The RNA processing enzyme polynucleotide phosphorylase negatively controls biofilm formation by repressing poly-N-acetylglucosamine (PNAG) production in Escherichia coli C
BMC Microbiology volume 12, Article number: 270 (2012)
Transition from planktonic cells to biofilm is mediated by production of adhesion factors, such as extracellular polysaccharides (EPS), and modulated by complex regulatory networks that, in addition to controlling production of adhesion factors, redirect bacterial cell metabolism to the biofilm mode.
Deletion of the pnp gene, encoding polynucleotide phosphorylase, an RNA processing enzyme and a component of the RNA degradosome, results in increased biofilm formation in Escherichia coli. This effect is particularly pronounced in the E. coli strain C-1a, in which deletion of the pnp gene leads to strong cell aggregation in liquid medium. Cell aggregation is dependent on the EPS poly-N-acetylglucosamine (PNAG), thus suggesting negative regulation of the PNAG biosynthetic operon pgaABCD by PNPase. Indeed, pgaABCD transcript levels are higher in the pnp mutant. Negative control of pgaABCD expression by PNPase takes place at mRNA stability level and involves the 5’-untranslated region of the pgaABCD transcript, which serves as a cis-element regulating pgaABCD transcript stability and translatability.
Our results demonstrate that PNPase is necessary to maintain bacterial cells in the planktonic mode through down-regulation of pgaABCD expression and PNAG production.
Most bacteria can switch between two different lifestyles: single cells (planktonic mode) and biofilms, i.e., sessile microbial communities. Planktonic and biofilm cells differ significantly in their physiology and morphology and in their global gene expression pattern [1–3]. Extensive production of extracellular polysaccharides (EPS) represents a defining feature of bacterial biofilms; EPS are the major constituent of the so-called “biofilm matrix”, which also includes cell surface-associated proteins and nucleic acids [4, 5]. In addition to constituting the material embedding biofilm cells and to being a main determinant for surface attachment, the EPS are responsible for cell resistance to environmental stresses such as desiccation  and to predation by bacteriophages . In several bacterial species, EPS are also required for swarming motility [8, 9].
Expression of genes involved in EPS biosynthesis is controlled by complex regulatory networks responding to a variety of environmental and physiological cues, including stress signals, nutrient availability, temperature, etc. [10–13]. Regulation of EPS production can take place at any level, i.e., transcription initiation, mRNA stability, and protein activity. For instance, the vps genes, involved in EPS biosynthesis in Vibrio cholerae, are regulated at the transcription level by the CytR protein, in response to intracellular pyrimidine concentrations . The RsmA protein negatively regulates EPS production in Pseudomonas aeruginosa by repressing translation of the psl transcript . Finally, cellulose production in Gluconacetobacter xylinum and in various enterobacteria requires enzymatic activation of the cellulose biosynthetic machinery by the signal molecule cyclic-di-GMP (c-di-GMP) [16, 17], a signal molecule which plays a pivotal role as a molecular switch to biofilm formation in Gram negative bacteria . The great variety of regulatory mechanisms presiding to EPS biosynthesis, and the role of c-di-GMP as signal molecule mainly devoted to its control, underline the critical importance of timely EPS production for bacterial cells.
Polynucleotide phosphorylase (PNPase) plays an important role in RNA processing and turnover, being implicated in RNA degradation and in polymerization of heteropolymeric tails at the 3’-end of mRNA [19, 20]. PNPase is an homotrimeric enzyme that, together with the endonuclease RNase E, the DEAD-box RNA helicase RhlB, and enolase, constitute the RNA degradosome, a multiprotein machine devoted to RNA degradation [21, 22]. Despite the crucial role played by PNPase in RNA processing, the pnp gene is not essential; however, pnp inactivation has pleiotropic effects, which include reduced proficiency in homologous recombination and repair [23, 24], inability to grow at low temperatures  and inhibition of lysogenization by bacteriophage P4 . Moreover, lack of PNPase affects stability of several small RNAs, thus impacting their ability to regulate their targets .
In this work, we show that deletion of the pnp gene results in strong cell aggregation and biofilm formation, due to overproduction of the EPS poly-N-acetylglucosamine. Increased biofilm formation was observed both in E. coli MG1655 and C-1a strains, being more pronounced in the latter. We demonstrate that PNPase negatively controls expression of the PNAG biosynthetic operon pgaABCD at post-transcriptional level, thus acting as a negative determinant for biofilm formation. Our observation that PNPase acts as an inhibitor of biofilm formation is consistent with previous findings highlighting the importance of regulation of EPS production and biofilm formation at mRNA stability level .
Bacteria and growth media
Bacterial strains and plasmids are listed in Table 1. E. coli C-1a is a standard laboratory strain , whose known differences with E. coli MG1655 reside in its restriction/modification systems  and in the presence of a functional rph gene, encoding ribonuclease PH, which, in contrast, is inactivated by a frameshift mutation in E. coli MG1655 . For strain construction by λ Red-mediated recombination , if not otherwise indicated, the parental strains were transformed with DNA fragments obtained by PCR using either pKD3 (for amplification of DNA fragments carrying chloramphenicol-resistance cassettes) or pKD13 (for DNA fragments carrying kanamycin-resistance cassettes) as template. The sequences of oligonucleotides utilized in this work are reported in Additional file 1: Table S1. Bacterial cultures were grown in the following media: LD (10 g/l tryptone, 5 g/l yeast extract, 5 g/l NaCl); M9 (82 mM Na2HPO4, 24 mM KH2PO4, 85 mM NaCl, 19 mM NH4Cl, 1 mM MgSO4, 0.1 mM CaCl2, 0.1 μg/ml thiamine); M9/sup (M9 supplemented with 0.25 g/l tryptone, 0.125 g/l yeast extract, 0.125 g/l NaCl). Unless otherwise stated, 0.4% glucose was added to give either M9Glu or M9Glu/sup media. When needed, media were supplemented with 100 μg/ml ampicillin.
