Table 2 Primers used for cDNA synthesis, qPCR and Two-Hybrid cloning
Primer name | Sequence 5′-3′ | Purpose |
|---|---|---|
T12VN | TTTTTTTTTTTTVN | cDNA synthesis |
tpsAF | TGAGGGCTGTTGTGAATGAGC | qPCR tpsA |
tpsAR | ACTCGGAAAGCACCAAGACACC | |
tpsBF | GTGGGCAGAATCAACGGAAAG | qPCR tpsB |
tpsBR | TGAACACTTGGATAGTTCGGCAAC | |
tpsCF | TTGCCGATGCCTGCTTGTTG | qPCR tpsC |
tpsCR | TTCGCTGGATGGAAAGTAAGACAC | |
tppAF | TTGAAGACACCGTTGGGAAGAG | qPCR tppA |
tppAR | GGAGCAAAAGATGAACTCAGGAGC | |
tppBF | TGGACACTTACCTCTGGGATGAAG | qPCR tppB |
tppBR | GCTGATGGGCATTGAGTATTTCC | |
tppCF | AAAGCCAAAGCAGCCGAATC | qPCR tppC |
tppCR | TGCCCGTTAGTATCCTCAGCAGAG | |
actF | TCGTGACCTGACGGATTACCTC | qPCR actin |
actR | TGGAAGAAGGAGCAAGAGCAGTG | |
pKT25F | ACGATTTCGAGGCGGTCAAG | Confirmation of cloned cDNA to pKT25 vector |
pKT25R | GATGTGCTGCAAGGCGATTAAG | |
pUT18CF | TGTCTTCTACGAGAACCGTGCATAC | Confirmation of cloned cDNA to pUT18C vector |
pUT18CR | CGGTGAAAACCTCTGACACATGC | |
tpsAFpst | GACTCTGCAGCCGTTTCCGACAGCATGCCTT | Cloning of tpsA cDNA |
tpsAFbam | TATCTGGATCCCGTTTCCGACAGCATGCCTT | |
tpsARkpn | TGATCGGTACCAGCTCACTGTGCCACCTGCT | |
tpsBFbam | ATCAGGATCCTCCTTTTCCAATGGCTGCCAA | Cloning of tpsB cDNA |
tpsBeco | ATCAGAATTCAGCTGCAGTCATAACATAATCA | |
tpsCFxba | AGTCTCTAGAATCCAGAATGACGAAGCGCAA | Cloning of tpsC cDNA |
tpsCReco | ACTCGAATTCCGTCCATGTCAGGGCTCAA | |
tppAxbaF | ACTGTCTAGAATCCCCCATCATGCT | Cloning of tppA cDNA |
tppAecoR | AGTAGAATTCATTAACAGAACCCTCAATAC | |
tppBxbaF | TACATCTAGATGTCGCCATGACCATCTACA | Cloning of tppB cDNA |
tppBkpnR | TTCCGGTACCCCTTTCACTCCTTATCGTGA | |
tppCFsal | TATCGTCGACCACCCCAATGACGGTCTTCAT | Cloning of tppC cDNA |
tppCRbam | CATAGGATCCTCAGTCATGGCTTTCTCCGT |