Glutamate 83 and arginine 85 of helix H3 bend are key residues for FtsZ polymerization, GTPase activity and cellular viability of Escherichia coli: lateral mutations affect FtsZ polymerization and E. coliviability
Glutamate 83 and arginine 85 of helix H3 bend are key residues for FtsZ polymerization, GTPase activity and cellular viability of Escherichia coli: lateral mutations affect FtsZ polymerization and E. coliviability
FtsZ is an essential cell division protein, which localizes at the middle of the bacterial cell to mediate cytokinesis. In vitro, FtsZ polymerizes and induces GTPase activity through longitudinal interactions to form the protofilaments, whilst lateral interactions result within formation of bundles. The interactions that participate in the protofilaments are similar to its eukaryotic homologue tubulin and are well characterized; however, lateral interactions between the inter protofilaments are less defined. FtsZ forms double protofilaments in vitro, though the key elements on the interface of the inter-protofilaments remain unclear as well as the structures involved in the lateral interactions in vivo and in vitro. In this study, we demonstrate that the highly conserved negative charge of glutamate 83 and the positive charge of arginine 85 located in the helix H3 bend of FtsZ are required for in vitro FtsZ lateral and longitudinal interactions, respectively and for in vivo cell division.
The effect of mutation on the widely conserved glutamate-83 and arginine-85 residues located in the helix H3 (present in most of the tubulin family) was evaluated by in vitro and in situ experiments. The morphology of the cells expressing Escherichia coli FtsZ (E83Q) mutant at 42°C formed filamented cells while those expressing FtsZ(R85Q) formed shorter filamented cells. In situ immunofluorescence experiments showed that the FtsZ(E83Q) mutant formed rings within the filamented cells whereas those formed by the FtsZ(R85Q) mutant were less defined. The expression of the mutant proteins diminished cell viability as follows: wild type > E83Q > R85Q. In vitro, both, R85Q and E83Q reduced the rate of FtsZ polymerization (WT > E83Q >> R85Q) and GTPase activity (WT > E83Q >> R85Q). R85Q protein polymerized into shorter filaments compared to WT and E83Q, with a GTPase lag period that was inversely proportional to the protein concentration. In the presence of ZipA, R85Q GTPase activity increased two fold, but no bundles were formed suggesting that lateral interactions were affected.
We found that glutamate 83 and arginine 85 located in the bend of helix H3 at the lateral face are required for the protofilament lateral interaction and also affects the inter-protofilament lateral interactions that ultimately play a role in the functional localization of the FtsZ ring at the cell division site.
Bacterial cell division occurs in the middle of the dividing cell mediated by protein complex division machinery called divisome. FtsZ (Fts, Filamentous temperature sensitive) is the principal protein of the divisome in Escherichia coli, and assembles into the Z ring at the cell division site; it is bound to the inner membrane and is stabilized by FtsA and ZipA [1, 2]. This event is necessary for the sequential addition of at least twelve other membrane proteins responsible for peptidoglycan assembly and membrane constriction . Inactivation of the corresponding genes blocks the cell division and give rise to long multinucleated cells that fail to propagate and eventually die .
The Z-ring and its dynamics during the cell process have been observed by fluorescence microscopy in living cells  and in liposomes . Although, the organization of FtsZ in the Z-ring is not known, based on the in vitro studies it is believed that the basic units to form the ring are the protofilaments. In order to explain the Z-ring dynamics at the molecular level, two theoretical models have been proposed. One model involves the hydrolysis of GTP to generate the force for invagination of the plasma membrane  and the other model proposes a microphase transition of the FtsZ filaments that is energized by lateral interactions between filaments during the constriction of the plasma membrane . In both models, the lateral interactions between the protofilaments are crucial for the membrane constriction. However, the role of lateral interactions has been questioned because the interaction entropy was not considered in the formation of the filaments . Therefore, the question regarding the role of lateral interactions still remains unclear.
Mutations on the lateral interface of FtsZ induce the formation of an aberrant Z-ring in E. coli cells and cell division can be compromised [10, 11]. Apparently, the lateral interactions to form the Z-ring are also important in B. subtilis. Recently, the Z-ring was observed in vivo at a higher resolution. The authors proposed that FtsZ polymerizes into short filaments that assemble into loose bundles mediated by longitudinal and lateral interactions to finally locate as a Z-ring at the division site . These observations suggest that lateral interactions between FtsZ protofilaments could be important for in vivo formation of the functional Z ring. However, the elements that play a key role in the Z-ring formation remain unknown.