Cell aggregation and adhesion assays
Cell aggregation was assessed as follows: overnight cultures grown in LD at 37°C on a rotatory device were diluted 50-fold in 50 ml of M9Glu/sup in a 250 ml flask. The cultures were then incubated at 37°C with shaking at 100 rpm. Cell adhesion to the flask walls was assessed in overnight cultures grown in M9Glu/sup medium at 37°C. Liquid cultures were removed and cell aggregates attached to the flask glass walls were stained with crystal violet for 5 minutes to allow for better visualization. Quantitative determination of surface attachment to polystyrene microtiter wells was carried out using crystal violet staining as previously described . Binding to Congo red (CR) was assessed in CR agar medium (1% casamino acid, 0.15% yeast extract, 0.005% MgSO4, 2% agar; after autoclaving, 0.004% Congo red and 0.002% Coomassie blue). Overnight cultures in microtiter wells were replica plated on CR agar plates, grown for 24 h at 30°C, and further incubated 24 h at 4°C for better detection of staining.
Gene expression determination
RNA extraction, Northern blot analysis and synthesis of radiolabelled riboprobes by in vitro transcription with T7 RNA polymerase were previously described [34, 35]. The DNA template for PGA riboprobe synthesis was amplified by PCR on C-1a genomic DNA with oligonucleotides FG2491/39 and FG2492/22. Autoradiographic images of Northern blots were obtained by phosphorimaging using ImageQuant software (Molecular Dynamics). Quantitative (real time) reverse transcriptase PCR (quantitative RT-PCR) was performed as described . Oligonucleotides PL101/21 and PL102/19 were used for 16S rRNA reverse transcription and PCR amplification. mRNA half-lives were estimated as described  by regression analysis of mRNA remaining (estimated by real time PCR) versus time after rifampicin addition. Luciferase assays were performed as in . Oligonucleotides utilized for Northern blot, real time PCR, and construction of reporter plasmids are listed in Additional file 1: Table S1.
PNAG production was determined as described . Bacteria were grown overnight in 3 ml of M9 Glu/sup medium at 37°C. Cells were collected by centrifugation and diluted in Tris-buffered saline [20 mM Tris–HCl, 150 mM NaCl (pH 7.4)] to an OD600 = 1.5. 1ml of suspension was centrifuged at 10,500 x g, resuspended in 300 μl of 0.5 M EDTA (pH 8.0), and incubated for 5 min at 100°C. Cells were removed by centrifugation at 10,500 x g for 6 min and 100 μl of the supernatant was incubated with 200 μg of proteinase K for 60 min at 60°C. Proteinase K was heat-inactivated at 80°C for 30 min. The solution was diluted 1:3 in Tris-buffered saline and 10 μl was spotted onto a nitrocellulose filter using a Dot-blot apparatus (Bio-Rad). The filter was saturated for about 2 hours in 0.1 M Tris–HCl (pH 7.5), 0.3 M NaCl, 0.1% Triton (Sigma Aldrich) and 5% milk and then incubated overnight at 4°C with a 1:1,000 dilution of purified PNAG antibodies (a kind gift from G.B. Pier ). PNAG antibodies were detected using a secondary anti-goat antibody (dilution 1:5,000) conjugated with horseradish peroxidase. Immunoreactive spots were revealed using ECL Western blotting reagent (Amersham Pharmacia Biotech).
When applicable, statistically significant differences among samples were determined using a t-test of analysis of variance (ANOVA) via a software run in MATLAB environment (Version 7.0, The MathWorks Inc.). Tukey’s honestly significant different test (HSD) was used for pairwise comparison to determine significance of the data. Statistically significant results were depicted by p-values <0.05.
Lack of PNPase induces cell aggregation in E. coli C
The E. coli C pnp deletion mutant C-5691 (a derivative of E. coli C-1a [40, 41]) showed an apparent growth arrest when grown at 37°C in M9 minimal medium with glucose as sole carbon source (M9Glu, Figure 1A, left panel). The growth defect was overcome by supplementing M9Glu with 0.25 g/l tryptone, 0.125 g/l yeast extract, 0.125 g/l NaCl (M9Glu/sup medium); however, in such conditions, C-5691 optical density drastically decreased at the onset of stationary phase. Such drop was due to cell flocculation, leading to formation of macroscopic cell clumps sedimenting onto the flask glass wall (Figure 1A, right panel). Cell flocculation also occurred when either arabinose or glycerol were added to M9/sup media instead of glucose (data not shown).
The aggregative phenotype of the C-5691 (Δpnp) strain was complemented by basal expression from a multicopy plasmid of the pnp gene under araBp promoter, indicating that low PNPase expression is sufficient to restore planktonic growth. Conversely, arabinose addition did not completely restore a wild type phenotype (Figure 1B, left panel), suggesting that PNPase overexpression may also cause aggregation. Ectopic expression of RNase II suppressed the aggregative phenotype of the pnp mutant (Figure 1B, right panel), thus suggesting that such a phenotype is controlled by the RNA degrading activity of PNPase. In contrast, however, RNase R overexpression did not compensate for lack of PNPase, indicating that different ribonucleases are not fully interchangeable in this process.
