Table 1 Bacterial strains and plasmids used in this study
Strain | Description | Reference |
|---|---|---|
Acinetobacter sp. | ||
Tol 5 | Wild type strain | [19] |
G4 | A Tol 5 mutant constructed by insertion of a FRT site in the upstream of ataA of Tol 5, Gmr, SacB | This study |
G4K1 | A Tol 5 mutant constructed by additional insertion of a FRT site in the downstream of ataA of G4, Gmr, Kmr, SacB | This study |
4140 | Unmarked ΔataA mutant of Tol 5 constructed by FLP/FRT recombination in G4K1 | This study |
E. coli | ||
DH5α | Host for routine cloning | TaKaRa |
S17-1 | Donor strain for conjugation | [4] |
Plasmid | ||
pJQ200sk | Mobile plasmid, SacB, Gmr | [32] |
pK18mob | Mobile plasmid, Kmr | [33] |
pLOI2224 | Source of FRT sites, Kmr | [34] |
pFT-A | Source of FLP recombinase and tetR, Ampr | [34] |
pJQFRT | Gene replacement vector harboring a single FRT sequence, SacB, and Gmr | This study |
pKFRT | Mobile plasmid harboring a single FRT sequence, Kmr | This study |
pKFRT/FLP | Gene replacement vector harboring a single FRT sequence, FLP recombinase under the control of Ptet promoter, and Kmr | This study |
pJQFRT_AtaAupstream | A 1.0-kb fragment containing the upstream region of ataA ligated into the BamHI site of pJQFRT | This study |
pKFRT/FLP_AtaAdownstream | A 2.8-kb fragment containing the downstream region of ataA ligated into the BamHI site of pKFRT/FLP | This study |