In vitro, lateral interactions of FtsZ stabilize sheets and rings  and ZipA, mentioned earlier as a membrane anchor protein, stimulates the formation of FtsZ sheets . Moreover, FtsZ also polymerizes into sheets and tubules in the presence of cations such as calcium or DEAE-dextran [15–18], or by the FtsZ-interacting protein SepF [19, 20]. The sheets and tubules are stabilized by lateral interactions similar to those found in tubulin polymers [14, 21]. Moreover, arc-like filaments that could interact laterally have been observed by electron cryotomography at the constriction site , but the importance of these lateral interactions has remained controversial . Ca2+-induced FtsZ sheets are composed of protofilaments that are aligned in parallel and antiparallel way, while in Zn2+-induced tubulin sheets are arranged only in an antiparallel manner. Thus, from a low-resolution sheet structure of Methanococcus jannaschii FtsZ, it has been proposed that two protofilaments interact through the same lateral face, in a parallel form, to produce double-stranded filaments called thick filaments, which subsequently form sheets through antiparallel interactions . E. coli FtsZ also polymerases into thick filaments , indicating that these filaments can be the basic units to form sheets.
Phylogenetic and structural studies show that FtsZ is the most-conserved cell division protein and it shares structural and functional features with eukaryotic tubulins [25, 26]. The heterodimer αβ-tubulin polymerizes into microtubules and two types of interactions are involved between the heterodimer subunits: the longitudinal interactions between heterodimers to form the protofilaments, and the lateral interactions between 13 or 14 parallel protofilaments to organize into the microtubule. The lateral interactions result from the contacts between α- and β-tubulin subunits of neighboring protofilaments and are mediated by central structural elements, such as the interaction of the M loop of one subunit with the H3 helix of the neighboring subunit . A newly discovered bacterial tubulin, BtubA/B, also contains the H3 helix and M loop, however it is believed that the last one is a reminiscent structure from eukaryotic tubulin . Interestingly, the H3 helix is highly conserved among FtsZs while the M loop is absent. Therefore, we believe that H3 helix of FtsZ can participate in the lateral interactions in a similar manner to its related tubulins.
The aim of this work was to investigate the role of the H3 helix in lateral interactions of FtsZ from E. coli. We mutated the highly conserved glutamate 83 and arginine 85 residues to glutamine by site-directed mutagenesis and evaluated the effect of these mutations on the viability of the cells, the cell morphology and localization of FtsZ, as well as the GTPase activity and the ability to polymerize in vitro, in the presence and in the absence of calcium and ZipA.
Design of FtsZ(R85Q) and FtsZ(E83Q) mutants
We choose as candidates for mutation two conserved residues located in the lateral face of FtsZ, specifically in the bend of H3 helix. This was based on the similarities in the crystal structures between FtsZ and tubulin and the degree of conservation of amino acids among FtsZ sequences. The three dimensional structure of an E. coli FtsZ modela (Figure 1A) was compared with the structure of the αβ-tubulin heterodimer. The helix H3 of tubulin, located on the lateral face and known for the role in the lateral interactions, is also present in FtsZ. The helix H3 of FtsZ is located in the left lateral face (left, right, top and bottom face are the nomenclature defined by analogy between FtsZ protofilaments and tubulin microtubules) . Helix H3 is present in most of the FtsZ sequences from gram positive and negative bacteria, mitochondria and chloroplasts. Within the H3 helix, glutamate at position 83 is 100% conserved and the positive charge of arginine at 85 is highly conserved (80% using non-redundant sequences) among FtsZ sequences from 11 different organisms (Figure 1B). Interestingly, the two residues are located in the bend of the helix H3 and are exposed to the surface of the left face. Hence, we hypothesized that these residues, E83 and R85, participate in the interactions of FtsZ to form filaments, tubules or sheets. To this end, the bend in H3 could be necessary to maintain both the topography of the monomer for the interactions and the GTPase activity in the polymer. In microtubules, the lateral interactions are principally mediated between the helix H3 from a protofilament and the M loop of the adjacent protofilament . The M loop is even present in bacterial tubulin A/B, though smaller than its counterpart tubulin, and is also believed to participate in the lateral interactions to form a bacterial microtubules (bMTs) composed by five protofilaments . However, FtsZ lacks the M loop hence, the lateral interactions are expected to be weaker between FtsZ protofilaments , hence an effect on longitudinal interactions should affect both the length and the GTPase activity of the polymers.