Inactivation of the pnp gene induces poly-N-acetylglucosamine (PNAG) production
In addition to macroscopic cell aggregation (Figures 1 and 2A), deletion of pnp stimulated adhesion to polystyrene microtiter plates in a standard biofilm formation assay  (Figure 2B) and resulted in red phenotype on solid medium supplemented with Congo red, a dye binding to polymeric extracellular structures such as amyloid fibers and polysaccharides (Figure 2C). Cell aggregation was also observed by phase contrast microscopy (Figure 2D). Altogether, these observations strongly suggest that inactivation of pnp triggers the expression of one or more extracellular factors implicated in cell aggregation and adhesion to solid surfaces. In order to identify such factor(s), we searched for deletion mutants in genes encoding known adhesion factors and biofilm determinants that could suppress the aggregative phenotype of the C-5691 (Δpnp) mutant strain. The following adhesion factors were targeted by appropriate mutations (Table 1): curli fibers (ΔcsgA), which strongly promote attachment to abiotic surfaces and constitute the main determinant for Congo red binding [42, 43]; cellulose (ΔbcsA) and PNAG (ΔpgaA and ΔpgaC), two extracellular polysaccharides able to promote surface adhesion and to affect Congo red binding to the bacterial cell [44, 45]; and the capsular polysaccharide colanic acid (ΔwcaD), which promotes biofilm maturation acting synergistically with other adhesion factors such as curli fibers or conjugative pili [46, 47].
The aggregative phenotype of the C-5691 (Δpnp) mutant, as determined by cell aggregation, surface adhesion, and Congo red binding experiments, was totally abolished by deletion of pgaC (Figure 2), which encodes the polysaccharide polymerase needed for biosynthesis of PNAG from UDP-N-acetylglucosamine . Deletion of pgaA, also part of the PNAG biosynthetic operon pgaABCD, produced identical effects as pgaC (data not shown). In contrast, no significant effects on either Congo red binding or cell aggregation and adhesion were detected in any Δpnp derivative unable to produce curli or colanic acid (Figure 2). Finally, deletion of the bcsA gene, which encodes cellulose synthase, led to a significant increase in cell adhesion to the flask glass walls (Figure 2A); this result is consistent with previous observations suggesting that, although cellulose can promote bacterial adhesion, it can also act as a negative determinant for cell aggregation, particularly in curli-producing E. coli strains [49, 50]. In the C-1a strain, carrying a wild type pnp allele, inactivation of genes involved in biosynthesis of curli, PNAG, cellulose and colanic acid did not result in any notable effects on cell aggregation (Additional file 2: Figure S1).
To establish whether induction of PNAG-dependent cell aggregation in the absence of PNPase is unique to E. coli C-1a or it is conserved in other E. coli strains, we performed adhesion assays comparing the standard laboratory strain MG1655 to its Δpnp derivative KG206. Similar to what observed for the E. coli C strains, deletion of the pnp gene in the MG1655 background resulted in a significant increase in adhesion to solid surfaces, which was totally abolished by pgaA deletion (Additional file 3: Figure S2). However, cell aggregation was not observed in KG206 liquid cultures (data not shown), suggesting that the effect of pnp deletion is less pronounced in the MG1655 background.
Our results clearly indicate that PNAG is required for the aggregative phenotype of pnp mutant strains, suggesting that PNPase may act as a negative regulator of PNAG production. We thus determined by western blotting PNAG relative amounts in both C-1a (WT) and C-5691 (Δpnp) strains using anti-PNAG antibodies. As shown in Figure 3, the Δpnp mutants (both with the single Δpnp mutation and in association with either ΔcsgA or ΔwcaD) exhibited higher PNAG levels relative to the pnp+ strains. As expected, no PNAG could be detected in pgaC mutants, whereas bcsA inactivation, which abolishes cellulose production, led to stimulation of PNAG biosynthesis. Despite increased PNAG production, the pnp+ ΔbcsA strain did not show any detectable cell aggregation (Additional file 2: Figure S1). Discrepancies between PNAG levels and aggregative phenotype in some mutants might be explained by presence of additional adhesion factors, or different timing in PNAG production.
PNPase downregulates pgaABCD operon expression at post-transcriptional level
In E. coli, the functions responsible for PNAG biogenesis are clustered in the pgaABCD operon . By northern blot analysis we found that the pgaABCD transcript was much more abundant in the Δpnp strain than in pnp+ (Figure 4A), suggestive of negative control of pgaABCD transcript stability by PNPase. Increased transcription of the pgaABCD operon was also detected in the E. coli MG1655 Δpnp derivative KG206 (data not shown), in agreement with biofilm formation experiments ( Additional file 3:Figure S2). We investigated the mechanism of pgaABCD regulation by PNPase and its possible connections with known regulatory networks controlling pgaABCD expression. pgaABCD expression is positively regulated at the transcription initiation level by NhaR, while pgaABCD mRNA stability and translation are negatively regulated by the CsrA protein [51, 52]. The 234-nucleotide long pgaABCD 5’-UTR carries multiple binding sites for the translation repressor CsrA . Two small RNAs, CsrB and CsrC, positively regulate pgaABCD by binding CsrA and antagonizing its activity . Stability of the two small RNAs is controlled by CsrD, which triggers RNase E-dependent degradation by a still unknown mechanism . Recently, a third sRNA, McaS, has been involved in this regulatory system as a positive regulator of pgaABCD expression .
Enhanced stability of pgaABCD mRNA may account for (or at least contribute to) the increase in pgaABCD expression. Indeed, RNA degradation kinetics experiments performed by quantitative RT-PCR showed a small, but reproducible 2.5-fold half-life increase of pgaA mRNA in the Δpnp mutant (from 0.6 min in C-1a to 1.5 min in the pnp mutant; Additional file 4: Figure S3). A comparable effect was elicited by deletion of the csrA gene (estimated mRNA half-life, 1.5 min; Additional file 4: Figure S3), known to regulate pgaABCD mRNA stability in E. coli K12 [38, 51].
