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Table 1 Primers for amplifying epitopes of OmpL1 and LipL41

From: Characterization of Conserved Combined T and B Cell Epitopes in Leptospira interrogansMajor Outer Membrane Proteins OmpL1 and LipL41

Protein Location Primer Sequence(5'-3')
OmpL1 59-78 O1-F59 cgGGTACCTTTCTATTCTCACTCTgttcgatcgtccaatacctg
   O1-R59 ttCGGCCGagccgcctgggttttgaaaacaagcag
  87-98 O1-F87 cgGGTACCTTTCTATTCTCACTCTtatataggagttgctcctag
   O1-R87 ttCGGCCGagccgccagcaggaatcgcttttctag
  173-191 O1-F173 cgGGTACCTTTCTATTCTCACTCTagttctatcgtcattcctgc
   O1-R173 ttCGGCCGagccgccagcgtcttcagtaacattc
  297-320 O1-F297 cgGGTACCTTTCTATTCTCACTCTctttctccttttccagc
   O1-R297 ttCGGCCGagccgccgagttcgtgtttataaccg
LipL41 30-48 L41-F30 cgGGTACCTTTCTATTCTCACTCTgtattcccgaaagataaaga
   L41-R30 ttCGGCCGagccgccacgaatggttccgaggaat
  181-195 L41-F181 cgGGTACCTTTCTATTCTCACTCTgtacgtatgatgttaattc
   L41-R181 ttCGGCCGagccgcctactttaatgagagtagc
  233-256 L41-F233 cgGGTACCTTTCTATTCTCACTCTgaagctgcttatatc
   L41-R233 ttCGGCCGagccgcctttaacgaaaactttaattc
  263-282 L41-F263 cgGGTACCTTTCTATTCTCACTCTaaagaacttcttcaagaaggtt
   L41-R263 ttCGGCCGagccgccttttttgaaacttggagtttc
  1. EcoR52 I and Kpn I sites are capital and underlined. Sequence encoding M13KE leader peptide is capitalized. The sequences in bold encode flexible peptide.