Figure 4

pcr1 ⁺ mRNA is one of the critical targets of Spo5. (A) The sporulation of the spo5(S365P) strain was detected by dark brown staining with iodine vapor when pcr1 ⁺ was overexpressed. Stained patches and DIC images of the cells are shown. Scale bar, 5 μm. (B) Sporulation defects of other spo5 mutants were also suppressed by the overexpression of pcr1 ⁺. Meiosis and sporulation were induced in cells harboring pPEP1 (vector) or pPEP3-pcr1 ⁺, and the sporulation efficiency was calculated (n > 500). Error bars indicate standard deviation. (C) Meiosis was induced in spo5∆ cells harboring plasmids containing spo5 ⁺, pcr1 ⁺, atf1 ⁺, atf21 ⁺, and atf31 ⁺, or the empty vector on SSA at 30°C for 3 days, and sporulation efficiency was calculated (n > 500). Error bars indicate standard deviation.