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Table 2 Oligonucleotides used in the present study

From: Campylobacter jejuni dsb gene expression is regulated by iron in a Fur-dependent manner and by a translational coupling mechanism

Name

Sequence

Orientation//restriction site

H0B

GTCTAGGATCC GCTTGATATCGCTGATTACT

Fwd//BamHI

H4X

ATCTGTCTAGAGCCAGCAGGAGCAATTACATCT

Rev//XbaI

Cj16RS

GCAGTCGAC TCAATGAAGGTAAGAGTAAG

Rev//SalI

Cj17Bgl

CCTAGATCT AGCCTGCTAAACACATTAGT

Rev//BglII

Cj17Nde

GTACAT ATGAACGAAATCAATAAAAC

Fwd//NdeI

Cj17RBgl

TTCAGATCT CTAATGTGTTTAGCAGGC

Fwd//BglII

Cj17RM

TATGAATTC AGGAATACCTGTGCTAACAA

Rev//EcoRI

Cj17LSal

GCTGTCGAC TGATAAGAAAGAATATTG

Rev//SalI

Cj17WDBam-low

GGATCC TGTGGGGAGTGCGATAG

Rev//BamHI

Cj17WDBam-up

GGATCC ACAGGTATTCCTCCTTATGTAG

Fwd//BamHI

Cj18Bgl

CCTAGATCT GATAATCAGTATCAAGGCGA

Rev//BglII

Cj18L29

CCAAGCTACCATTACCtAACCAAAAGCCAAAT

Rev//Ø

Cj18L29_c

ATTTGGCTTTTGGTTaGGTAATGGTAGCTTGG

Fwd//Ø

Cj18LM

TATGGATCC CAGGAGCACTATTAACAATA

Fwd//BamHI

Cj18M1R

AAAGTTCAAGAAACTCCcTAGTATCTCCTTTG

Rev//Ø

Cj18M1Rc

CAAAGGAGATACTAgGGAGTTTCTTGAACTTT

Fwd//Ø

Cj18Nde-Rev

ATA CTGCAG CAAGAAACTC CATATG ATCTCC

Rev//PstI, NdeI

Cj18RM

TGAGGATCCAAGCCAAGCTACCATTACCA

Rev//BamHI

Cj19LX-2

AGTTCTAGA AGTTGGACAGCTTGCTGATA

Fwd//XbaI

Cjj43Eco

GCAGAATTC AAGCATAGCAGGATCTTTGG

Rev//EcoRI

Cjj43mwL

CGTGGATCCCCGGGTA GGACTTATATTTAATC

Fwd//SmaI, BamHI

Cjj44X

AGTTCTAGA CATTTAGCCCTTCCTTACAG

Rev//XbaI

Cjj45B

ATCGGATCC GATACTATGGAGTTTCTTGA

Fwd//BamHI

Cjj45Dig

TCAAGAAACTCCATAGTATC

Rev//Ø

Cjj46

CATGTGAAATCAATAATATC

Fwd//Ø

Cjj46mwR

ATACCCGGGGATCCA AAGTTACTGAAAGCTAC

Rev//SmaI, BamHI

Cj-RT

GCAGTCGAC TAGGATCGATAGTAGCTGAA

Rev//SalI

Cjj879B

ATCGGATCC CACAATCTAAAGGGTATTTC

Fwd//BamHI

Cjj880

CTTATCCATAAAATATAATG

Fwd//Ø

Cjj880B

ATCGGATCC ACCTAGATTATTCTACTTTG

Fwd//BamHI

Cjj880B2

ATCGGATCC TTTCCAGTAAAATTAGCAAG

Fwd//BamHI

Cjj880X

AGTTCTAGA CACATACAACAATAGATCTT

Rev//XbaI

Cjj881B

ATCGGATCC AAAATGAAAGATAATTGCAG

Fwd//BamHI

Cjj881X

AGTTCTAGA AAATGTGCTATACAAGTAAG

Rev//XbaI

Cjj882

AGGTGTAAGTCTTGGAGAGC

Fwd//Ø

Cjj882B

ATGGATCC GACTTAGCAAAACTCTTTGT

Fwd//BamHI

Cjj882X

AGTTCTAGA AGAGTTTTGCTAAGTCTCAT

Rev//XbaI

Cjj882B3

ATCGGATCC ATGATGATTATAGCAAATGC

Fwd//BamHI

Cjj883X2

AGTTCTAGA GCTAATGCAAAACTTGAATA

Rev//XbaI

CM-L

ATATCACGCAATTAACTTGG

Rev//Ø

CM-R

GGATGAATTACAAGACTTGC

Fwd//Ø

DIG_dsbA1

GCTAATGCAAAACTTGAATA

Rev//Ø

DIG_dsbA2X

CACATACAACAATAGATCTTG

Rev//Ø

DIG_chuF

CATATGAGAAATAATGCTTTC

Fwd//Ø

EMSAchuR

TTTGGGTGCAAATTTTACTC

Rev//Ø

Fur-L

TGAATTTTTATTGGTTTGATGC

Fwd//Ø

Fur-R

TCCTCATCTTCAATGTTTGC

Rev//Ø

KAN-L

TATCACCTCAAATGGTTCGCTGGG

Rev//Ø

KAN-R

GGGGATCAAGCCTGATTGGGAGA

Fwd//Ø

KM-L1

GAGAATATCACCGGAATTGA

Fwd//Ø

KM-R1

CTTCATACTCTTCCGAGCAA

Rev//Ø

lacZ

AGGTTACGTTGGTGTAGATG

Rev//Ø

lacZ1

GGAATTCACTGGCCGTCGTT

Fwd//Ø

  1. Bold letters indicate C. jejuni 81-176 sequences; restriction recognition sites introduced for cloning purposes are underlined, complementary fragments of primers Cjj46mwR and Cjj43mwL are marked with italics. Point mutated nucleotides in primers are marked with small letters. Orientation of the primers (Fwd states for forward/Rev - for reverse) refers to the orientation of particular C. jejuni gene studied. RT-Cj primer was designed on the basis of C. coli 72Dz/92 dsbI nucleotide sequence (there are 2 nucleotide changes compared to the nucleotide sequence of its orthologue from C. jejuni 81-176).
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BMC Microbiology

ISSN: 1471-2180

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