Post-transcriptional regulation of the pgaABCD operon by the CsrA protein targets its 234 nucleotide-long 5’-UTR. Therefore, we tested whether this determinant was also involved in pgaABCD control by PNPase. To this aim, we constructed several plasmids (see Table 1) harboring both transcriptional and translational fusions between different elements of the pgaABCD regulatory region and the luxAB operon, which encodes the catalytic subunits of Vibrio harveyi luciferase, as a reporter . Luciferase expression in both pnp+ and Δpnp strains was tested using the transcriptional fusion plasmids pΔLpga and pLpga1, which harbor the pgaABCD promoter region (pgaAp) alone (−116 to +32 relative to the transcript start site) and a region encompassing pgaAp and the entire pgaA leader (without its ATG start codon), respectively. In these constructs, translation of the luxAB transcript depends on the vector translation initiation region (TIR). Conversely, pLpga2 carries a translational fusion of the whole 5’-UTR and the first 5 codons of pgaA with luxA. A plasmid expressing luxAB from Ptac promoter (pTLUX) and the vector TIR was also tested as a control of PNPase effects on luciferase mRNA expression. The results of a typical experiment and relative luciferase activity (Δpnp vs. pnp+) are reported in Figure 4B. In agreement with the role of the 5’-UTR as a strong determinant for negative regulation of pgaABCD expression , luciferase activity was much higher in cells carrying the construct lacking the pgaABCD 5’-UTR (pΔLpga) regardless of the presence of PNPase. The small increment in luciferase expression from the pΔLpga plasmid detected in the Δpnp was not due to increased pgaAp promoter activity as it was observed also with pTLUX control plasmid. Conversely, luciferase expression by pLpga1 and pLpga2 was strongly affected by PNPase, as it increased 4.3- and 12.8-fold, respectively, in the PNPase defective strain (Figure 4B). The difference in relative luciferase activity between the pLpga1 and pLpga2 constructs might be explained by higher translation efficiency for the pLpga2 construct in the Δpnp strain. Altogether, the results of luciferase assays (Figure 4B) and mRNA decay experiments (Additional file 4: Figure S3) suggest that PNPase regulates pgaABCD mRNA decay by interacting with cis-acting determinants located in the 5’-UTR. PNPase has been recently shown to play a pivotal role in sRNA stability control [27, 56] and has been involved in degradation of CsrB and CsrC in Salmonella. We hypothesized that PNPase may act as a negative regulator of pgaABCD operon by promoting the degradation of the positive regulators CsrB and/or CsrC . To test this idea, we combined the Δpnp 751 mutation with other deletions of genes either encoding sRNAs known to affect pgaABCD expression (namely, csrB, csrC and mcaS), or csrD, whose gene product favors CsrB and CsrC degradation . We also readily obtained the ΔcsrA::kan mutation in C-1a (pnp+), indicating that, unlike in K-12 strains , csrA is not essential in E. coli C. Conversely, in spite of several attempts performed both by λ Red mediated recombination  and by P1 reciprocal transductions, we could not obtain a Δpnp ΔcsrA double mutant, suggesting that the combination of the two mutations might be lethal.
Each mutant was assayed for the expression of pgaA by quantitative RT-PCR and for PNAG production by western blotting. The results of these analyses showed that, both in the C-1a (pnp+) and in the C-5691 (Δpnp) backgrounds, each tested mutation increased both pgaA mRNA expression (Figure 5A) and PNAG production (Figure 5B). This result was unexpected for mutants lacking CsrB, CsrC or McaS that, according to the current model of pgaABCD regulation, should act as positive regulators of such operon . Thus, while our results support the role of CsrA as a major regulator of pgaABCD expression, they also suggest that the current model for pgaABCD post-transcriptional regulation, which is based on data obtained in E. coli K-12, may not readily apply to E. coli C. The additive effect observed upon combining Δpnp 751 with deletions targeting different sRNAs suggest that PNPase and the sRNAs may act independently on pgaABCD regulation.
In this report, we have shown that PNPase negatively regulates the production of the adhesion factor PNAG, thus maintaining the bacterial cells in a planktonic state (Figures 13) when grown at 37°C in supplemented minimal medium. Our results are in line with previous works by other groups connecting PNPase to regulation of outer membrane proteins in E. coli and curli production in Salmonella . Thus, PNPase seems to play a pivotal role in regulating the composition of cell envelope and the production of adhesion surface determinants. PNPase-dependent regulation of PNAG production requires its ribonuclease activity, as suggested by the observation that overexpression of RNase II can compensate for lack of PNPase (Figure 1B). Cell aggregation in the absence of PNPase is suppressed by RNase II, but not by RNase R. This reminds what previously showed for cold sensitivity in pnp mutants, which is also solely suppressed by RNase II  and reinforces the notion that, albeit partially redundant, RNA degradation pathways possess a certain degree of specificity and are not fully interchangeable .
The precise mechanistic role played by PNPase in regulation of pgaABCD expression, as well as the physiological signals to which it responds, remain elusive. PNPase activity is modulated (at least in vitro) by cyclic-di-GMP , a signal molecule implicated in biofilm formation . However, deletion of the dos gene, encoding a c-di-GMP phosphodiesterase which co-purifies with the RNA degradosome , did not affect pgaABCD expression (data not shown). Key molecules in energy metabolism and carbon flux, such as ATP and citrate also influence PNPase activity [64, 65]. Thus, it can be speculated that environmental or physiological signals might regulate pgaABCD expression by controlling the level of specific metabolites that could directly modulate PNPase activity.
