A bacterial PriB with weak single-stranded DNA binding activity can stimulate the DNA unwinding activity of its cognate PriA helicase
BMC Microbiology volume 11, Article number: 189 (2011)
Bacterial DNA replication restart pathways facilitate reinitiation of DNA replication following disruptive encounters of a replisome with DNA damage, thereby allowing complete and faithful duplication of the genome. In Neisseria gonorrhoeae, the primosome proteins that catalyze DNA replication restart differ from the well-studied primosome proteins of E. coli with respect to the number of proteins involved and the affinities of their physical interactions: the PriA:PriB interaction is weak in E. coli, but strong in N. gonorrhoeae, and the PriB:DNA interaction is strong in E. coli, but weak in N. gonorrhoeae. In this study, we investigated the functional consequences of this affinity reversal.
We report that N. gonorrhoeae PriA's DNA binding and unwinding activities are similar to those of E. coli PriA, and N. gonorrhoeae PriA's helicase activity is stimulated by its cognate PriB, as it is in E. coli. This finding is significant because N. gonorrhoeae PriB's single-stranded DNA binding activity is weak relative to that of E. coli PriB, and in E. coli, PriB's single-stranded DNA binding activity is important for PriB stimulation of PriA helicase. Furthermore, a N. gonorrhoeae PriB variant defective for binding single-stranded DNA can stimulate PriA's helicase activity, suggesting that DNA binding by PriB might not be important for PriB stimulation of PriA helicase in N. gonorrhoeae. We also demonstrate that N. gonorrhoeae PriB stimulates ATP hydrolysis catalyzed by its cognate PriA. This activity of PriB has not been observed in E. coli, and could be important for PriB stimulation of PriA helicase in N. gonorrhoeae.
The results of this study demonstrate that a bacterial PriB homolog with weak single-stranded DNA binding activity can stimulate the DNA unwinding activity of its cognate PriA helicase. While it remains unclear if N. gonorrhoeae PriB's weak DNA binding activity is required for PriB stimulation of PriA helicase, the ability of PriB to stimulate PriA-catalyzed ATP hydrolysis could play an important role. Thus, the weak interaction between N. gonorrhoeae PriB and DNA might be compensated for by the strong interaction between PriB and PriA, which could result in allosteric activation of PriA's ATPase activity.
DNA damage contributes to genome instability by creating barriers that hinder the progression of the replication machinery (replisome) during DNA replication . Consequently, DNA replication forks that stall or collapse due to encounters of the replisome with DNA damage must be reactivated to allow complete replication of the genome and ensure survival of the cell. DNA replication restart pathways provide bacterial cells with a mechanism to reactivate replisomes that are disrupted in this manner . Catalyzed by primosome proteins such as PriA, PriB, PriC, DnaT, and DnaG, DNA replication restart pathways facilitate origin-independent reloading of the replicative helicase onto a repaired DNA replication fork in a process that involves coordinated protein and nucleic acid binding within a nucleoprotein complex called the DNA replication restart primosome .
DNA replication restart initiated by PriA helicase is a highly coordinated process and probably represents the major pathway of DNA replication restart in E. coli. PriA belongs to the DExH family of DNA helicases and is well-conserved among sequenced bacterial genomes . PriA is thought to recognize and bind to repaired DNA replication forks and D-loop recombination intermediates, facilitate assembly of the primosome complex by recruiting other primosome proteins, and catalyze duplex DNA unwinding using energy furnished by hydrolysis of ATP [4, 5]. Recruitment of PriB to a PriA:DNA complex stabilizes PriA on the DNA  and enhances its helicase activity through a mechanism that involves PriB's single-stranded DNA-binding activity . Formation of a PriA:PriB:DNA complex leads to recruitment of DnaT, perhaps through physical interactions with PriB . The function of DnaT is not well understood, but it has been proposed that DnaT binding leads to dissociation of single-stranded DNA (ssDNA) from PriB through a competition mechanism, possibly exposing the ssDNA on the lagging strand template for reloading the replicative helicase, which ultimately leads to fork reactivation .
