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Table 1 Mean values and statistics of soil DNA extractions and real time PCRs

From: Development and validation of a real-time PCR assay for detection and quantification of Tuber magnatum in soil

Truffière locality (region)

Soil DNA extraction1

PP/TNP

Real time data1

 

quantity (μg g-1 soil)2

OD260/230 nm

OD260/280 nm

 

plot with TM-DNA/TNP

TM-DNA concentration3

      

Whole2

PP

NPP4

Feudozzo (A)

3.4 a

1.75

1.79

6/12

12/12

8.46 a

9.85

7.08

Collemeluccio (M)

2.3 a

1.64

1.64

1/9

5/9

0.72 a

3.12

0.03*

Argenta (ER)

6.9 b

1.81

1.83

4/9

8/9

11.76 a

19.28

5.73*

Barbialla (T)

7.0 b

1.82

1.83

6/9

9/9

28.18 b

35.41

13.71

  1. 1Mean values referred to three years of experimentation.
  2. 2Different letters in the same column indicate significant differences between the mean values obtained from different truffières (ANOVA and Bonferroni’s test, p < 0.05).
  3. 3pg of T. magnatum DNA in 200 ng of total DNA.
  4. 4 The asterisk indicates significant differences between the mean TM-DNA concentration of PP and NPP in the same truffière (ANOVA, p < 0.05).
  5. A, Abruzzo; M, Molise; ER, Emilia Romagna; T, Tuscany; OD, optical density; PP, productive plots; NPP, non productive plots; TNP, total number of plots; TM-DNA, T. magnatum DNA.
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BMC Microbiology

ISSN: 1471-2180

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