Our data clearly indicate that PNPase controls PNAG production by negatively regulating the pgaABCD operon at post-transcriptional level and that it targets the 5’-UTR of the pgaABCD transcript, thus similar to the translational repressor CsrA (Figures 45 and Additional file 4: Figure S3). This would suggest that the two proteins might belong to the same regulatory network. However, probing this hypothesis is complicated by the observation that in E. coli C, the mechanisms of CsrA-dependent gene expression regulation and its modulation by small RNAs might be more complex than in E. coli K-12, where the current model for CsrA regulation has been developed. This notion is somehow suggested by the fact that, while deletion of the csrA gene is lethal for E. coli K-12 when grown on glucose-based media , this is not the case for E. coli C. Moreover, to our surprise, the lack of putative positive regulators such as CsrB, CsrC and McsA resulted in an increase of pgaABCD expression levels both in the Δpnp and in its parental strain C-1a, which would suggest a negative role of these sRNAs in pgaABCD control (Figure 5). Genes encoding cell surface-associated structures seem to constitute a “hotspot” for post-transcriptional regulation involving small non coding RNAs. For instance, multiple control of gene expression by sRNAs has already been demonstrated for csgD, which encodes the master regulator for the biosynthesis of thin aggregative fimbriae (curli), one of the major adhesion factors in E. coli[28, 55, 66, 67]. It is thus possible that, in E. coli C, increased pgaABCD expression in mutant strains carrying deletions of sRNA-encoding genes might be due to feedback induction of yet unidentified factors which might play a role in CsrA-dependent regulation. This possibility is supported by the observation that CsrB, CsrC and McaS mutually control their transcript level both in E. coli K and C  (T. Carzaniga and F. Briani, unpublished data). pgaABCD operon regulation appears to be an intriguing model system for the study of post-transcriptional modulation of gene expression in bacteria.
In this work, we have unravelled a novel role for PNPase as a negative regulator of pgaABCD expression and PNAG biosynthesis. Thus, PNPase activity contributes to keeping E. coli cells in the planktonic state. Our findings underline the importance of post-transcriptional regulation for genes encoding cell surface-associated structures and factors involved in biofilm formation and suggest the existence of strain-specific variability in these regulatory mechanisms. Indeed, small RNA-dependent post-transcriptional regulation of pgaABCD expression in E. coli C is more complex than the model proposed for E. coli K-12, possibly connected to a central role played by PNAG as a determinant for biofilm formation in the former strain.
Costerton JW: Overview of microbial biofilms. J Ind Microbiol. 1995, 15: 137-140. 10.1007/BF01569816.
Schembri MA, Kjaergaard K, Klemm P: Global gene expression in Escherichia coli biofilms. Mol Microbiol. 2003, 48: 253-267. 10.1046/j.1365-2958.2003.03432.x.
Beloin C, Valle J, Latour-Lambert P, Faure P, Kzreminski M, Balestrino D: Global impact of mature biofilm lifestyle on Escherichia coli K-12 gene expression. Mol Microbiol. 2004, 51: 659-674.
Shapiro JA: Thinking about bacterial populations as multicellular organisms. Annu Rev Microbiol. 1998, 52: 81-104. 10.1146/annurev.micro.52.1.81.
Allesen-Holm M, Barken KB, Yang L, Klausen M, Webb JS, Kjelleberg S: A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms. Mol Microbiol. 2006, 59: 1114-1128. 10.1111/j.1365-2958.2005.05008.x.
White AP, Surette MG: Comparative genetics of the rdar morphotype in Salmonella. J Bacteriol. 2006, 188: 8395-8406. 10.1128/JB.00798-06.
Hughes KA, Sutherland IW, Jones MV: Biofilm susceptibility to bacteriophage attack: the role of phage-borne polysaccharide depolymerase. Microbiology. 1998, 144: 3039-3047. 10.1099/00221287-144-11-3039.
Merritt JH, Brothers KM, Kuchma SL, O'Toole GA: SadC reciprocally influences biofilm formation and swarming motility via modulation of exopolysaccharide production and flagellar function. J Bacteriol. 2007, 189: 8154-8164. 10.1128/JB.00585-07.
Pehl MJ, Jamieson WD, Kong K, Forbester JL, Fredendall RJ, Gregory GA: Genes that influence swarming motility and biofilm formation in Variovorax paradoxus EPS. PLoS One. 2012, 7: e31832-10.1371/journal.pone.0031832.
Romling U, Rohde M, Olsen A, Normark S, Reinkoster J: AgfD, the checkpoint of multicellular and aggregative behaviour in Salmonella typhimurium regulates at least two independent pathways. Mol Microbiol. 2000, 36: 10-23. 10.1046/j.1365-2958.2000.01822.x.
Gerstel U, Park C, Romling U: Complex regulation of csgD promoter activity by global regulatory proteins. Mol Microbiol. 2003, 49: 639-654.
Gjermansen M, Ragas P, Sternberg C, Molin S, Tolker-Nielsen T: Characterization of starvation-induced dispersion in Pseudomonas putida biofilms. Environ Microbiol. 2005, 7: 894-906. 10.1111/j.1462-2920.2005.00775.x.
Karatan E, Watnick P: Signals, regulatory networks, and materials that build and break bacterial biofilms. Microbiol Mol Biol Rev. 2009, 73: 310-347. 10.1128/MMBR.00041-08.
Haugo AJ, Watnick PI: Vibrio cholerae CytR is a repressor of biofilm development. Mol Microbiol. 2002, 45: 471-483. 10.1046/j.1365-2958.2002.03023.x.
Irie Y, Starkey M, Edwards AN, Wozniak DJ, Romeo T, Parsek MR: Pseudomonas aeruginosa biofilm matrix polysaccharide Psl is regulated transcriptionally by RpoS and post-transcriptionally by RsmA. Mol Microbiol. 2010, 78: 158-172.
Ross P, Mayer R, Benziman M: Cellulose biosynthesis and function in bacteria. Microbiol Rev. 1991, 55: 35-58.
Simm R, Morr M, Kader A, Nimtz M, Romling U: GGDEF and EAL domains inversely regulate cyclic di-GMP levels and transition from sessility to motility. Mol Microbiol. 2004, 53: 1123-1134. 10.1111/j.1365-2958.2004.04206.x.
Schirmer T, Jenal U: Structural and mechanistic determinants of c-di-GMP signalling. Nat Rev Microbiol. 2009, 7: 724-735. 10.1038/nrmicro2203.