While studies of DNA replication restart pathways have focused primarily on the well-studied E. coli model organism, DNA replication restart has been shown to be important in other bacteria as well, including the medically important bacterium, Neisseria gonorrhoeae. N. gonorrhoeae is a gram-negative bacterium and the causative agent of gonorrhea. Infections are associated with a host inflammatory response that is mounted against the pathogen involving phagocytic cells such as polymorphonuclear granulocytes . The ability of phagocytes to produce reactive oxygen species as an antimicrobial mechanism has been well-established, and commensal organisms such as lactobacillus species have been shown to produce and secrete H2O2, thus making it likely that N. gonorrhoeae faces considerable oxidative challenges in infected individuals [10, 11].
A variety of studies have examined the sensitivity of N. gonorrhoeae to oxidative stress. Among them, one has demonstrated that N. gonorrhoeae can utilize enzymatic mechanisms such as catalase, peroxidase, and glutathione to protect against reactive oxygen species , another has shown that manganese is important for chemically scavenging superoxide , and yet another has revealed a role for DNA recombination and repair enzymes such as RecA, RecBCD, and enzymes of the RecF-like pathway in resistance to oxidative stress . In addition, PriA has been shown to play a critical role in DNA repair and in resisting the toxic effects of oxidative damaging agents, suggesting that DNA replication restart pathways might play an important role in N. gonorrhoeae resistance to oxidative stress and overall pathogenicity . This notion is further supported by observations that priA from a related bacterium, Neisseria meningitidis, is an important virulence determinant and is important for resisting oxidative injury and promoting bacterial replication .
Studies of DNA replication restart pathways in diverse bacteria such as E. coli and N. gonorrhoeae have revealed species differences in the composition of the DNA replication restart primosome and in the functions of the individual primosome proteins. For example, N. gonorrhoeae lacks a recognizable homolog of dnaT in its genome, suggesting that the N. gonorrhoeae PriA-PriB pathway might be significantly different from the E. coli PriA-PriB-DnaT pathway. Furthermore, physical interactions between primosome components show variation in their individual binary affinities: the physical interaction between PriA and PriB is rather weak in E. coli, but relatively strong in N. gonorrhoeae, and the physical interaction between PriB and ssDNA is strong in E. coli, but relatively weak in N. gonorrhoeae [8, 17, 18]. Thus, the affinities of binary interactions between primosome components are reversed between the two species.
Since the ssDNA-binding activity of PriB is important for PriB-stimulation of PriA's helicase activity in E. coli , there might be significant functional consequences for the variation in affinities of physical interactions within the N. gonorrhoeae PriA-PriB primosome. In this study, we investigated the functional consequences of the affinity reversal phenomenon by examining the helicase activity of N. gonorrhoeae PriA, and we determined how PriA-catalyzed ATP hydrolysis and DNA unwinding are affected by N. gonorrhoeae PriB.
DNA binding by PriA, but not PriB, is structure-specific
We used fluorescence polarization spectroscopy to examine the physical interaction between N. gonorrhoeae PriA and a variety of DNA structures that were constructed by annealing fluorescein-labeled synthetic DNA oligonucleotides. The DNA structures include ssDNA, a partial duplex DNA with a 3' ssDNA overhang, and a forked DNA structure with fully duplex leading and lagging strand arms (Table 1). The presence of a fluorescein tag on the DNAs allowed us to measure PriA binding to the DNA due to the increase in fluorescence polarization of the PriA:DNA complex relative to the unbound DNA. PriA protein was serially diluted and incubated with 1 nM fluorescein-labeled DNA and the fluorescence polarization was measured. Apparent dissociation constants were obtained by determining the concentration of PriA needed to achieve 50% binding to each of the various DNA substrates.
