATTTAAATGGGTACTGTG | Stx2 Base 1–32 | 95°C, | 61°C, | 72°C, |
BGR2D | TCAGTCATTATTAAACTGCACTTCAG | Stx2 Base (1201–1226) | 60 sec | 60 sec | 240 sec |
 | Sequencing Primers | Target |  |  |  |
Stx1SeqF | TGTAACCGCTGTTGTACCTGG | Stx1 Base (367–387) |  |  |  |
Stx1SeqR | TTAATACTGAATTGTCATCATCATGC | Stx1 Base 778–803 |  |  |  |
Stx2SeqF | TTGCATTAGCTTCTGTTAATGCA | Stx2 Base 1000–1022 |  |  |  |
Stx2SeqR | CATTCCGGAACGTTCCAG | Stx2 Base (433–450) |  |  |  |