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Figure 1 | BMC Microbiology

Figure 1

From: Development and validation of a real-time PCR assay for detection and quantification of Tuber magnatum in soil

Figure 1

PCR sensitivity of the primer pairs selected from ITS1 and ITS2 regions. Reactions carried out using serial dilutions of T. magnatum DNA (TM-DNA) in pooled non-target fungal DNAs (F-DNA): lane M, Mass ruler marker (Fermenats); lanes 1, 3, 5 and 7, ITS1for-ITS1rev primer pair; lanes 2, 4, 6 and 8, ITS2for-ITS2rev primer pair. Lanes 1–2, 10 ng TM-DNA/90 ng F-DNA; lanes 3–4, 1 ng TM-DNA/99 ng F-DNA; lanes 5–6, 0.1 ng TM-DNA/99.9 ng F-DNA; lanes 7–8, 0.01 ng TM-DNA/99.99 ng F-DNA.

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BMC Microbiology

ISSN: 1471-2180

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