As expected based on studies of E. coli PriA DNA binding [5, 19–22], N. gonorrhoeae PriA binds each of the DNA structures that we tested (Figure 1). PriA binds the forked DNA structure (Fork 2) with the highest affinity of the DNA structures tested, resulting in an apparent dissociation constant of 134 ± 22 nM (Table 2). This DNA structure has fully duplex leading and lagging strand arms with no gap at the three-way junction, and a hydroxyl group exists at the 3' end of the leading strand arm to provide contacts with the 3' hydroxyl binding pocket of PriA's DNA binding domain, assuming that this feature of the helicase has been conserved between the E. coli and N. gonorrhoeae homologs .
The apparent dissociation constants for the partial duplex DNA with a 3' ssDNA overhang and the ssDNA substrate are higher than that of the forked DNA substrate, with values of 234 ± 62 nM (3' Overhang) and 307 ± 43 nM (ssDNA) (Table 2). While we can not rule out the possibility that the differences in affinity are due to differences in the size of the DNA substrates, it is possible that the partial duplex DNA and the ssDNA substrates lack structural elements that are needed to achieve the high affinity binding observed with the forked DNA substrate.
Work from several laboratories has demonstrated that E. coli PriB is a ssDNA-binding protein [18, 24–27], and previous work from our laboratory has shown that N. gonorrhoeae PriB binds ssDNA, albeit with a significantly lower affinity than does the E. coli PriB homolog . Despite this lower affinity, N. gonorrhoeae PriB has the structural hallmark of a ssDNA-binding protein , leading us to hypothesize that it would bind ssDNA and any DNA structures that contain ssDNA with higher affinity than duplex DNAs. To test this hypothesis, we used fluorescence polarization spectroscopy to measure PriB's DNA binding activity using a variety of DNA structures, including ssDNA, double-stranded DNA (dsDNA), a partial duplex DNA with a 3' ssDNA overhang, and a forked DNA structure with fully duplex leading and lagging strand arms (Table 1).
Contrary to expectations, the DNA binding activity of PriB shows little preference for specific DNA structures (Figure 2). The apparent dissociation constants range from 628 ± 95 nM (3' Overhang) to 690 ± 51 nM (Fork 2), and the observed differences among apparent dissociation constants for the various DNA structures are insignificant given the experimental uncertainty of the measurements (Table 2). This observation, together with the low affinity with which N. gonorrhoeae PriB binds DNA relative to E. coli PriB, suggests that the surface of this PriB homolog might have been adapted for a purpose other than binding ssDNA. Furthermore, it raises the important question of whether N. gonorrhoeae PriB can stimulate its cognate PriA's helicase activity, since in E. coli this stimulatory effect depends on PriB's strong ssDNA-binding activity .
PriA helicase activity is limited to short stretches of duplex DNA
To test the functional consequences of N. gonorrhoeae PriB's weak DNA binding activity, we first had to examine N. gonorrhoeae PriA's helicase activity. We used the partial duplex and forked DNA structures shown in Table 1 as substrates based on extensive studies of substrate preference and helicase activity of E. coli PriA [22, 28, 29]. For each of these substrates, the fluorescein-labeled strand represents the nascent lagging strand arm, and the degree of duplex DNA unwinding of the fluorescein-labeled strand was determined using fluorescence polarization spectroscopy. For these experiments, the DNA substrates were incubated with PriA and ATP for 10 min at 37°C, the reactions were terminated by addition of SDS, and the fluorescence polarization of the samples was measured. The degree of unwinding was determined by comparing the fluorescence polarization of the samples to that of the DNA substrate incubated in buffer alone (fully intact DNA substrate) and to the samples heated briefly to 95°C and fast-cooled back to 25°C (fully denatured DNA substrate). This allowed us to measure the fraction of each DNA substrate that is unwound by various concentrations of PriA.
Of the DNA substrates examined, PriA shows greatest unwinding activity on forked DNA substrates with relatively short duplex lagging strand arms. Levels of maximal unwinding are approximately 83% for Fork 1 (15 bp lagging strand arm), 70% for Fork 2 (25 bp lagging strand arm), and 42% for Fork 3 (40 bp lagging strand arm) (Figure 3). PriA shows significantly lower unwinding activity on the partial duplex DNA with a 3' ssDNA overhang. Maximal unwinding activity is approximately 19% for this substrate, suggesting that the partial duplex DNA lacks structural elements required for efficient PriA binding and unwinding (Figure 3). This has been observed for E. coli PriA helicase as well [7, 28]. Overall, these results demonstrate that N. gonorrhoeae PriA helicase activity is limited to relatively short stretches of duplex DNA, akin to its E. coli counterpart.
