Figure 2

Putative predicted operons: Predicted operon examples for four of the extra cellular proteins found in the LAB spp. Each picture displays the surrounding genes or operon as well as gene location. The first example is a 60 kDa chaperonin (RFYD01561, [GenBank: KC776105]) predicted operon from Lactobacillus Bin4N, involving the cistrons that form the predicted operon. The red arrow is the extra-cellularly identified chaperonin GroEL, while the grey arrow is the other predicted cistron that forms the putative operon (chaperonin GroES). The red arrow is the extra-cellularly produced enzyme pyruvate kinase while the grey arrows are the other predicted cistrons that form the putative operon. The second is an example of enzyme pyruvate kinase (RYBW00366, [GenBank: KC789985]) predicted from Lactobacillus Hon2N operon, involving cistrons that form the predicted operon. The third set of arrows is an example of an S-layer protein (RNKM00463, [GenBank: KC776070]) predicted from a Lactobacillus Hma11N operon, involving the genes that form the predicted operon and the surrounding genes of interest. Interestingly this putative SLP is not part of an operon but surrounded by two operons. The predicted operon can be seen in grey. The red arrow displays an example of the SLP that is extra-cellularly produced. The last set of arrows displays the putative surrounding genes for the Helveticin J homolog (RLTA01902, [GenBank: KC776075]) that was identified in Lactobacillus Bma5N. This putative bacteriocin (red arrow) does not form part of an operon but is surrounded by an S-layer protein and unknown protein (grey arrows).