Figure 3From: Functional significance of nuclear export and mRNA binding of meiotic regulator Spo5 in fission yeast Nuclear export is important for the function of Spo5. (A) Spo5 fused with an NLS sequence derived from SV40 large T antigen and GFP (Spo5–NLS–GFP) accumulated in the nucleus during meiosis. Scale bar, 5 μm. (B) Addition of the NLS caused deficient sporulation. Differential interference contrast (DIC) images are shown. Scale bar, 5 μm. (C) Quantitative representation of the reduction in sporulation efficiency (light blue) and the frequency of four-spore asci (magenta) (n > 500). Error bars indicate standard deviation. (D) Nuclear accumulation of Spo5(RRM1∆ )–GFP was suppressed by the fusion of the NES sequence. Scale bar, 5 μm. (E) Addition of NES did not suppress the sporulation defects of Spo5(RRM1∆ ) (n > 500). Error bars indicate standard deviation.Back to article page