Viability of E. coliVIP2(DE3) cells in the presence of FtsZ(E83Q) and FtsZ(R85Q)
To evaluate the functionality of the FtsZ mutants in vivo, the E83Q, and R85Q proteins were expressed independently and the cell viability was determined in a strain containing ftsZ thermonull mutant, VIP2(DE3)/pLAR9. The parental strain, VIP2/pLAR9 has been successfully employed to demonstrate that FtsZ is essential for cell division and survival in E. coli. For the purpose of our study we modified the parental strain to create a DE3 lysogen of VIP2, that contains a copy of T7 RNA polymerase gene under the control of PlacUV5, so the expression of a target protein is inducible by IPTG addition. Thus, if VIP2(DE3)/pLAR9 is transformed with a second plasmid that contains the FtsZ mutants (pMFV-derivatives: pMFV-wt, pMFV-rq and pMFV-eq, see Methods), the transformed cells will contain a ftsZ mutant allele from pMFV-derived plasmid under the control of a T7 promoter, a wild-type allele encoded on plasmid pLAR9 (a temperature sensitive replication plasmid) and a genomic null allele. In summary, at the permissive temperature (30°C), VIP2(DE3)/pLAR9/pMFV- will express both ftsZ alleles (from pLAR9 and pMFV-derivatives), whereas at the restrictive temperature (42°C) pLAR9 cannot replicate, so the ftsZ expression originates from the pMFV-derivatives. The production of FtsZ ceases completely after 2–3 h of shifting the temperature, once pLAR9 is segregated by lack of replication. In this regard, this system is different from those utilizing a FtsZ mutant nonfunctional at 42°C, because in this case after shifting to the non-permissive temperature FtsZ will be nonfunctional almost instantly.
To simplify the nomenclature we will refer henceforth to the strain VIP2(DE3), containing pLAR9, as VIP5.
VIP5/pMFV-wt cell grown at 42°C (positive control) and at 30°C show similar cfu/ml in the absence of IPTG, indicating that the basal non-induced expression level from pMFV-wt (a high copy number plasmid) was enough to complement the lack of FtsZ from pLAR9 at 42°C (Figure 2). Hence, in order to avoid an excessive expression of FtsZ, IPTG was not added in all our experiments. As expected, the survival of VIP5 at 42°C (negative control) decreases about four orders of magnitude. The small survival fraction observed in VIP5 is explained as due to the reversion frequency of the chromosomal mutation .
Figure 2 shows that the viability of cells expressing EcFtsZ mutants E83Q and R85Q at the restrictive temperature diminished about 1.5 and 3 orders of magnitude, respectively, compared to the non-restrictive temperature. These values indicate that the cells could not support normal cell division but at the same time the EcFtsZ mutants did not completely lose functionality in vivo. Hence, the survival assay was useful to characterize the functionality of EcFtsZ mutants in vivo and the quantification of the viability of the cells expressing EcFtsZ mutants was used as an indication of the efficiency in the cell division. Consequently, the efficiency of the different forms of EcFtsZ in terms of cell viability can be interpreted as: wild type > E83Q > R85Q.
Cell morphology and in situlocalization of FtsZ mutants using immunofluorescence microscopy
The FtsZ ring is formed in the middle of the cell and is attached to the cytoplasmic membrane through ZipA . In order to evaluate the effect of these mutations on cell morphology and FtsZ localization, VIP5 cells in exponential phase of growth carrying plasmids with FtsZ(WT), FtsZ(E83Q), and FtsZ(R85Q) mutants were incubated in the absence of IPTG at 30°C (control) and at 42°C. Figure 3 shows that, in the negative control at 42°C (pLAR9 is lost at this temperature) the cell division is blocked, and FtsZ localization is dispersed along the very long filamentous cells (a’), while at 30°C the cells are normal. Cells carrying the plasmid FtsZ(WT) at 42°C (b’) reverts the cell filamentation, and at 30°C (b) as expected the morphology and FtsZ localization is normal. The co-expression at 30°C of FtsZ(WT) from pLAR9 with the mutants FtsZ(E83Q) (c) and FtsZ(R85Q) (d) also display a normal phenotype. However, the expression of the FtsZ mutants at 42°C (in the absence of FtsZ(WT)) have an effect on cell division, producing filamentous cells (c’ and d’). Interestingly, FtsZ(E83Q) still localizes as discrete bands along the filamentous cells, probably at the potential cell division sites (c’). In contrast, the expression of FtsZ(R85Q) produced shorter filamentous cells with a disperse localization of the protein along the filaments, and no clear bands were observed (d’). The same experiments were performed with IPTG 0.2 mM and similar results were obtained (not shown). In order to keep the cells in exponential phase of growth for a longer time, the morphology experiments were repeated starting the culture for the temperature shift at an OD600 of 0.06. After 3 h of incubation no difference in the cell morphology of the mutants respect to the experiments presented in Figure 3 was observed.
