- Research article
- Open Access
A negative effect of Campylobacter capsule on bacterial interaction with an analogue of a host cell receptor
© Rubinchik et al.; licensee BioMed Central Ltd. 2014
Received: 14 November 2013
Accepted: 6 May 2014
Published: 31 May 2014
Campylobacter jejuni (C. jejuni) is the leading causative agent of bacterial gastrointestinal infections. The rise of antibiotic resistant forms of this pathogen necessitates the development of novel intervention strategies. One approach is the design of drugs preventing bacterial attachment to host cells. Although some putative C. jejuni adhesins have been identified, the molecular mechanisms of their interaction with host cells and their role in pathogenesis remain to be elucidated. C. jejuni adhesion may also be modulated by a bacterial capsule. However, the role of this structure in adhesion was not clear due to conflicting results published by different research groups. The aim of this study was to clarify the role of capsule in bacterial interaction with host cells by using an in vitro model of adhesion and an analogue of a host cell receptor.
In this study, we developed an in vitro bacterial adhesion assay, which was validated using various tests, including competitive inhibition studies, exoglycosydase treatment and site-directed mutagenesis. We demonstrate that PEB3 is one of the cell surface glycoproteins required for bacterial interaction with an analogue of a host cell receptor. In contrast, JlpA glycoprotein adhesin is not required for such interaction. We demonstrate that the production of capsule reduces bacterial attachment, and that the genes involved in capsule and PEB3 adhesin biosynthesis are differentially regulated.
In this study we report an in vitro model for the investigation of bacterial interaction with analogs of host cell receptors. The results suggest an interfering effect of capsule on bacterial attachment. In addition, using a liquid culture, we demonstrate differential expression of a gene involved in capsule production (kpsM) and a gene encoding a glycoprotein adhesin (peb3). Further studies are required in order to establish if these genes are also differentially regulated during the infection process. The results will assist in better understanding of the mechanism of pathogenesis of C. jejuni in general and the role of capsule in the process in particular.
Campylobacter jejuni (C. jejuni), a microaerophilic, spiral-shaped, flagellated Gram-negative bacterium, is the most frequent cause of human gastroenteritis worldwide . C. jejuni infections are often caused by consumption of undercooked poultry, unpasteurised milk or contaminated water . Adhesion of C. jejuni to host cells plays an important role in colonisation of chickens and in human infection . Campylobacter binding to host cell receptors is not mediated by fimbria or pili, like in E. coli and Salmonella. As noted in a recent review, other bacterial cell structures may contribute to interaction of Campylobacter with host cells .
In some cases, bacterial adhesion can be mediated by oligosaccharides present on the surface of host cells [6, 7]. In other cases, it is a pathogen oligosaccharide that is responsible for binding to specific, lectin-like, host cell structures. For example, a pathogenic Gram-positive bacterial species Nocardia rubra binds to a human lectin (intelectin) expressed by cells in different organs including intestine . There is a possibility of similar lectin-glycan interactions between host cells and Campylobacter. For example, C. jejuni adhesion to Caco-2 cell receptors was inhibited by certain lectins .
Campylobacter is capable of producing a variety of glycoproteins, some of which are cell-surface located . Inactivation of the N-linked glycosylation system reduces bacterial ability to adhere to epithelial cells and thereby colonise the gastrointestinal tract [11, 12]. These findings suggest a possible role of some bacterial cell surface surface-located bacterial N-linked glycoproteins in interaction with host cell receptors. Van Sorge and colleagues  demonstrated interaction of N-linked glycoproteins of C. jejuni with C-type lectins of Macrophage Galactose-type lectins (MGL). In similarity with other pathogens, the production of cell surface structures interacting with C-type lectins may assist C. jejuni in the evasion of the host immune response [14, 15].
Another cell surface structure that may affect bacterial interaction with host cell receptors is a capsular polysaccharide (CPS) [16–19]. Inactivation of the capsule production machinery in strain 81–176 led to a two-fold decrease in adhesion to INT407 cells . Similar findings were observed in another capsule deficient mutant, 81116/kpsE. However, these data were not supported by complementation studies. Moreover, they are in disagreement with other studies where the absence of capsule showed increased adhesion of C. jejuni strain 11168H to Caco-2 cells . The contradictory results may be a consequence of differences in assay conditions, bacterial strains and tissue cell lines.