Mohanty BK, Kushner SR: Polynucleotide phosphorylase, RNase II and RNase E play different roles in the in vivo modulation of polyadenylation in Escherichia coli. Mol Microbiol. 2000, 36: 982-994. 10.1046/j.1365-2958.2000.01921.x.
Mohanty BK, Kushner SR: The majority of Escherichia coli mRNAs undergo post-transcriptional modification in exponentially growing cells. Nucleic Acids Res. 2006, 34: 5695-5704. 10.1093/nar/gkl684.
Carpousis AJ, Van Houwe G, Ehretsmann C, Krisch HM: Copurification of E. coli RNAase E and PNPase: evidence for a specific association between two enzymes important in RNA processing and degradation. Cell. 1994, 76: 889-900. 10.1016/0092-8674(94)90363-8.
Miczak A, Kaberdin VR, Wei CL, Lin-Chao S: Proteins associated with RNase E in a multicomponent ribonucleolytic complex. Proc Natl Acad Sci USA. 1996, 93: 3865-3869. 10.1073/pnas.93.9.3865.
Cardenas PP, Carzaniga T, Zangrossi S, Briani F, Garcia-Tirado E, Deho G: Polynucleotide phosphorylase exonuclease and polymerase activities on single-stranded DNA ends are modulated by RecN, SsbA and RecA proteins. Nucleic Acids Res. 2011, 39: 9250-9261. 10.1093/nar/gkr635.
Rath D, Mangoli SH, Pagedar AR, Jawali N: Involvement of pnp in survival of UV radiation in Escherichia coli K-12. Microbiology. 2012, 158: 1196-1205. 10.1099/mic.0.056309-0.
Zangrossi S, Briani F, Ghisotti D, Regonesi ME, Tortora P, Dehò G: Transcriptional and post-transcriptional control of polynucleotide phosphorylase during cold acclimation in Escherichia coli. Mol Microbiol. 2000, 36: 1470-1480.
Piazza F, Zappone M, Sana M, Briani F, Dehò G: Polynucleotide phosphorylase of Escherichia coli is required for the establishment of bacteriophage P4 immunity. J Bacteriol. 1996, 178: 5513-5521.
De Lay N, Gottesman S: Role of polynucleotide phosphorylase in sRNA function in Escherichia coli. RNA. 2011, 17: 1172-1189. 10.1261/rna.2531211.
Boehm A, Vogel J: The csgD mRNA as a hub for signal integration via multiple small RNAs. Mol Microbiol. 2012, 84: 1-5. 10.1111/j.1365-2958.2012.08033.x.
Bertani G, Weigle JJ: Host controlled variation in bacterial viruses. J Bacteriol. 1953, 65: 113-121.
Daniel AS, Fuller-Pace FV, Legge DM, Murray NE: Distribution and diversity of hsd genes in Escherichia coli and other enteric bacteria. J Bacteriol. 1988, 170: 1775-1782.
Jensen KF: The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression. J Bacteriol. 1993, 175: 3401-3407.
Datsenko KA, Wanner BL: One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA. 2000, 97: 6640-6645. 10.1073/pnas.120163297.
Gualdi L, Tagliabue L, Landini P: Biofilm formation-gene expression relay system in Escherichia coli: modulation of sigmaS-dependent gene expression by the CsgD regulatory protein via sigmaS protein stabilization. J Bacteriol. 2007, 189: 8034-8043. 10.1128/JB.00900-07.
Dehò G, Zangrossi S, Sabbattini P, Sironi G, Ghisotti D: Bacteriophage P4 immunity controlled by small RNAs via transcription termination. Mol Microbiol. 1992, 6: 3415-3425. 10.1111/j.1365-2958.1992.tb02209.x.
Briani F, Del Favero M, Capizzuto R, Consonni C, Zangrossi S, Greco C: Genetic analysis of polynucleotide phosphorylase structure and functions. Biochimie. 2007, 89: 145-157. 10.1016/j.biochi.2006.09.020.
Briani F, Curti S, Rossi F, Carzaniga T, Mauri P, Dehò G: Polynucleotide phosphorylase hinders mRNA degradation upon ribosomal protein S1 overexpression in Escherichia coli. RNA. 2008, 14: 2417-2429. 10.1261/rna.1123908.
Jaspers MC, Suske WA, Schmid A, Goslings DA, Kohler HP, Der Meer v: HbpR, a new member of the XylR/DmpR subclass within the NtrC family of bacterial transcriptional activators, regulates expression of 2-hydroxybiphenyl metabolism in Pseudomonas azelaica HBP1. J Bacteriol. 2000, 182: 405-417. 10.1128/JB.182.2.405-417.2000.
Cerca N, Jefferson KK: Effect of growth conditions on poly-N-acetylglucosamine expression and biofilm formation in Escherichia coli. FEMS Microbiol Lett. 2008, 283: 36-41. 10.1111/j.1574-6968.2008.01142.x.
Maira-Litran T, Kropec A, Abeygunawardana C, Joyce J, Mark G, Goldmann DA: Immunochemical properties of the staphylococcal poly-N-acetylglucosamine surface polysaccharide. Infect Immun. 2002, 70: 4433-4440. 10.1128/IAI.70.8.4433-4440.2002.
Sasaki I, Bertani G: Growth abnormalities in Hfr derivatives of Escherichia coli strain C. J Gen Microbiol. 1965, 40: 365-376.
Regonesi ME, Del Favero M, Basilico F, Briani F, Benazzi L, Tortora P: Analysis of the Escherichia coli RNA degradosome composition by a proteomic approach. Biochimie. 2006, 88: 151-161. 10.1016/j.biochi.2005.07.012.