Comparison of the helicase activity of N. gonorrhoeae PriA that we measured in this study with the previously reported helicase activity of E. coli PriA at the same concentrations and on similar DNA substrates reveals that the two PriA homologs follow the same trend with respect to the dependence of their DNA unwinding activity on the length of the duplex arm of the DNA substrate (Table 3). There are some differences in the degree of DNA unwinding catalyzed by N. gonorrhoeae PriA that we measured in this study compared with the helicase activity previously reported for E. coli PriA. For example, E. coli PriA helicase shows slightly elevated DNA unwinding activity on the 25 bp fork structure compared to N. gonorrhoeae PriA (Table 3). Whether this represents natural biological variation between the two PriA homologs or differences arising from work involving separate investigators is uncertain.
PriB stimulates PriA's helicase activity on long regions of duplex DNA
To determine if N. gonorrhoeae PriB stimulates the helicase activity of its cognate PriA, we examined PriA helicase activity on a forked DNA substrate with a 40 bp lagging strand arm (Fork 3) in the presence and absence of PriB. Under these experimental conditions, incubating 2 nM PriA with Fork 3 in the absence of PriB results in 36% unwinding (Figure 4A). In the presence of PriB, the maximal degree of unwinding is approximately 86%, with near saturating unwinding activity obtained with 20 nM PriB (as monomers). This represents an approximately 2.4 fold stimulation of PriA helicase activity by PriB. Increasing the concentration of PriB to 100 nM (as monomers) does not significantly increase the fold stimulation of PriA helicase activity on this DNA substrate (Figure 4B). E. coli PriB fails to stimulate N. gonorrhoeae PriA helicase activity on Fork 3, indicating that PriB stimulation of PriA helicase activity is species-specific (Figure 4A), and duplex DNA unwinding by PriB is negligible in the absence of PriA, indicating that PriB stimulation of PriA helicase activity is not due to a helicase contaminant in the PriB preparation (Figure 4B).
We also examined PriB's ability to stimulate PriA helicase activity on forked DNA substrates with relatively shorter lagging strand arms. Using 2 nM PriA, we observed a 1.2 fold stimulation of PriA helicase activity on a forked DNA substrate with a 15 bp lagging strand arm (Fork 1), and a 1.7 fold stimulation of PriA helicase activity on a forked DNA substrate with a 25 bp lagging strand arm (Fork 2) (Figure 4B). Therefore, while the overall degree of PriA-catalyzed duplex DNA unwinding decreases as the length of the lagging strand arm increases, the relative stimulatory effect of PriB increases (Tables 3 and 4). This same trend is observed for PriB stimulation of PriA helicase activity in E. coli .
We compared the fold stimulation of N. gonorrhoeae PriA helicase activity by PriB that we measured in this study with that previously reported for E. coli PriA and PriB and found that the fold stimulation is similar for a 40 bp duplex fork structure. In E. coli, PriB stimulates PriA helicase activity 2.6 fold on the 40 bp duplex fork structure, and N. gonorrhoeae PriB stimulates PriA helicase activity 2.4 fold on the same DNA substrate (Table 4). There is a slight difference between the E. coli and N. gonorrhoeae proteins on a 25 bp duplex fork structure. On this DNA substrate, N. gonorrhoeae PriB stimulates PriA helicase activity 1.7 fold, while E. coli PriB does not stimulate PriA helicase activity to a significant degree (Table 4). While the significance of this is unclear, it could be attributed to the relatively lower levels of DNA unwinding by N. gonorrhoeae PriA on this DNA substrate in the absence of PriB compared to that catalyzed by E. coli PriA, thus permitting a greater degree of stimulation of N. gonorrhoeae PriA helicase activity when PriB is present.
