Figure 3
From: Regulation of phenylacetic acid uptake is σ54 dependent in Pseudomonas putidaCA-3
PaCoA Catabolon gene transcription analyses. Reverse transcription polymerase chain reaction analysis of P. putida CA-3 parent (WT) and rpoN disrupted mutant (D7) strains, following growth of cultures on styrene (sty), citrate (cit) and phenylacetic acid (PAA), respectively. 16S rRNA amplification acted as a positive control. The paaL, paaF and paaG, gene targets (indicated on the left hand side) were selected as representative genes of the operons for phenylacetic uptake, β-oxidation and ring hydroxylation, respectively.