Olsen A, Jonsson A, Normark S: Fibronectin binding mediated by a novel class of surface organelles on Escherichia coli. Nature. 1989, 338: 652-655. 10.1038/338652a0.
Romling U, Bian Z, Hammar M, Sierralta WD, Normark S: Curli fibers are highly conserved between Salmonella typhimurium and Escherichia coli with respect to operon structure and regulation. J Bacteriol. 1998, 180: 722-731.
Perry RD, Pendrak ML, Schuetze P: Identification and cloning of a hemin storage locus involved in the pigmentation phenotype of Yersinia pestis. J Bacteriol. 1990, 172: 5929-5937.
Nucleo E, Steffanoni L, Fugazza G, Migliavacca R, Giacobone E, Navarra A: Growth in glucose-based medium and exposure to subinhibitory concentrations of imipenem induce biofilm formation in a multidrug-resistant clinical isolate of Acinetobacter baumannii. BMC Microbiol. 2009, 9: 270-10.1186/1471-2180-9-270.
Prigent-Combaret C, Prensier G, Le Thi TT, Vidal O, Lejeune P, Dorel C: Developmental pathway for biofilm formation in curli-producing Escherichia coli strains: role of flagella, curli and colanic acid. Environ Microbiol. 2000, 2: 450-464. 10.1046/j.1462-2920.2000.00128.x.
May T, Okabe S: Escherichia coli harboring a natural IncF conjugative F plasmid develops complex mature biofilms by stimulating synthesis of colanic acid and curli. J Bacteriol. 2008, 190: 7479-7490. 10.1128/JB.00823-08.
Wang X, Preston JF, Romeo T: The pgaABCD locus of Escherichia coli promotes the synthesis of a polysaccharide adhesin required for biofilm formation. J Bacteriol. 2004, 186: 2724-2734. 10.1128/JB.186.9.2724-2734.2004.
Gualdi L, Tagliabue L, Bertagnoli S, Ierano T, De Castro C, Landini P: Cellulose modulates biofilm formation by counteracting curli-mediated colonization of solid surfaces in Escherichia coli. Microbiology. 2008, 154: 2017-2024. 10.1099/mic.0.2008/018093-0.
Ma Q, Wood TK: OmpA influences Escherichia coli biofilm formation by repressing cellulose production through the CpxRA two-component system. Environ Microbiol. 2009, 11: 2735-2746. 10.1111/j.1462-2920.2009.02000.x.
Wang X, Dubey AK, Suzuki K, Baker CS, Babitzke P, Romeo T: CsrA post-transcriptionally represses pgaABCD, responsible for synthesis of a biofilm polysaccharide adhesin of Escherichia coli. Mol Microbiol. 2005, 56: 1648-1663. 10.1111/j.1365-2958.2005.04648.x.
Goller C, Wang X, Itoh Y, Romeo T: The cation-responsive protein NhaR of Escherichia coli activates pgaABCD transcription, required for production of the biofilm adhesin poly-beta-1,6-N-acetyl-D-glucosamine. J Bacteriol. 2006, 188: 8022-8032. 10.1128/JB.01106-06.
Weilbacher T, Suzuki K, Dubey AK, Wang X, Gudapaty S, Morozov I: A novel sRNA component of the carbon storage regulatory system of Escherichia coli. Mol Microbiol. 2003, 48: 657-670. 10.1046/j.1365-2958.2003.03459.x.
Suzuki K, Babitzke P, Kushner SR, Romeo T: Identification of a novel regulatory protein (CsrD) that targets the global regulatory RNAs CsrB and CsrC for degradation by RNase E. Genes Dev. 2006, 20: 2605-2617. 10.1101/gad.1461606.
Thomason MK, Fontaine F, De Lay N, Storz G: A small RNA that regulates motility and biofilm formation in response to changes in nutrient availability in Escherichia coli. Mol Microbiol. 2012, 84: 17-35. 10.1111/j.1365-2958.2012.07965.x.
Andrade JM, Pobre V, Matos AM, Arraiano CM: The crucial role of PNPase in the degradation of small RNAs that are not associated with Hfq. RNA. 2012, 18: 844-855. 10.1261/rna.029413.111.
Viegas SC, Pfeiffer V, Sittka A, Silva IJ, Vogel J, Arraiano CM: Characterization of the role of ribonucleases in Salmonella small RNA decay. Nucleic Acids Res. 2007, 35: 7651-7664. 10.1093/nar/gkm916.
Timmermans J, Van Melderen L: Conditional essentiality of the csrA gene in Escherichia coli. J Bacteriol. 2009, 191: 1722-1724. 10.1128/JB.01573-08.
Andrade JM, Arraiano CM: PNPase is a key player in the regulation of small RNAs that control the expression of outer membrane proteins. Rna-A Publication of the Rna Society. 2008, 14: 543-551. 10.1261/rna.683308.
Rouf SF, Ahmad I, Anwar N, Vodnala SK, Kader A, Romling U: Opposing contributions of polynucleotide phosphorylase and the membrane protein NlpI to biofilm formation by Salmonella enterica serovar Typhimurium. J Bacteriol. 2011, 193: 580-582. 10.1128/JB.00905-10.
Awano N, Inouye M, Phadtare S: RNase activity of polynucleotide phosphorylase is critical at low temperature in Escherichia coli and is complemented by RNase II. J Bacteriol. 2008, 190: 5924-5933. 10.1128/JB.00500-08.
Mohanty BK, Kushner SR: Genomic analysis in Escherichia coli demonstrates differential roles for polynucleotide phosphorylase and RNase II in mRNA abundance and decay. Mol Microbiol. 2003, 50: 645-658. 10.1046/j.1365-2958.2003.03724.x.
Tuckerman JR, Gonzalez G, Gilles-Gonzalez MA: Cyclic di-GMP activation of polynucleotide phosphorylase signal-dependent RNA processing. J Mol Biol. 2011, 407: 633-639. 10.1016/j.jmb.2011.02.019.
