TbLpn sequence analysis. A) Shown is the predicted amino acid sequence of TbLpn. The region shown to interact with TbPRMT1 by yeast-two hybrid analysis is shown in italics. The N-LIP and C-LIP domains are boxed. The conserved DxDxT domain is shown in bold. B) A schematic representation of TbLpn amino acid sequence aligned with members of the lipin family is shown in the left panel. The degree of amino acid sequence identity between TbLpn and members of the lipin family is shown on the right panel. TbLpn [T. brucei, (Tb), accession number AAX78871], Lipin-1 [Human, (Hs), AAH30537], Lipin-2 [Human, (Hs), AAI52449], Lipin-3 [Human (Hs), CAI42978], Lipin-1 [Mouse, (Mm), NP_766538], Lipin-2 [Mouse (Mm), AAH39698], Lipin-3 [Mouse (Mm), EDL06298], Lipin-M [Drosophila melanogaster, (Dm), NP_001188884], Lipin-1 [Danio rerio, (Dr), AAX19945], Smp2 [Saccharomyces cerevisiae, (Sc), BAA00880].