Localization of the FtsZ mutants in the single cell population (not filaments) that appear at early stationary phase (Additional file 1: Figure S1) shows that FtsZ(E83Q) is located with greater intensity at the middle of the cell but presents some degree of dispersion, while FtsZ(R85Q) is rather distributed in a gradient shape from the mid-cell toward the cell poles. The ZipA distribution was similar to that observed for FtsZ mutants (Additional file 1: Figure S2), which suggests that the mutations in FtsZ do not affect its interaction with ZipA. Binding experiments between FtsZ (WT and mutants) and ZipA inserted into proteoliposomes confirmed this hypothesis (not shown).
In summary, the residues glutamate 83 and arginine 85 located in bend of helix H3 on the left face of FtsZ plays a role in the formation of the Z-ring (Figure 3) and consequently ZipA presents an altered localization in cells expressing the FtsZ mutants (Additional file 1: Figure S2).
Structural characterization of R85Q and E83Q
In order to evaluate the impact of the point mutations E83Q and R85Q on the protein structure, we performed circular dichroism on purified FtsZ wt, R85Q and E83Q. All three purified proteins showed the expected molecular mass and had a very similar secondary structure content (not shown); therefore, presumably the mutations did not cause significant changes in the tertiary structure of FtsZ.
GTPase activity of E83Q and R85Q
In order to understand the possible effects of the FtsZ mutations on the formation of the Z ring, we purified the mutant proteins and measured their abilities to hydrolyze GTP. The wild-type FtsZ and E83Q exhibited a constant initial velocity of GTP hydrolysis for at least 10 minutes at a protein concentration of 12.5 μM (Figure 4A). By contrast, R85Q showed a lag time (τ) of 86.3 minutes and a reduced GTPase activity. We next compared the GTP hydrolysis efficiency by calculating the apparent catalytic constants (i.e., the ratio between the initial velocity and the protein concentration). Wild-type FtsZ, E83Q and R85Q hydrolyzed GTP with apparent catalytic constants of 2.90 ± 0.16 min-1, 1.13 ± 0.06 min-1 and 0.13 ± 0.02 min-1 respectively. R85Q was further characterized by measuring the dependence of GTP hydrolysis on protein concentration (Figure 4B). The progress curves show a sigmoidal behaviour, i.e., the lag time was dependent on the protein concentration (inset Figure 4B). This lag time should disappear at the extrapolated concentration of 130 μM. The similarity of the progress curves at 36.8 and 49.0 μM of protein is probably due to GTP becoming limiting. These results suggest that the R85Q mutation does not directly alter the rate of GTP hydrolysis, but rather affects FtsZ polymerization, which is known to induce GTPase activity. Therefore, the effect is reversed when the concentration of R85Q is increased to stimulate polymerization.
The GTPase activity of FtsZ wt and E83Q is not affected by ZipA, which is in agreement with previous study . Interestingly, ZipA increased around twofold the GTPase activity of R85Q (Figure 4C and Additional file 1: Figure S3b), suggesting that ZipA stimulates the longitudinal interactions of R85Q protofilaments during polymerization, similar to the effect of MAPs on microtubules .
Polymerization of FtsZ mutants with and without ZipA
In vitro, FtsZ polymerizes into protofilaments in the presence of GTP , and these protofilaments associate to form bundles in the presence of ZipA . Single protofilaments are monomers that interact longitudinally whereas double protofilaments (thick filaments) consist of two parallel protofilaments interacting laterally . Antiparallel lateral interactions of these double filaments induce the formation of sheets or bundles . In order to evaluate the effect of the mutations on the polymerization capability, we followed the polymerization of FtsZ mutants by 90° light scattering at 350 nm and the polymers morphology by electron microscopy.
The polymerization reaction was started with the addition of the protein, and the reaction was followed for one hour for wild type and E83Q, while R85Q was followed for almost three hours due to the long lag period observed for the GTPase activity (Figure 4B). The mutant R85Q apparently did not form long filaments at the protein concentration used for FtsZ wild type and E83Q (Figure 5C), producing a small increment in turbidity that was stimulated around two-fold by ZipA Additional file 1: Figure S3a), whereas FtsZ wild type and E83Q showed a fast polymerization kinetics that could not be measured. E83Q formed polymers that remained in solution around three times longer than those formed by FtsZ wild type, with a rate of depolymerization 3.9 times slower. This increase in the time in which the filaments remain polymerized can be explained by the small apparent catalytic constant for the GTPase activity. The time traces of light scattering (Additional file 1: Figure S4) indicate that the polymerization and the subsequent depolymerization of FtsZ are altered by the mutations on the arginine 85 and glutamate 83. Additional file 1: Figure S5 shows that calcium increased the ligth scattering, and for FtsZ wild type and E83Q this was due to the formation of bundles, as shown in Additional file 1: Figure S6. On the other hand, R85Q presented a smaller increase in light scattering and no formation of bundles was observed.
