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Table 1 Putative membrane proteins of C. thermocellum identified by mass spectrometry

From: A Blue Native-PAGE analysis of membrane protein complexes in Clostridium thermocellum

Spots Ida Gene locus NCBI accession number/gi Protein descriptionsb Mrc MASCOT Score Peptides matched Sequence Coverage (%) TMHMM prediction
1 Cthe_0423 125972944 bifunctional acetaldehyde/alcohol dehydrogenase 95992.3 427 46 39  
2 Cthe_0858 125713600 hypothetical protein 35296.4 411 26 58 1
3 Cthe_2253 125974738 ATP-dependent metalloprotease FtsH 66652.9 253 34 45 2
4 Cthe_0699 125713442 carboxyl transferase 56037.9 700 39 49  
5 Cthe_1020 125973535 solute-binding protein 49976.2 164 28 45  
6 Cthe_0016 125972541 Ferritin and Dps 18602.9 61 9 42  
7 Cthe_0016 125972541 Ferritin and Dps 18602.9 189 14 42  
8 Cthe_2693 125975175 hypothetical protein 17817.5 74 12 26 1
9 Cthe_2267 125714977 V-type ATP synthase subunit A 65320 214 32 33  
10 Cthe_1020 125973535 solute-binding protein 49976.2 199 25 44  
10 Cthe_2268 125714978 V-type ATP synthase beta chain 50714.2 109 26 43  
10 Cthe_2608 125975091 ATP synthase F1, beta subunit 51000 87 22 38  
11 Cthe_2606 125975089 ATP synthase F1, alpha subunit 55810 307 22 33  
12 Cthe_2348 125715058 S-layer-like region; Ig-related 113309.3 550 42 34 1
13 Cthe_0418 125972939 polynucleotide phosphorylase/polyadenylase 77304 84 17 26  
14 Cthe_3148 125975626 ABC transporter related protein 70461.1 95 12 16 5
15 Cthe_0699 125973217 carboxyl transferase 56037.9 148 25 38  
16 Cthe_1020 125973535 solute-binding protein 49976.2 486 33 48  
17 Cthe_1557 125974066 ABC transporter related protein ATP-binding protein 30203.7 175 21 47  
18 Cthe_1018 125973533 binding-protein-dependent transport systems inner membrane component 31919.9 67 13 23 6
19 Cthe_1840 125974344 cysteine synthase 33392 469 25 57  
20 Cthe_1104 125713844 prepilin-type cleavage/methylation 19233.2 183 21 65  
21 Cthe_1862 125974366 ABC transporter related protein 42056.4 317 31 38  
22 Cthe_1754 125714483 solute-binding protein 35734.5 143 19 48 1
23 Cthe_2709 125975191 hypothetical protein 55140 95 14 19  
24 Cthe_1020 125973535 solute-binding protein 49976.2 385 32 47  
25 Cthe_1754 125714483 solute-binding protein 35734.5 241 29 64 1
26 Cthe_1555 125974064 ABC-type metal ion transport system periplasmic component 32242.5 73 12 32 1
27 Cthe_1869 125714598 ornithine carbamoyltransferase 34235.9 304 20 47  
28 Cthe_1104 125713844 prepilin-type cleavage/methylation 19233.2 539 21 68  
  1. Note:
  2. aSpots identification numbers (Spots ID) correspond to the numbers in Figure 1.
  3. bProtein annotations are based on the genome annotation of C. thermocellum ATCC 27405.
  4. cMr, molecular mass.