We were surprised to observe that N. gonorrhoeae PriB has a stimulatory effect on the DNA unwinding activity of PriA because in E. coli, stimulation of PriA helicase by PriB involves PriB's ssDNA binding activity , which is relatively weak in N. gonorrhoeae PriB . Therefore, we tested the ability of a N. gonorrhoeae PriB variant, PriB:K34A, to stimulate the DNA unwinding activity of its cognate PriA. Amino acid residue K34 of N. gonorrhoeae PriB maps to the ssDNA binding site and is structurally analogous to residue R34 of E. coli PriB, which is involved in binding ssDNA (Figure 5A) . The PriB:K34A variant is defective for ssDNA binding, and a lower limit for the apparent dissociation constant for the interaction of PriB:K34A with ssDNA has been estimated at > 3 μM . The actual dissociation constant could be much higher, but PriB:K34A fails to reach saturable ssDNA binding at the highest protein concentrations that were used in the equilibrium DNA binding assays that were previously reported for this PriB variant .
When we examined PriA helicase activity on Fork 3 in the presence of PriB:K34A, we found that levels of DNA unwinding are similar to those seen when wild type PriB is used to stimulate PriA (Figure 5B). Based on the value of the apparent dissociation constant for the interaction of PriB:K34A with ssDNA, and assuming that it is a reliable indicator of the affinity of PriB:K34A for DNA in the context of a ternary PriA:PriB:DNA complex, we would not expect the PriB:K34A variant to be interacting with DNA to a significant degree under the conditions of this DNA unwinding assay. It is particularly noteworthy that in E. coli, a PriB variant with severely weakened ssDNA binding activity (the W47,K82A double mutant) fails to stimulate the DNA unwinding activity of its cognate PriA to a significant degree . Therefore, unless formation of a PriA:PriB:DNA ternary complex significantly enhances the DNA binding activity of N. gonorrhoeae PriB, our results suggest that ssDNA binding by N. gonorrhoeae PriB does not play a major role in N. gonorrhoeae PriB stimulation of its cognate PriA helicase.
PriB activates PriA's ATPase activity
PriA helicase is thought to couple the energy released from hydrolysis of ATP to the unwinding of duplex DNA. Thus, we wanted to determine if N. gonorrhoeae PriB stimulation of PriA helicase activity involves PriA's ability to hydrolyze ATP. To examine PriA's ATPase activity, we used a spectrophotometric assay that couples PriA-catalyzed ATP hydrolysis to oxidation of NADH. This assay allowed us to measure steady-state PriA-catalyzed ATP hydrolysis rates in the presence and absence of PriB. As expected, PriA's ATPase activity is negligible in the absence of DNA (Figure 6A). The DNA dependence of PriA's ATPase activity has been observed in E. coli as well , and likely reflects a mechanistic coupling of ATP hydrolysis and duplex DNA unwinding.
With 10 nM PriA and in the absence of PriB, near maximal rates of ATP hydrolysis are observed with 10 nM Fork 3 (Figure 6A). Under these experimental conditions, the Km with respect to DNA is 2 ± 1 nM and the kcat is 9 ± 1 s-1 (Table 5). To determine if PriB affects the ATPase activity of PriA, we measured ATP hydrolysis catalyzed by 10 nM PriA in the presence of 100 nM PriB (as monomers) and various concentrations of Fork 3 DNA (Figure 6A). This produces the same ratio of PriB to PriA that results in near maximal stimulation of PriA helicase activity (Figure 4A). Addition of 100 nM PriB (as monomers) yields a Km with respect to DNA of 3 ± 1 nM (Table 5). Thus, the presence of PriB has no significant effect on PriA's Km with respect to DNA. We also examined the effect of PriB on PriA's Km with respect to ATP (Figure 6B). With 10 nM PriA and in the absence of PriB, the Km with respect to ATP is 54 ± 19 μM (Table 5). Addition of 100 nM PriB (as monomers) yields a Km with respect to ATP of 70 ± 13 μM (Table 5). Thus, the presence of PriB has no significant effect on PriA's Km with respect to ATP.
