A Blue Native-PAGE analysis of membrane protein complexes in Clostridium thermocellum

Peng, Yanfeng; Luo, Yuanming; Yu, Tingting; Xu, Xinping; Fan, Keqiang; Zhao, Youbao; Yang, Keqian

doi:10.1186/1471-2180-11-22

BMC Microbiology

Table 1 Putative membrane proteins of C. thermocellum identified by mass spectrometry

From: A Blue Native-PAGE analysis of membrane protein complexes in Clostridium thermocellum

Spots Id^a	Gene locus	NCBI accession number/gi	Protein descriptions^b	Mr^c	MASCOT Score	Peptides matched	Sequence Coverage (%)	TMHMM prediction
1	Cthe_0423	125972944	bifunctional acetaldehyde/alcohol dehydrogenase	95992.3	427	46	39
2	Cthe_0858	125713600	hypothetical protein	35296.4	411	26	58	1
3	Cthe_2253	125974738	ATP-dependent metalloprotease FtsH	66652.9	253	34	45	2
4	Cthe_0699	125713442	carboxyl transferase	56037.9	700	39	49
5	Cthe_1020	125973535	solute-binding protein	49976.2	164	28	45
6	Cthe_0016	125972541	Ferritin and Dps	18602.9	61	9	42
7	Cthe_0016	125972541	Ferritin and Dps	18602.9	189	14	42
8	Cthe_2693	125975175	hypothetical protein	17817.5	74	12	26	1
9	Cthe_2267	125714977	V-type ATP synthase subunit A	65320	214	32	33
10	Cthe_1020	125973535	solute-binding protein	49976.2	199	25	44
10	Cthe_2268	125714978	V-type ATP synthase beta chain	50714.2	109	26	43
10	Cthe_2608	125975091	ATP synthase F1, beta subunit	51000	87	22	38
11	Cthe_2606	125975089	ATP synthase F1, alpha subunit	55810	307	22	33
12	Cthe_2348	125715058	S-layer-like region; Ig-related	113309.3	550	42	34	1
13	Cthe_0418	125972939	polynucleotide phosphorylase/polyadenylase	77304	84	17	26
14	Cthe_3148	125975626	ABC transporter related protein	70461.1	95	12	16	5
15	Cthe_0699	125973217	carboxyl transferase	56037.9	148	25	38
16	Cthe_1020	125973535	solute-binding protein	49976.2	486	33	48
17	Cthe_1557	125974066	ABC transporter related protein ATP-binding protein	30203.7	175	21	47
18	Cthe_1018	125973533	binding-protein-dependent transport systems inner membrane component	31919.9	67	13	23	6
19	Cthe_1840	125974344	cysteine synthase	33392	469	25	57
20	Cthe_1104	125713844	prepilin-type cleavage/methylation	19233.2	183	21	65
21	Cthe_1862	125974366	ABC transporter related protein	42056.4	317	31	38
22	Cthe_1754	125714483	solute-binding protein	35734.5	143	19	48	1
23	Cthe_2709	125975191	hypothetical protein	55140	95	14	19
24	Cthe_1020	125973535	solute-binding protein	49976.2	385	32	47
25	Cthe_1754	125714483	solute-binding protein	35734.5	241	29	64	1
26	Cthe_1555	125974064	ABC-type metal ion transport system periplasmic component	32242.5	73	12	32	1
27	Cthe_1869	125714598	ornithine carbamoyltransferase	34235.9	304	20	47
28	Cthe_1104	125713844	prepilin-type cleavage/methylation	19233.2	539	21	68

Note:
^aSpots identification numbers (Spots ID) correspond to the numbers in Figure 1.
^bProtein annotations are based on the genome annotation of C. thermocellum ATCC 27405.
^cMr, molecular mass.

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ISSN: 1471-2180

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