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Table 1 Mean values and statistics of soil DNA extractions and real time PCRs

From: Development and validation of a real-time PCR assay for detection and quantification of Tuber magnatum in soil

Truffière locality (region) Soil DNA extraction1 PP/TNP Real time data1
  quantity (μg g-1 soil)2 OD260/230 nm OD260/280 nm   plot with TM-DNA/TNP TM-DNA concentration3
       Whole2 PP NPP4
Feudozzo (A) 3.4 a 1.75 1.79 6/12 12/12 8.46 a 9.85 7.08
Collemeluccio (M) 2.3 a 1.64 1.64 1/9 5/9 0.72 a 3.12 0.03*
Argenta (ER) 6.9 b 1.81 1.83 4/9 8/9 11.76 a 19.28 5.73*
Barbialla (T) 7.0 b 1.82 1.83 6/9 9/9 28.18 b 35.41 13.71
  1. 1Mean values referred to three years of experimentation.
  2. 2Different letters in the same column indicate significant differences between the mean values obtained from different truffières (ANOVA and Bonferroni’s test, p < 0.05).
  3. 3pg of T. magnatum DNA in 200 ng of total DNA.
  4. 4 The asterisk indicates significant differences between the mean TM-DNA concentration of PP and NPP in the same truffière (ANOVA, p < 0.05).
  5. A, Abruzzo; M, Molise; ER, Emilia Romagna; T, Tuscany; OD, optical density; PP, productive plots; NPP, non productive plots; TNP, total number of plots; TM-DNA, T. magnatum DNA.