Figure 5A shows the maximum light scattering value reached by FtsZ in 1 mM GTP in the presence and absence of ZipA. The mutants presented a decrease in the polymerization levels of ~20% and ~75% for E83Q and R85Q, respectively. In the presence of ZipA, the maximum light scattering value of FtsZ wild type increased slightly, (~20%, and bundles were observed by electron microscopy, Additional file 1: Figure S6), while for both mutants the increment was significantly larger: 90% and 150% for E83Q and R85Q, respectively (Figure 5A). In the absence of ZipA, FtsZ wild type polymerized into long and mostly straight filaments of 8 to 24 nm width (media around 14 nm) suggesting that the main population of these filaments consists of 4 assembled protofilaments of 4 nm width for each protofilament (Figure 5Ba and Ca'). E83Q polymerized into curved filaments with a width of 8 to 12 nm (Figure 5Bb and Cb'). The greatest effect was caused by the mutation R85Q, in which the polymers were mainly short and straight, and occasionally, spirals probably composed of single or double protofilaments were found on the grids (Figure 5Bc and Cc'). ZipA induced bundling of wt and E83Q, which is in agreement with previous studies ; however, we did not observe a clear bundling effect in the R85Q mutant, even given the increase in light scattering. The light scattering contribution of ZipA was subtracted, therefore we can rule out the possibility that the increase in the light scattering of R85Q is due to ZipA. Further characterization of R85Q will be done in a future study.
All the results together indicate that arginine 85 and glutamate 83 participate in the longitudinal and in the lateral interactions, shown by their reduced GTPase activity and the effect on polymerization, respectively. Interestingly, the addition of a stabilizer of the lateral interactions, such as ZipA to R85Q, did not induce bundles (Additional file 1: Figure S6) but stimulated the GTPase activity (Figure 4B and Additional file 1: Figure S3b), indicating that protofilaments are induced. On the other hand, in the case of E83Q, even though the protofilament lateral interactions were diminished (Figure 5B) and ZipA induced bundles that were thinner than those with FtsZ wild type, the GTPase activity was not greatly compromised. Therefore, we believe that the ability of FtsZ to form bundles, presumably through lateral interactions, is important to form the Z-ring in the cell, and consequently for cell division, a conclusion consistent with our immunofluorescence experiments (Figure 3) and cell viability results (Figure 2).
The aim of this work was to characterize the role of two conserved amino acid residues, E83 and R85, located in the bend of helix H3 at the lateral face of FtsZ (Figure 1A). Our results show that these amino acids are relevant for cell division, FtsZ polymerization, GTPase activity and stability of the polymers.
The properties of these FtsZ mutants were evaluated through their kinetics and critical concentrations for polymerization and GTPase activities in the absence and presence of ZipA and calcium. The FtsZ mutant proteins had the same secondary structure as the wild type form (within the experimental error) indicating an intact global fold. Interestingly, although the mutations are located far from the GTPase catalytic site (at the logitudinal interface within the filaments) both mutant proteins hydrolyzed GTP with less efficiency than wild type in the following order: WT > E83Q > R85Q. Compared to the wild type FtsZ, filaments with mutant proteins were thinner with altered curvatures, indicating that the mutations affect lateral interactions between FtsZ protofilaments. ZipA, which in wild type FtsZ is known to induce bundling without affecting GTPase activity, induced thinner bundles in E83Q, while in the case of R85Q was unable to cause bundling, suggesting that both glutamate 83 and arginine 85 are important for lateral interactions between protofilaments. R85Q filaments were short and abnormally bent, indicating that the mutation also affects the geometry of the longitudinal interactions. The formation of short filaments is consistent with the ability of R85Q to hydrolyze GTP, which requires longitudinal interaction between two FtsZ molecules [35–38]. Also the neighbor mutation D86K, which induces twined filaments, shows a reduction in GTPase activity further suggesting a role of this region in longitudinal interactions . The long lag period of the progress curve of R85Q GTPase activity was suppressed by increasing the protein concentration, confirming that this activity was directly related to polymerization. This result could be explained by a reduction of the nucleation free energy over the free energy for the elongation process.
The bend in H3 is likely caused by the negatively-charged amino acids DED located just before the bend which destabilize the helix by negative charge repulsion. At the other end of the H3 bend, R85 is followed by D86; therefore, the positive charge of R85 could destabilize the positive dipole of the helix in this region. Thus, altering the charges in this region could induce a conformational change, explaining the effects of the R85Q, E83Q and D86K  mutations on the functionality of FtsZ.
