Figure 1

Dynamic range and sensitivity of the Campylobacter coli and Campylobacter jejuni real-time PCR assays. Standard curves of 10-fold serial dilution of standard DNA of (a) C. coli CIP 70.81 (from 0.3 × 10¹ to 3.0 × 10⁸ genome copies per PCR reaction) and of (b) C. jejuni NCTC 11168 (from 10¹ to 10⁸ genome copies per PCR reaction) are reported, each dot representing the result of duplicate amplification of each dilution. The coefficients of determination R² and the slopes of each regression curve are indicated. The standard curves are obtained by correlation of the threshold cycle values (Ct) and log₁₀ input genome copy number (Log CO) from the amplification plot.