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Table 3 Phosphatases in cell extracts of impA, suhB mutants

From: Inositol monophosphate phosphatase genes of Mycobacterium tuberculosis

Substrate

H37Rv

ΔimpA

ΔsuhB

Fructose-1,6-bisP

26.04 ± 1.85 (5)

28.18 ± 0.92 (5)

32.70 ± 0.44 (5)

Inositol-1-P

0.63 ± 0.13 (6)

0.79 ± 0.12 (5)

0.63 ± 0.25 (6)

Inositol-2-P

1.20 ± 0.15 (4)

1.33 ± 0.22 (5)

1.03 ± 0.15 (6)

Glycerol-2-P

0.08 ± 0.06 (12)

-0.02 ± 0.03 (2)

0.39 ± 0.03 (2)

Glycerol-3-P

-0.13 ± 0.12 (12)

-0.08 ± 0.03 (2)

0 ± 0.21 (2)

5' AMP

4.22 ± 0.36 (8)

4.13 ± 0.40 (2)

5.74 ± 0.04 (2)

p-nitrophenyl-P

3.00 ± 0.35 (12)

3.55 ± 0.14 (2)

4.38 ± 0.36 (2)

  1. Values: nmol/min/mg protein, mean ± SEM (n). Differences between levels in mutants and the parent strain were not significant (P > 0.05; t-test).