Stationary-phase survival under high salinity. Cells were grown in LB containing either 15% (A, B) or 25% (C, D) NaCl. A, C: ODs were measured at least twice, and the means are shown. B, D: The number of CFUs was determined at least three times. The means and standard deviations are shown. E: Thin-layer chromatography of phospholipids. Note that CL accumulated in the cls2 mutant. The relative signal intensities are shown on the right.