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Table 1 Primers used and conditions for denaturing gradient gel electrophoresis (DGGE) analysis

From: Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization

Primer Primer sequence (5'-3') Amplicon size (bp) Annealing temperature (°C) DGGE gradient (%) Target group Reference
V6-V8: F968-GC
V6-V8: R1401
GC clampa-AACGCGAAGAACCT
CGGTGTGTACAAGACCC
489 55 45-55 (feces)
40-65 (biopsies)
Eubacteria This study
g- Bifid F
g-Bifid R-GC
CTCCTGGAAACGGGTGG
GC clampa-GGTGTTCTTCCCGATATCTACA
596 65 45-60 Bifidobacterium This study
Lac1
Lac2GC
AGCAGTAGGGAATCTTCCA
GC clampa - ATTYCACCGCTACACATG
380 61 35-50 (feces)
35-70 (biopsies)
Lactobacillus groupb [24]
Bif164-f
Bif662-GC-r
GGGTGGTAATGCCGGATG
GC clamp a- CCACCGTTACACCGGGAA
520 62 45-55 Bifidobacterium [47]
Bif164-GC-f
Bif662-r
GC clamp a - GGGTGGTAATGCCGGATG
CCACCGTTACACCGGGAA
520 62 45-55 Bifidobacterium [47]
  1. aGC clamp sequence: CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCC.
  2. bLactobacillus group comprises the genera Lactobacillus, Leuconostoc, Pediococcus and Weisella.