Table 1 Bacterial strains and plasmids used in this study

Acinetobacter sp.

Tol 5

Wild type strain

G4

A Tol 5 mutant constructed by insertion of a FRT site in the upstream of ataA of Tol 5, Gm^r, SacB

G4K1

A Tol 5 mutant constructed by additional insertion of a FRT site in the downstream of ataA of G4, Gm^r, Km^r, SacB

4140

Unmarked ΔataA mutant of Tol 5 constructed by FLP/FRT recombination in G4K1

E. coli

DH5α

Host for routine cloning

S17-1

Donor strain for conjugation

Plasmid

pJQ200sk

Mobile plasmid, SacB, Gm^r

pK18mob

Mobile plasmid, Km^r

pLOI2224

Source of FRT sites, Km^r

pFT-A

Source of FLP recombinase and tetR, Amp^r

pJQFRT

Gene replacement vector harboring a single FRT sequence, SacB, and Gm^r

pKFRT

Mobile plasmid harboring a single FRT sequence, Km^r

pKFRT/FLP

Gene replacement vector harboring a single FRT sequence, FLP recombinase under the control of Ptet promoter, and Km^r

pJQFRT_AtaAupstream

A 1.0-kb fragment containing the upstream region of ataA ligated into the BamHI site of pJQFRT

pKFRT/FLP_AtaAdownstream

A 2.8-kb fragment containing the downstream region of ataA ligated into the BamHI site of pKFRT/FLP