Based on our results we hypothesize that, directly or via a subtle conformational change, the charges of amino acids located in the bend of the H3 helix are involved in longitudinal and lateral interactions. We infer that the negative charge of glutamate 83 weakens these interactions while the positive charge of arginine 85 stabilizes them, probably regulating the dynamics of the Z-ring. Thus, cells carrying FtsZ(E83Q) or FtsZ(R85Q) were affected in cell division and showed reduced survival and cell filamentation in agreement with previously described lateral-interaction-FtsZ mutants that could not complement the temperature sensitive ftsZ84. We have quantified survival rates at the restrictive temperature (42°C) as a means to evaluate the effect of FtsZ mutations on cell division, similar to studies on the effects of proteins expression from unstable plasmids [39, 40].
The E83Q and R85Q mutations diminished the apparent catalytic constant of GTPase activity by 60% and 95%, respectively. Presumably, the positive charge of R85 is involved in longitudinal and lateral interactions between protofilaments explaining the inhibitory effect of the R85Q mutation on GTPase activity and polymerization when compared to the E83Q or D86K mutants (Table 1) . Both, longitudinal and lateral interactions are important in models for the conversion of GTP hydrolysis into mechanical energy during Z-ring constriction [8, 25]. The observed contraction of tubular liposomes in the presence of GTP and a chimeric, membrane-attached FtsZ indicates that polymerization of FtsZ is sufficient to generate a mechanical force to bend the membrane. Our results indicate that H3 bend mutations affecting longitudinal and lateral interactions in vitro reduce cell viability in vivo while they cause cell filamentation. Cell filamentation and the immunofluorescence analysis (Figure 3) together with the polymerization results support a model of a Z-ring composed of several polymers that are recruited to the septation site by lateral interactions, as has been found in vivo.
This study describes the role of the positively charged arginine 85 and the negatively charged glutamate 83 in longitudinal and lateral interactions to form FtsZ protofilaments and two-dimensional polymers of protofilaments, respectively. We also show that GTPase activity could be fine-tuned by the net charge of the H3 helix bend. Finally, our results suggest that the conserved amino acids E83Q and R85Q located in the bend of H3 are involved in the formation of a functional Z-ring.
Bacterial strains and plasmids
Strain C41(DE3), cured from C41/pHis17-MJ0370 , was used to express E. coli FtsZ WT and mutants. VIP2(DE3)/pLAR9 was used to evaluate the functionality of FtsZ in vivo. VIP2(DE3)/pLAR9 is VIP2/pLAR9  lysogenized with the λDE3 Lysogenization Kit (Novagen).
pMFV57 was constructed from pMFV56  by cloning the EcoRV β-lactamase fragment into the kanamycinr (kan) gene. The β-lactamase gene was generated by PCR using the following primers: 5′-CATTCAAATATGGATCCGCTCATG-3′ and 5′-ACCAATGCTTAATCAGTGAGG-3′. FtsZ E83Q and R85Q mutants were constructed using the QuikChange XL Site-Directed Mutagenesis Kit (Stratagene) and the pairs of primers used to generate the mutations were the following: 5′-GCAATGCGGCTGATCAGGATCGCGATGCATTGC-3′ and 5′-GCAATGCATCGCGATCCTGATCAGCCGCATTGC-3′; 5′-GCGGCTGATGAGGATCAGGATGCATTGCGTGCG-3′ and 5′-CGCACGCAATGCATCCTGATCCTCATCAGCCGC-3′, respectively. The presence of the ftsZ gene mutations was confirmed by DNA sequencing and the new plasmids were named pMFV-E83Q and pMFV-R85Q. These constructions are derivatives of the pET28 expression system; FtsZ expression is thus inducible by the addition of IPTG.