While PriB does not have a significant effect on PriA's Km values for ATP or DNA, it does have a significant effect on the value of kcat. In the presence of 100 nM PriB (as monomers), the kcat increases to 14 ± 1 s-1, indicating that PriB activates PriA's ATPase activity (Figure 6 and Table 5). This observation lies in contrast to studies performed using E. coli PriA and PriB proteins that reveal no effect of PriB on the rate of PriA-catalyzed ATP hydrolysis .
In this study, we examined physical interactions within the DNA replication restart primosome of N. gonorrhoeae and the functional consequences of those interactions to gain insight into the biological significance of species variation in primosome protein function. Physical interactions within the DNA replication restart primosome of N. gonorrhoeae differ in several ways compared to those within the DNA replication restart primosome of E. coli. In E. coli, the PriA:PriB binary interaction is weak, while the PriB:DNA binary interaction is strong. In N. gonorrhoeae, these affinities have been reversed: the PriA:PriB binary interaction is strong, while the PriB:DNA binary interaction is weak.
The crystal structure of N. gonorrhoeae PriB provides clues that could account for the low affinity PriB:DNA interaction. Analysis of the binding site for DNA reveals significantly reduced positive electrostatic surface charge potential relative to the analogous surface of E. coli PriB, and several aromatic residues of E. coli PriB that are known to play a role in binding ssDNA are not conserved in N. gonorrhoeae PriB [17, 18]. Furthermore, our results indicate that N. gonorrhoeae PriB shows little preference for binding specific DNA structures. Thus, it is possible that some portion of the surface of PriB involved in DNA binding in E. coli has been adapted for another purpose in N. gonorrhoeae, perhaps for interactions with its cognate PriA. This could explain the high affinity PriA:PriB interaction seen in N. gonorrhoeae relative to E. coli.
Despite variation in the affinities of individual binary interactions within the two bacterial primosomes, we have found that the functional consequences of the physical interactions appear to be similar between the two species in one important way: formation of a PriA:PriB:DNA complex stimulates the helicase activity of PriA. More interesting, however, are the mechanistic details of how this stimulation is accomplished. In E. coli, evidence suggests that a ssDNA product-binding mechanism is important for PriB stimulation of PriA helicase activity, likely within the context of a PriA:PriB:DNA ternary complex . Furthermore, PriB has no effect on the rate of PriA-catalyzed ATP hydrolysis in E. coli . This indicates that allosteric activation of PriA's ATPase activity is not a key factor in the stimulation of PriA helicase by PriB in E. coli.
While we can not rule out a ssDNA product-binding mechanism operating in N. gonorrhoeae DNA replication restart, the relatively low affinity with which N. gonorrhoeae PriB binds ssDNA suggests that this type of mechanism might not contribute as much to PriB stimulation of PriA helicase activity in N. gonorrhoeae as it does in E. coli. This hypothesis is further supported by the observation that a N. gonorrhoeae PriB variant with greatly diminished ssDNA binding activity can stimulate the helicase activity of PriA at nearly the same levels as does wild type PriB. On the other hand, an allosteric activation mechanism could account for PriB stimulation of PriA helicase in N. gonorrhoeae. This form of activation would not necessarily require a high affinity PriB:DNA interaction, and could arise from a conformational change induced in PriA upon binding PriB, thus enhancing the rate at which PriA hydrolyzes ATP and couples ATP hydrolysis to the process of unwinding duplex DNA. An allosteric activation model could also provide a potential functional consequence for the high affinity PriA:PriB interaction observed in N. gonorrhoeae.