Del Favero M, Mazzantini E, Briani F, Zangrossi S, Tortora P, Deho G: Regulation of Escherichia coli polynucleotide phosphorylase by ATP. J Biol Chem. 2008, 283: 27355-27359. 10.1074/jbc.C800113200.
Nurmohamed S, Vincent HA, Titman CM, Chandran V, Pears MR, Du D: Polynucleotide phosphorylase activity may be modulated by metabolites in Escherichia coli. J Biol Chem. 2011, 286: 14315-14323. 10.1074/jbc.M110.200741.
Jorgensen MG, Nielsen JS, Boysen A, Franch T, Moller-Jensen J, Valentin-Hansen P: Small regulatory RNAs control the multi-cellular adhesive lifestyle of Escherichia coli. Mol Microbiol. 2012, 84: 36-50. 10.1111/j.1365-2958.2012.07976.x.
Mika F, Busse S, Possling A, Berkholz J, Tschowri N, Sommerfeldt N: Targeting of csgD by the small regulatory RNA RprA links stationary phase, biofilm formation and cell envelope stress in Escherichia coli. Mol Microbiol. 2012, 84: 51-65. 10.1111/j.1365-2958.2012.08002.x.
Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M: Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. 2006, 2006: 2-
Tagliabue L, Antoniani D, Maciag A, Bocci P, Raffaelli N, Landini P: The diguanylate cyclase YddV controls production of the exopolysaccharide poly-N-acetylglucosamine (PNAG) through regulation of the PNAG biosynthetic pgaABCD operon. Microbiology. 2010, 156: 2901-2911. 10.1099/mic.0.041350-0.
Guzman LM, Belin D, Carson MJ, Beckwith J: Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995, 177: 4121-4130.
Ghetta A, Matus-Ortega M, Garcia-Mena J, Dehò G, Tortora P, Regonesi ME: Polynucleotide phosphorylase-based photometric assay for inorganic phosphate. Anal Biochem. 2004, 327: 209-214. 10.1016/j.ab.2004.01.034.
Cairrao F, Chora A, Zilhao R, Carpousis AJ, Arraiano CM: RNase II levels change according to the growth conditions: characterization of gmr, a new Escherichia coli gene involved in the modulation of RNase II. Mol Microbiol. 2001, 39: 1550-1561. 10.1046/j.1365-2958.2001.02342.x.
Lessl M, Balzer D, Lurz R, Waters VL, Guiney DG, Lanka E: Dissection of IncP conjugative plasmid transfer: definition of the transfer region Tra2 by mobilization of the Tra1 region in trans. J Bacteriol. 1992, 174: 2493-2500.
Wall JD, Harriman PD: Phage P1 mutants with altered transducing abilities for Escherichia coli. Virology. 1974, 59: 532-544. 10.1016/0042-6822(74)90463-2.
We thank Gerald B. Pier (Harvard Medical School, Boston, USA) for his kind gift of anti-PNAG antibodies, Cecilia Arraiano for sending pFCT6.9 plasmid, Maria Pasini for the microscope images, Michela Casali for technical assistance and Michela Gambino for the statistical analysis. This study was supported by PRIN (Project 2008K37RHP) Research Programs of the Italian Ministry for University and Research.
FB, GD and PL conceived the project and designed the experiments. FB and PL wrote the manuscript. TC and DA designed and performed the experiments. All authors read and approved the final manuscript.
Thomas Carzaniga, Davide Antoniani contributed equally to this work.
Electronic supplementary material
Additional file 2: Figure S1: Effects of inactivation of genes encoding adhesion factors and biofilm determinants in the C-1a strain. C-1a (pnp+) and its derivatives carrying mutations in genes encoding for adhesion determinants (ΔpgaC, impaired in PNAG production; ΔbcsA, impaired in cellulose production; ΔcsgA, impaired in curli production; ΔwcaD, impaired in colanic acid production) were grown over night in M9Glu/sup at 37°C in glass flasks. Cell aggregates were stained with crystal violet. (PPTX 369 KB)
Additional file 3: Figure S2: Surface adhesion of pnp deletion mutant derivative of E. coli MG1655 and identification of the adhesion factor involved. Surface adhesion to polystyrene microtiter plates by MG1655 (pnp+), KG206 (Δpnp), and KG206 derivatives carrying mutations in genes coding for adhesion determinants (ΔpgaA, AM56; ΔbcsA, AM72; ΔcsgA, AM70; ΔwcaD, AM105) was assessed at 37°C in M9Glu/sup. Adhesion unit values, assessed as previously described , are the average of three independent experiments and standard deviation is shown. The overall p-value obtained by ANOVA is indicated in the graph. Letters provide the representation for posthoc comparisons. According to posthoc analysis (Tukey’s HSD, p < 0.05), means sharing the same letter are not significantly different from each other. (PPTX 46 KB)
Additional file 4: Figure S3: pgaA mRNA decay analysis. Bacterial cultures of C-1a (pnp+), C-5691 (Δpnp) and C-5938 (ΔcsrA) were grown up to OD600 = 0.8 in M9Glu/sup, rifampicin (final concentration of 0.4 mg/ml) was added, and samples for RNA extraction were taken at different time points immediately before (t = 0) and after antibiotic addition. pgaA mRNA degradation kinetics was estimated by quantitative RT-PCR with oligonucleotides PL99 and PL100, as detailed in Methods. (PPTX 51 KB)
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Carzaniga, T., Antoniani, D., Dehò, G. et al. The RNA processing enzyme polynucleotide phosphorylase negatively controls biofilm formation by repressing poly-N-acetylglucosamine (PNAG) production in Escherichia coli C. BMC Microbiol 12, 270 (2012). https://doi.org/10.1186/1471-2180-12-270