Figure 5
![Figure 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1471-2180-13-209/MediaObjects/12866_2013_Article_2079_Fig5_HTML.jpg)
The MAPK Hog1p was constitutively phosphorylated after expression of CaNIK1ΔHAMP in the strain ΔHa. Phosphorylation of Hog1p (upper panel, Hog1-P) in the strains YES, NIK, ΔHa and ΔHaH510 was detected after cultivation of the strains in SG-ura for 195 and 210 min. Fludioxonil was added after 180 min at a final concentration of 10 μg/ml. The presence of Hog1p (lower panel, Hog1) was confirmed in all strains. Hog1p appears at approximately 50 kDa.