Figure 7
Catabolite repression control in expression of the benA , catB or pcaD genes in the presence of 4 mM benzoate. Cells were harvested and transferred into minimal medium supplemented with succinate, lactate, acetate or glucose. To induce the catabolic promoter, benzoate was added to logarithmically growing cultures. Cultures were incubated at 30°C for 2 h, and samples were collected for quantitative real-time RT-PCR analysis.