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Figure 3 | BMC Microbiology

Figure 3

From: Enzymatically active Rho and Rac small-GTPases are involved in the establishment of the vacuolar membrane after Toxoplasma gondii invasion of host cells

Figure 3

The recruitment of RhoA and Rac1 GTPases into parasitophorous vacuole membrane (PVM) is dependent on the GTPase activity (1000×). (A) The CFP-tagged dominant negative mutants RhoA N19 and Rac1 N17 were overexpressed in COS-7 cells and 48 hr post-transfection, the cells were infected with T. gondii RH tachyzoites. All of these mutant proteins did not accumulate on the PVM of T. gondii (arrowhead). (B) The CFP-tagged wild type RhoA and Rac1 were overexpressed in COS-7 cells and 48 hr post-transfection, the cells were infected with T. gondii RH tachyzoites. All of these wild-type proteins accumulated on the PVM of T. gondii (arrowhead). Bars: 10 μm.

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BMC Microbiology

ISSN: 1471-2180

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