Table 1 Primers used and conditions for denaturing gradient gel electrophoresis (DGGE) analysis
Primer | Primer sequence (5'-3') | Amplicon size (bp) | Annealing temperature (°C) | DGGE gradient (%) | Target group | Reference |
|---|---|---|---|---|---|---|
V6-V8: F968-GC V6-V8: R1401 | GC clampa-AACGCGAAGAACCT CGGTGTGTACAAGACCC | 489 | 55 | 45-55 (feces) 40-65 (biopsies) | Eubacteria | This study |
g- Bifid F g-Bifid R-GC | CTCCTGGAAACGGGTGG GC clampa-GGTGTTCTTCCCGATATCTACA | 596 | 65 | 45-60 | Bifidobacterium | This study |
Lac1 Lac2GC | AGCAGTAGGGAATCTTCCA GC clampa - ATTYCACCGCTACACATG | 380 | 61 | 35-50 (feces) 35-70 (biopsies) | Lactobacillus groupb | [24] |
Bif164-f Bif662-GC-r | GGGTGGTAATGCCGGATG GC clamp a- CCACCGTTACACCGGGAA | 520 | 62 | 45-55 | Bifidobacterium | [47] |
Bif164-GC-f Bif662-r | GC clamp a - GGGTGGTAATGCCGGATG CCACCGTTACACCGGGAA | 520 | 62 | 45-55 | Bifidobacterium | [47] |