Table 1 S. cerevisiae strains used in this study
Strain designation | Genotype | Transformed with | Reference |
|---|---|---|---|
BWG1-7a | Mat a ura3-52 leu2-3,112 his4-519 ade1-100 | - | [38] |
YES | BWG1-7a | pYES2 | This study |
NIK | BWG1-7a | pYES2-CaNIK1-TAG | [25] |
H510 | BWG1-7a | pYES2-CaNIK1(H510Q) | This study |
D924 | BWG1-7a | pYES2-CaNIK1(D924N) | This study |
N627 | BWG1-7a | pYES2-CaNIK1(N627D) | This study |
ΔHa | BWG1-7a | pYES2-CaNIK1ΔHAMP | This study |
ΔHaH510 | BWG1-7a | pYES2-CaNIK1ΔHAMP(H510Q) | This study |
ΔH3H4 | BWG1-7a | pYES2-CaNIK1Δ224-315Δ327-418aa | [27] |
BY4741 | Mat a his3Δ 1; leu2Δ 0; met15Δ 0; ura3Δ 0 | - | [39] |
ΔHb | BY4741 | pYES2-CaNIK1ΔHAMP | This study |
ΔHbH510 | BY4741 | pYES2-CaNIK1ΔHAMP(H510Q) | This study |
Δssk1 | BY4741, YLR006c::kanMX4 | - | [49] |
Δpbs2 | BY4741, YJL128c::kanMX4 | - | [49] |
Δhog | BY4741, YLR113w::kanMX4 | - | [49] |
ΔHbΔssk1 | Δssk1 | pYES2-CaNIK1ΔHAMP | This study |
ΔHbΔpbs2 | Δpbs2 | pYES2-CaNIK1ΔHAMP | This study |
ΔHbΔhog | Δhog | pYES2-CaNIK1ΔHAMP | This study |