In general, the capsules may play different roles in bacterial attachment. This depends on the nature of a bacterial pathogen, and on the structural features of the capsules and adhesins. For example, F1 capsule of a Yersinia pestis prevents fimbrial adhesins from interaction with host cell receptors , while production of a capsule by Neisseria meningitidis does not affect PilC1 adhesin-mediated bacterial attachment .
In this study we developed and evaluated an in vitro ELISA-like assay for the investigation of C. jejuni interaction with host cell receptors. The assay was successfully used to study a role of capsule in attachment using SBA (Soya bean agglutinin) lectin as an analogue of a host cell receptor. In addition, using targeted mutagenesis (supported by complementation analysis) we investigated a role of PEB3 and JlpA adhesins in this interaction. Furthermore, using real time PCR, we found that peb3 and a capsule-related gene are differentially expressed. The results of these experiments suggest an interplay between bacterial capsule and adhesins in interaction with host cells.
Dose-dependent specific binding of C. jejuni cells to immobilised SBA lectin
In order to investigate the mechanisms and factors involved in C. jejuni interaction with host cells, we developed an ELISA-like assay based on monitoring bacterial binding to immobilised analogues of host cell receptors. In this model we explored the idea that some N-link glycosylated proteins may be expressed on the bacterial cell surface, and may potentially play a role of adhesins. As glycan moieties in these glycoproteins contain terminal GalNAc residues recognised by SBA, we used the latter as an analogue of a host cell receptor.
Expression of PEB3 is required for binding of C. jejuni cells to immobilised SBA lectin
The results of this experiment also showed that peb3 mutation did not completely eliminate binding, suggesting that other glycoprotein(s) may be involved in specific interactions with this analogue of a host cell receptor. This hypothesis was supported by reduction of the residual binding of 11168H/peb3::kan r mutant in the presence of soluble lectin (Figure 5).
One of the other cell surface-located proteins of C. jejuni is JlpA, which was found to be an adhesin specifically binding to heat shock protein 90 . As JlpA was also predicted to be an N-link glycosylated protein , there was a possibility that it might be responsible for residual binding of 11168H/peb3::kan r mutant. To verify this hypothesis, we constructed a jlpA mutant and tested the effect of this mutation on attachment. Surprisingly, none of the three independent clonal isolates showed any difference when compared with the control recipient strain 11168H (data not shown) suggesting the presence of other GalNAc-containing adhesins.
Production of capsule has a negative effect on binding
The results shown in Figure 3 also have demonstrated a significantly higher efficiency of binding of the non-capsular mutant of strain 11168H. These results, confirmed by analysis of three independent clonal isolates of this mutant (data not shown), revealed significant increase in binding upon inactivation of bacterial ability to produce capsule, suggesting an interfering effect of the later on the bacterial interaction with host cell receptors.
Peb3 and capsule-related genes are differentially expressed
In this study, a model of bacterial attachment was developed. This model is based on monitoring bacterial binding to immobilized analogues of host cell receptor. Although we only tested attachment of Campylobacter jejuni to SBA lectin, the method may have wider application for investigation of interaction of other bacteria with other host cell receptors and their analogues.
The system was successfully tested by using C. jejuni strain 11168H and its isogenic mutant 11168H/peb3. Using the assay, we investigated interaction of bacteria carrying cell surface located GalNAc residues with immobilised SBA lectin. The binding was found to be specific and dependent on the presence of soluble lectin and GalNAc molecules, and was abolished by bacterial deglycosylation. The study suggests the ability of C. jejuni to produce various cell surface GalNAc-containing cell surface structures.
