Panel 1 is a digital image of an Eckhardt gel showing plasmid profiles from various R. leguminosarum strains. The contents of each lane are as follows: A, VF39SM; B, LRS39401(=VF39SM cured of pSym); C, LRS39601; D, 3841; E, T3CB; F, W14-2; G, 162Y10; H, 8002; I, 8401; and J, GF160. Panel 2 is a Southern blot, of the Eckhardt gel shown in panel 1, probed with the Pst I fragment from mcpG (Fig. 1). The lane order is identical to that of the Eckhardt gel from panel 1.