Table 1 Primers used in this study
From: Proteomic analysis of Salmonella enterica serovar Enteritidis following propionate adaptation
Primer name | Sequence (5'→3') | Amplification target |
|---|---|---|
CpxR-F Long | CCAGCACTTCCTGGCTTAAATGTTCACGGG | cpxR in qRT-PCR |
CpxR-R Long | GGCCAAACGCTGGAGCTGACCGGTACGG | cpxR in qRT-PCR |
SodA-F Long | GGTCACGATCCACGTTATGCCAAACTGAGCG | sodA in qRT-PCR |
SodA-R Long | GATCGGGAAGCCGGAAGCGCCGGAAATGG | sodA in qRT-PCR |
rplF-F Long | GGTCAACCGGGTGAGAGAAACCTAAAGACAGG | rplF in qRT-PCR |
rplF-R Long | GGCTTCACTAAGAAGCTGCAGCTGGTTGG | rplF in qRT-PCR |
rplE-F Long | GGGAAGATGATCTGCTCACGGACACCCATGC | rplE in qRT-PCR |
rplE-R Long | GCCTGATCACTATTGCTGTTCCTCGTATCCG | rplE in qRT-PCR |
dps-F Long | CGGCCAGTTCTTTTAAGTGATCCTGCACG | dps in qRT-PCR |
dps-R Long | GGCGTTAGGCACCACGCAAGTTATCAACAGC | dps in qRT-PCR |
16 s rRNA-FP | GGTAGCCAACTGCTGCTGTCT | 16 S rRNA in qRT-PCR |
16 s rRNA-RP | CCGCAGCCGACATCCA | 16 S rRNA in qRT-PCR |
CpxR-P1 | GTTGATGATGACCGAGAGCTGACTTCCCTGTGTGTA GGCTGGAGCTGCTT | KmR gene from pKD4; specific for deletion of cpxR |
CpxR-P2 | AGCGGAAACCATCAGATAGCCGCGACCACGCATATG AATATCCTCCTTAG | KmR gene from pKD4; specific for deletion of cpxR |
CpxR-UpF | CTGCTGACGCTGATGTTCGG | region upstream of cpxR |
CpXR-UpR | CAGGGAAGTCAGCTCTCGGTC | region upstream of cpxR |
CpxR-DwnF | CGTGGTCGCGGCTATCTGATGG | region downstream of cpxR |
CpxR-DwnR | GCGGATGATCGGCGTTATCCGC | region downstream of cpxR |
dps-P1 | TGCTTTATACCCGTAACGATGTATCAGAGAGCGTGT GTAGGCTGGAGCTGCTT | KmR gene from pKD4; specific for deletion of dps |
dps-P2 | AGGTCGCGTGATGCGGCGGTAAAGATATCGGCCATA TGAATATCCTCCTTAG | KmR gene from pKD4; specific for deletion of dps |
dps-UpF | CTGACCAGCATCGTGACAATGAGC | region upstream of dps |
dps-UpR | CGCTCTCTGATACATCGTTACGG | region upstream of dps |
dps-DwnF | GCCGATATCTTTACCGCCGC | region downstream of dps |
dps-DwnR | GGGCAAAACCAGTATGCCGCACC | region downstream of dps |