The SBA lectin used in this study shares binding specificity with C-type lectins (including MGL receptors) produced by host cells. According to a recent study, Campylobacter has the ability to interact with MGL receptors expressed on macrophages and dendritic cells (DCs), which may modulate host immune response . Human MGL receptors specifically recognise terminal GalNAc residues [29, 30]. Together with other C-type lectins, the MGL receptors may be recognised by viruses, e.g. a filovirus . In addition, it was shown that MGL recognizes a GalNAc containing antigen of a helminth parasite Shistosoma mansoni. Despite some data suggesting a role of MGL receptors as a host defence factor, the role of these molecules in C. jejuni infection is not clear. However, there is a possibility that, via interaction with MGL expressing macrophages and DCs this pathogen may subvert host immune response. It was suggested that C. jejuni with functional MGL ligand (GalNAc) may decrease IL-6 production by DCs .
Campylobacter have been known to produce a number of N-glycoproteins, including PEB3 . However, it was still unclear which glycoprotein is reactive with MGL. Our results demonstrated that peb3 mutation reduces but does not completely eliminate binging, suggesting the presence of other cell surface structures responsible for attachment. Surprisingly, mutation in jlpA gene, encoding another cell surface glycoproptein, had no effect on the ability of C. jejuni to bind to the immobilized SBA lectin. According to other studies, jlpA mutation also had no effect on invasion of host cells [34, 35].
An isogenic non-capsulated mutant 11168H/kpsM showed an increase in binding indicating a negative effect of capsule on bacterial attachment. In addition, results of RT-PCR showed an increase of peb3 and a decrease of kpsM gene expression over time, suggesting that a shielding effect of capsule may be essential at the initial stages of infection, hiding bacterial cell surface structures. Subsequent down regulation of CPS production during colonisation may lead to exposure of other bacterial cell surface structures required for the attachment and/or evasion of host immune response.
The results of this study demonstrated a complex interplay of Campylobacter capsule and glycoprotein adhesins in pathogen-host interaction. The developed assay will assist in more detailed investigation of such interaction and in the development of inhibitors of attachment as novel antibacterials.
Bacterial strains and growth conditions
C. jejuni strain 11168H and its isogenic mutant 11168H/kpsM::kan r were described previously [19, 36]. C. jejuni was grown under microaerophilic conditions (5% O2, 10% CO2, 85% N2) at 37°C on Columbia Blood Agar (Oxoid) containing 6% defibrinated horse blood (Fisher) and Skirrow supplement (Sigma). Antibiotics (chloramphenicol 10 μg/ml and/or kanamycin 50 μg/ml) were added to the media as required. E. coli strains XL1 and XL2 (Stratagene) were used in cloning experiments. E. coli strains were maintained on Luria–Bertani agar (Oxoid) plates or in Luria–Bertani broth (Oxoid) supplemented with appropriate antibiotics (ampicillin 100 μg/ml, kanamycin 50 μg/ml or chloramphenicol 34 μg/ml) at 37°C.
General cloning techniques
Plasmids used in this study
Source of kan r cassette
Source of cam r cassette
Contains pBAD promoter
C. jejuni 16 kb fragment, containing pgl gene cluster, cloned into pBR322
Cassette cloned into pRR (fragment of rRNA gene cluster cloned into pGEM-T easy)
Construction of C. jejuni mutants
Primers used for mutation of peb3 and jlpA and for complementation of peb3
Mutation of peb3 gene
Mutation of peb3 gene
Mutation of jlpA gene
Mutation of jlpA gene
Complementation of peb3 mutation
Complementation of peb3 mutation
Complementation of peb3 mutant
Peb3 gene was PCR amplified using primers described in Table 1. The product was digested with Xba I enzyme and cloned into Xba I-digested pRRC plasmid to produce pRRC_peb3. Restriction analysis verified that the gene was transcribed in the same orientation as the cam r gene. After transformation of the 11168H/peb3::kan r mutant with plasmid pRRC_peb3, KanrCamr clones were selected. PCR analysis confirmed integration of peb3 gene into one of the rRNA gene clusters. The complementation derivative was designated 11168H/peb3::kan r /peb3 + .
