Regulation of phenylacetic acid uptake is σ54 dependent in Pseudomonas putidaCA-3

BMC Microbiology

Table 2 Primers for PCR amplifications.

Primer	Sequence 5'-3'	Annealing temp°C
GS326	acgatgcccagggagtagaga	60
OP2-55	gctgatggcgatgaatgaaca	55
TNInt2	cctgcaggcatgcaagcttcggc	65
27F	agagtttgatcatggctcag	55
1492R	ggttaccttgttacgactt	55
paaFf paaFr paaGf	ggttgagcatgtaggacggt gccaataccgccttgcttga ccgaaggcaactgggtcac	57 57 55
paaGr	aggcggcgttcttgttctg	55
paaLf	cggcatgctcgcgaccacctg	60
paaLr	aaagcgatgttctgcgactc	60
Sig54f-Hind	tattacaagcttatgaaaccatcgctgtcctaaaaatg^a	60
Sig54r-Xba	atcatttctagactacatcagtcgcttgcgttcgctcga^b	60
paaLproF	gccgcgcaacagccagagc	63
paaLproR	cgccgagatgccgaggaagg	63
paaLf-Hind	tattacaagcttatgacagccctgcgctccttcacctt^a	60
paaLr-Xba	atcatttctagactagtggttactggccttggct^b	60

a: Hind III restriction site, b: Xba I restriction site.
Oligonucleotide sequences and annealing temperatures utilised in polymerase chain reaction amplification of gene targets from P. putida CA-3 in this study.

ISSN: 1471-2180