Despite differences in binary affinities among primosome components, the function of the primosome proteins in these two bacterial species appears to converge on a similar outcome: stimulation of PriA helicase by its cognate PriB. This raises the question of why such differences would have been selected for throughout evolution. One possible explanation lies with the presence of DnaT in E. coli and its apparent absence in N. gonorrhoeae. In E. coli, DnaT is believed to play an important role in primosome assembly and might facilitate the release of ssDNA from PriB within the primosome complex, perhaps making the ssDNA available for binding by the replicative helicase [8, 31]. Without the stabilizing weak interactions provided by DnaT, a primosome complex might require an alternate source of weak interactions to achieve an equivalent level of stability. In N. gonorrhoeae, a robust PriA:PriB interaction might supply the requisite primosome-stabilizing binding energy that would have otherwise come from DnaT in an organism such as E. coli.
Furthermore, the lack of DnaT in N. gonorrhoeae could explain the relatively weak affinity with which its PriB binds ssDNA. With no DnaT to facilitate release of ssDNA from PriB, as is thought to occur in E. coli, N. gonorrhoeae might require its PriB to have an inherently low affinity for ssDNA to promote release of ssDNA without assistance, assuming that PriB actually binds ssDNA in N. gonorrhoeae cells. It is possible that some portion of the DNA binding site of N. gonorrhoeae PriB has been remodeled to accommodate interactions with its cognate PriA, thereby sacrificing interactions with DNA for enhanced interactions with PriA that could activate PriA's ATPase activity.
Another possible explanation for the differences seen between the two species is that physical interactions among components of the N. gonorrhoeae DNA replication restart primosome could have become specialized to meet the physiological demand for DNA replication restart in N. gonorrhoeae cells, which likely differs from that in E. coli cells. A high affinity interaction between PriA and PriB might indicate that PriA and PriB are constitutively complexed with one another in N. gonorrhoeae cells, perhaps facilitating a more rapid response to DNA damage than could be elicited by primosome proteins that must assemble at a site of DNA replication fork reactivation. This type of adaptation could be particularly beneficial for an organism such as N. gonorrhoeae that has evolved under selective pressure to withstand relatively high levels of oxidative damage to its genome.
The results of this study demonstrate that a bacterial PriB homolog with weak single-stranded DNA binding activity can stimulate the DNA unwinding activity of its cognate PriA helicase. While it remains possible that N. gonorrhoeae PriB binds DNA in the context of a PriA:PriB:DNA ternary complex, in which the local concentration of DNA could be quite high, our results suggest that N. gonorrhoeae PriB might have evolved to interact strongly with PriA instead of with DNA, thus sacrificing high affinity DNA binding for protein:protein interactions with PriA that could modulate PriA's helicase activity. This could account for N. gonorrhoeae PriB's ability to stimulate PriA-catalyzed ATP hydrolysis, which is a function not observed with E. coli PriA and PriB proteins.
DNAs and proteins
The priA and priB genes of N. gonorrhoeae were cloned and the recombinant PriA and PriB proteins were purified as previously described . DNA substrates used in the equilibrium DNA binding assays and duplex DNA unwinding assays were constructed by annealing complementary DNA oligonucleotides, one of which in each structure was labeled with fluorescein at the 3' end (Table 1). Oligonucleotides were suspended in 10 mM Tris·HCl pH 8, 50 mM NaCl, 1 mM EDTA at a 2:1 molar ratio of non-labeled DNA to fluorescein-labeled DNA. The DNAs were incubated at 95°C for 5 min, slow-cooled to 70°C and incubated at that temperature for 60 min, and slow-cooled to 25°C. Duplex DNAs were gel-purified through 6% polyacrylamide gels using 100 mM Tris borate pH 8.3, 2 mM EDTA as the electrophoresis buffer. The DNAs were excised from the polyacrylamide gels, electroeluted using the same electrophoresis buffer, dialyzed against 10 mM Tris·HCl pH 8, 5 mM MgCl2, aliquoted, and stored at -20°C.