Bacterial attachment was studied in ELISA-like assay using a 96-well microtiter plate Maxisorp™ (Thermo Scientific) coated Soya Bean Agglutinin (SBA) lectin (Sigma) in bicarbonate-coating buffer: 5.3 g/L Na2CO3, 4.2 g/L NaHCO3, 1 g/L sodium azide, pH 9.6.
Microtiter plate wells were incubated overnight with SBA lectin (10 μg/ml) at 4˚C, followed by blocking with 1% Bovine Serum Albumin (BSA) overnight at 4°C. BSA-coated, wells were used as negative control. Bacteria (two-day cultures of C. jejuni or one-day cultures of E. coli) were harvested, resuspended in Phosphate-Buffered Saline (PBS) to OD600 = 1, 0.1 ml suspensions (corresponding to 4x108 c.f.u. of C. jejuni) were added to each well of the microtiter plate, followed by incubation for 40 min at room temperature. After rinses with PBS, supplemented with 0.2% Tween (PBST) the plate was incubated with biotinylated SBA lectin (Vectors Laboratories) for 60 min at room temperature. The wells were then treated with horseradish peroxidase-conjugated streptavidin (Sigma) for 30 min at room temperature followed by incubation with TMB (3,3′,5,5′-Tetramethylbenzidine) substrate (Sigma) for 10 min. The reaction was stopped by adding stop solution (1 M H2SO4). Binding was monitored by measuring OD at 450 nm. For inhibition studies, the plates were treated with bacterial suspension in the presence of SBA lectin or GalNAc (Sigma) at different concentrations. Detachment was carried out by addition to wells with immobilised bacteria of either soluble SBA lectin or GalNAc, followed by incubation for 40 min at room temperature.
Fluorescein SBA (FSBA) labelling of C. jejuni and E.coli cells
Fluorescein labelling of cells was done as described previously . FSBA (Vector Laboratories) (100 μg/ml in PBS) was mixed with an equal volume of bacterial suspension and incubated for 40 min at room temperature. Bacteria were pelleted, washed twice in PBS to remove any unbound lectin. Samples were observed by fluorescence microscopy using a laser scanning confocal microscope (Leica TCS SP2 AOBS) with a 63X immersion objective.
Treatment with exo-glycosidase
In order to remove GalNAc residues bacterial cells were treated with 20 U of N- acetylgalactosaminidase (NEB) for 60 min at 37°C according to manufacturer’s protocol.
RNA isolation and RT-PCR
For RNA isolation, C. jejuni cells were grown for 48 hours under microaerophilic conditions (5% O2, 10% CO2, 85% N2) at 37° in three separate flasks (biological replicates) in Brain Heart Infusion Broth (Oxoid). Samples for RNA isolation were taken at 14 h, 24 h, 38 h and 48 h intervals. Immediately after taking the samples from the flasks RNAprotect Bacteria Reagent (Qiagen) was added to the cultures to stabilize mRNA. The total RNA from each sample was extracted using the RNeasy Mini Kit (Qiagen). The purified RNA samples were treated with On-Column DNaseDigestion Kit (Qiagen) followed by treatments with DNase in order to remove residual DNA contamination. RNA concentration was estimated using NanoDrop ND-1000 spectrophotometer (NanoVue). The quality and integrity of total RNA was monitored using the Agilent 2100 Bioanalyzer (Agilent Technologies).
Primers used for RT-PCR
Primer sequence (5′-3′)
The cycle parameters were as follows: an initial reverse transcription step for 10 min at 50°C, and then PCR initial activation step for 5 min at 95°C followed by two-step cycling: denaturation at 95°C for 10 s and combined annealing 60°C for 30 sec. The number of cycles was 35. The changes in gene expression (n-fold) calculated from the qRT-PCR data. Analysis of relative gene expression data was done using the 2-2∆∆C T method as described previously . The 16S rRNA was used as the internal controls.
All experiments were repeated a minimum of three times, and data are expressed as mean ± SD. Differences were considered significant for P < 0.05 (*, P value 0.05-0.01; **, P value <0.01). Comparison of two groups was made with an unpaired, two-tailed student’s t-test. Comparison of multiple groups was made with ANOVA.
The study was not supported by any external funding.
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