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Table 4 Determination of PCR efficiency in 20 spiked clinical samples. S.E.: standard error, Eff.: Efficiency, std. standard samples. For each of 20 randomly selected negative samples spiked with C. pneumoniae DNA, the slope was determined as described and the efficiency calculated from the slope. The slope of each sample was compared to the slope of the standard from the same run with a t-test and the P-values are shown. Therefore, some of the samples are compared to the same standard.

From: Determination of PCR efficiency in chelex-100 purified clinical samples and comparison of real-time quantitative PCR and conventional PCR for detection of Chlamydia pneumoniae

Sample no. Slope sample S.E. sample Eff. sample Slope std. S.E. std. Eff. std. P-value*
1 -3.064 0.215 2.12 -3.406 0.232 1.97 0.28
60 -3.190 0.273 2.06     0.55
14 -2.873 0.147 2.23 -3.266 0.264 2.02 0.19
31 -3.222 0.091 2.04 -3.623 0.340 1.89 0.25
32 -3.549 0.156 1.91     0.84
78 -3.328 0.153 2.00     0.43
84 -4.318 0.512 1.70 -3.321 0.180 2.00 0.066
90 -3.379 0.177 1.98     0.82
97 -3.503 0.291 1.93 -3.716 0.187 1.86 0.54
101 -3.266 0.085 2.02     0.028
103 -3.388 0.091 1.97     0.12
118 -3.398 0.189 1.97 -3.492 0.094 1.93 0.65
127 -3.495 0.145 1.93     0.99
131 -2.809 0.108 2.27     0.00
138 -3.545 0.183 1.91 -3.300 0.101 2.01 0.24
145 -3.396 0.134 1.97     0.28
150 -3.652 0.394 1.88     0.39
159 -3.491 0.133 1.93 -3.615 0.056 1.89 0.39
160 -2.135 0.313 2.94     0.00
171 -3.531 0.088 1.92     0.42