Equilibrium DNA binding assays
Fluorescence polarization spectroscopy was performed at 25°C with a Beacon 2000 fluorescence polarization system (Invitrogen). Serial dilutions of PriA or PriB were made into 20 mM Tris·HCl pH 8, 10% (v/v) glycerol, 50 mM NaCl, 1 mM 2-mercaptoethanol, 0.1 mg/ml bovine serum albumin (BSA) and incubated with 1 nM fluorescein-labeled DNA. Apparent dissociation constants (Kd,app) were calculated by determining the concentration of either PriA or PriB required to bind 50% of the fluorescein-labeled DNA (Curve Expert 1.3). The unbound state is reported by the fluorescence anisotropy of the fluorescein-labeled DNA in the presence of buffer alone. The fully-bound state is reported by the fluorescence anisotropy of the fluorescein-labeled DNA in the presence of a sufficient concentration of PriA or PriB to saturate the fluorescence anisotropy signal. Data are reported in triplicate and associated uncertainties represent one standard deviation of the mean.
DNA unwinding assays
DNA substrates were diluted to 1 nM in 20 mM Tris·HCl pH 8, 50 mM NaCl, 3 mM MgCl2, 1 mM 2-mercaptoethanol, 1 mM ATP. For unwinding assays involving PriB proteins, indicated concentrations of wild type PriB or PriB:K34A were added to the DNA and incubated for 5 min on ice. Indicated concentrations of PriA were added to the reaction mixtures and incubated at 37°C for 10 min to facilitate duplex DNA unwinding. Reactions were stopped by addition of SDS to a final concentration of 1%. The amount of duplex DNA unwound was determined by measuring the fluorescence anisotropy of the samples following addition of SDS. Fluorescence anisotropy values were compared to the fluorescence anisotropy of the DNA substrate incubated in buffer alone (fully intact DNA substrate) and the fluorescence anisotropy of the DNA substrate after being heated to 95°C and rapidly cooled to 25°C (fully denatured DNA substrate) to calculate the fraction of DNA unwound. Data are reported in triplicate and associated uncertainties represent one standard deviation of the mean.
ATP hydrolysis assays
PriA-catalyzed ATP hydrolysis was measured using a coupled spectrophotometric assay that has been previously described . This assay uses an ATP regeneration system that converts ADP to ATP in a reaction that is coupled to the conversion of NADH to NAD+. The coupled reaction can be monitored spectrophotometrically by measuring the decrease in absorbance at 340 nm due to NADH oxidation. Primosome proteins at indicated concentrations were incubated with indicated concentrations of DNA and ATP in 20 mM Hepes pH 8, 50 mM NaCl, 7 mM 2-mercaptoethanol, 2 mM phosphoenol pyruvate, 0.1 mM NADH, 14 units/ml pyruvate kinase, 20 units/ml lactate dehydrogenase, 0.1 mg/ml BSA at 25°C. Steady-state Δ[NADH]/Δt rates were calculated using the molar extinction coefficient 6,220 M-1·cm-1 for NADH, and these rates are equivalent to Δ[ATP]/Δt. The kinetic parameters Km and kcat were determined with respect to DNA and with respect to ATP by fitting the ATP hydrolysis rates to the Michaelis-Menten equation,
where S = either DNA or ATP (Curve Expert 1.3). Values of kcat were determined by dividing Vmax by the concentration of PriA in the reaction. Data are reported in triplicate and associated uncertainties represent one standard deviation of the mean.
- The abbreviations used are:
ssDNA: single-stranded DNA
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This work was supported by grants from Research Corporation for Science Advancement and from the University of Dayton Research Council to MEL, and by grants from the University of Dayton Graduate School to CF and BS.
CF, BS, and MEG purified the proteins, constructed the DNA substrates, and carried out the equilibrium DNA binding assays, DNA unwinding assays, and ATP hydrolysis assays. MEL conceived of the study, participated in its design and execution, and drafted the manuscript. All authors read and approved the final manuscript.
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Feng, C., Sunchu, B., Greenwood, M.E. et al. A bacterial PriB with weak single-stranded DNA binding activity can stimulate the DNA unwinding activity of its cognate PriA helicase. BMC Microbiol 11, 189 (2011). https://doi.org/10.1186/1471-2180-11-189
- Fluorescence Anisotropy
- Helicase Activity
- Apparent Dissociation Constant
- ssDNA Binding
- Fold Stimulation