E. coli FtsZ(WT), FtsZ(E83Q) and FtsZ(R85Q) mutants were purified as described previously  with some modifications. E. coli C41(DE3) carrying the appropriate pMFV-plasmid derivative was grown in LB-ampicilin (amp) with shaking at 37°C. When the OD550 reached 0.5, IPTG was added to a final concentration of 0.5 mM and the culture was grown for a further 3 h. Harvested cells were washed and then suspended in buffer A (50 mM Tris–HCl, pH 7.9, 50 mM KCl, 1 mM ethylenediaminetetraacetic acid (EDTA), 10% glycerol). After lysis by sonication (Sonifier Cell Disruptor Model W185, 1 cm diameter), the suspension was centrifuged at 28,000 rpm, at 4°C for 90 min (Beckman L5-75B Ultracentrifuge, rotor type 30) to remove the membrane fraction and the supernatant was collected. The proteins were precipitated by adding 35% ammonium sulfate, centrifuged in the same rotor at 15,000 rpm for 20 min and the pellet was dissolved in buffer A. The dialyzed ammonium sulfate fraction was loaded onto a Q-Sepharose column (25 cm × 4.6 mm) and eluted with a 50 mM – 1 M gradient of KCl at a flow rate of 1 ml/min. The FtsZ fractions were loaded onto a Sephacryl S-400 column and eluted with buffer A at a flow rate of 1 ml/min. The peak fractions were pooled, concentrated to 10 – 15 mg protein/ml using Centriprep, fast frozen and stored at −80°C. The E. coli FtsZ obtained was pure, as determined by SDS-PAGE gels and western blotting.
In vivocell survival assay
To evaluate the functionality of the FtsZ(E83Q) and FtsZ(R85Q), the viability of cells expressing these mutants was determined using the strain E. coli VIP2(DE3)/pLAR9 (also called VIP5). In this strain the ftsZ chromosomal copy is interrupted by an insertion (resistant to kanamycin (kan)), and complemented with a copy encoded on the thermosensitive plasmid pLAR9, resistant to chloramphenicol (cam) . The assay was performed as described by Díaz-Espinoza et al. . Briefly, overnight cultures of VIP5 transformed with or without pMFV-WT, pMFV-E83Q or pMFV-R85Q, were diluted 1:100 in LB media containing 100 μg/ml amp and grown at 30°C until the OD550 reached 0.5 – 0.6. Serial dilutions of this culture plated on LB-amp were incubated, at both the permissive (30°C) and non-permissive temperatures (42°C). The next day, the survival was determined for wtFtsZ and each of the mutants.
GTPase activity was monitored by determining the area under the peaks corresponding to GDP and GTP from the HPLC chromatograms obtained after loading the de-proteinized reaction samples onto a SUPERCOSIL LC-18-DB column (SUPELCO) as previously described . FtsZ was incubated for 2 min in polymerization buffer (50 mM Mes pH 6.5, 50 mM KCl, 10 mM MgCl2) at 30°C and the reaction was started by addition of GTP (1 mM final concentration). At different times, 100 μl samples were withdrawn and transferred to 8 μl 70% perchloric acid and neutralized by the addition of 100 μl 1.2 M K2CO3. After degasification in a LABCONCO Speed-Vac at room temperature for 5 min, the reaction was centrifuged at 14,000 rpm for 15 min and a 20 μl aliquot of the supernatant was loaded onto the SUPERCOSIL LC-18-DB column and eluted at 1 ml/min using a buffer containing 0.2 M K2HPO4, 0.1 M acetic acid and 4 mM tetrabutylammonium phosphate monobasic (TBAP) in an HPLC. GDP and GTP were detected at 260 nm by a UV detector system. Alternatively, the inorganic phosphate product of GTP hydrolysis was determined using the colorimetric method of malachite green . In brief, the polymerization sample (70 μL) was mixed with perchloric acid (10% final concentration). After 5 minutes, 50 μL of this solution were mixed in a vortex for 1 min with 800 μl malachite green reagent 0.045%. 100 μl of citrate (34%) were added and the solution was kept on ice for 20 min before the measurement of the absorbance at 630 nm.
E. coliFtsZ polymerization by light scattering
Assembly of FtsZ was monitored using the Luminescence Spectrometer LS 50 (Perkin Elmer) as described previously . Excitation and emission wavelengths were set to 350 nm, with a slit width of 5 nm. 12.5 μM FtsZ was incubated in polymerization buffer at 30°C in a fluorescence cuvette with a 1 cm path length and after 2 min of data collection, GTP was added to a final concentration of 1 mM.
