Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of cultured mycobacteria in a diagnostic laboratory

Table 3 Overview of the theoretical ARDRA profiles of the species that were newly described since the start of the evaluation period (1998), as obtained after computer aided digestion of the published sequences (see also Figures 4,5,6,7).

Species	Source of isolation	Reference	GenBank Accession Numbers	*Hha* l	*Mbo* l	*Rsa* l	*Bst* UI
M. bohemicum		[34]	AJ277284, U84502	1'	4	1	3
M. botniense	Water	[35]	AF012756	2	4	3	2
M. elephantis	Elephant	[36]	AF010747	3	1	8	3'
M. goodii	Human wounds	[37]	Y12872	10	1	(1)	2'
			Y12872^a	10	1	2	2'
M. heckeshornense	Lung disease	[38]	AF1174290	1	4	3	4
M. kubicae		[39]	AF133902	10	1	6	1'
M. murale	Walls of childrens daycare centre	[40]	Unpublished
M. septicum	Catheter related bacteraemia	[41]	AF111809	4	1	6	7
M. tuberculosis subsp. caprae	Goats in Spain	[42]	AJ131120	1	1	1	1
M. tusciae		[43]	AF058299	10	1	6	2'
M. wolinskyi	Human wounds	[37]	Y12871, Y12873	10	1	1	2'

a. After resolving the ambiguity (N) present at E. coli position 95 in the Genbank sequence Y12872. An identical base (G) is found at this position in all mycobacteria.

ISSN: 1471-2180