Immunofluorescence of E. coliFtsZ and ZipA
To label FtsZ and ZipA, cells were treated following the method described by Rueda et al. . This method has three steps: fixation, permeabilization and development with specific antibodies. A) Fixation. In order to compare the localization of the proteins at 30°C and 42°C, duplicated cellular cultures of VIP2/pLAR9, complemented with FtsZ(WT) and the mutants FtsZ(E83Q) or FtsZ(R85Q) were grown. The cultures grown at 30°C contained the antibiotics kan, amp and cam, while cultures grown at 42°C only contained kan and amp. The negative control was the strain VIP2/pLAR9 without pMFV57, and no amp was added to the culture. After the cell culture reached an OD600 of 0.5, one of the duplicated tubes was incubated at 42°C and the growth continued for another 4 h. The cells were fixed with 0.75% formaldehyde and incubated for 10 min at room temperature followed by 30 min on ice. The fixed cells were washed with PBS and suspended in buffer GTE (20 mM Tris–HCl pH 7.5, 50 mM glucose and 10 mM EDTA). B) Permeabilization. 8 μg/ml final concentration of lysozyme were added to 30 μl of fixed cells, placed on a glass slide coated with poly-L-lysine and incubated at room temperature for 2–3 min and washed with PBS. C) Development was carried out with specific antibodies against FtsZ and ZipA. The unspecific binding sites were blocked by adding 3% BSA in PBS for 15 min at room temperature and this solution was replaced by a solution containing an appropriate dilution of the primary polyclonal antibody (anti-FtsZ or anti-ZipA). After an overnight incubation at 4°C, the cells were washed with PBS and incubated for 2 h with the secondary antibody (anti-rabbit labeled with Alexa 488, Invitrogen) at room temperature in darkness. After several washes with PBS, a drop of antifading (Vectors Lab) was added and the samples covered with a cover slide. Cells were observed in a confocal microscope (Leica TCS SP2).
Staining and visualization of the FtsZ polymers were carried out in a JEOL JEM-100SX electron microscope. The FtsZ polymers in absence or presence of ZipA were incubated at 30°C in polymerization buffer with 1 mM GTP. After the maximal polymerization was reached (as detected by light scattering), 10 μl of the polymerization reaction was placed on a carbon-coated grid for 1 min and stained with 1% of aqueous uranyl acetate. Electron micrographs were taken at different magnifications. Each picture was digitalized directly from the film and the width of the polymers was measured using the computer program ImageJ 1.34 s (http://rsb.info.nhi.gov/ij/download.htlm).
a The 3D structure of the E. coli FtsZ model was built with the program MODELLER v6.2 using as template the crystal structures of M. jannaschii FtsZ (1FSZ.pdb) and P. aureginosa FtsZ (1OFU.pdb). The program gave a rms value of 0.31 Å for the structural fit. The good quality of the model was assessed with the computer programs PROSA II and Verify 3D (web site http://nhiserver.mbi.ucla.edu/Verify_3D/).
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We thank Drs. David Jameson and Michael Handford for the critical review of the manuscript. Andrea Poch and Felipe Hurtado for cell morphology experiments (not shown), Christian Escobar and Gissela Araya for polymerization experiments (not shown). We are grateful to Dr. Debabrata RayChaudhuri for the ZipA plasmid donation and Dr. Carlos Bustamante for the use of his microscope facility for cell morphology studies. This work was supported by EC FP7 Grant DIVINOCELL # 223431 and FONDECYT Grant # 1095121. The stay of J.Y. Shin in the laboratory of Dr. W. Vollmer was financed by DAAD.
Authors and Affiliations
Department of Physics, University of California, Berkeley
Jae Yen Shin
Institute for Cell and Molecular Biosciences, Centre for Bacterial Cell Biology, Newcastle University, Richardson Road, NE2 4AX, Newcastle upon Tyne, UK
Departamento de Biología, Facultad de Ciencias, Casilla 653, Santiago, Chile
JYS contributed to the conception and design of the study, and conducted the experiments. OM and RL conceived the study, designed the experiments, analyzed and interpreted the data. WV designed, conducted and supervised the electron and confocal microscopy experiments. JYS, WV, RL and OM wrote the manuscript. All authors have no conflicts of interest to report. All authors read and approved the final manuscript.
Additional file 1: Figure S1: Cellular localization and distribution of FtsZ WT and the mutants E83Q and R85Q. Figure S2. Localization and distribution of ZipA in cells expressing FtsZ WT and mutants E83Q and R85Q. Figure S3. Effect of ZipA on the polymerization of FtsZ R85Q using light scattering and GTPase activity. Figure S4. Polymerization kinetics of FtsZ WT and the mutants E83Q and R85Q detected by light scattering. Figure S5. Effect of Ca2+ on the polymerization of FtsZ WT and the mutants E83Q and R85Q using light scattering. Figure S6. Effect of Ca2+ and ZipA on the polymers morphology of FtsZ WT and the mutants E83Q and R85Q. (PDF 9 MB)
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Shin, J.Y., Vollmer, W., Lagos, R. et al. Glutamate 83 and arginine 85 of helix H3 bend are key residues for FtsZ polymerization, GTPase activity and cellular viability of Escherichia coli: lateral mutations affect FtsZ polymerization and E. coliviability.
BMC Microbiol13, 26 (2013). https://doi.org/10.1186/1471-2180-13-26