Transcellular transport of West Nile virus-like particles across human endothelial cells depends on residues 156 and 159 of envelope protein
© Hasebe et al; licensee BioMed Central Ltd. 2010
Received: 13 January 2010
Accepted: 8 June 2010
Published: 8 June 2010
West Nile virus (WNV) causes viremia after invasion to the hosts by mosquito bite. Endothelial cells could play an important role in WNV spread from the blood stream into the central nervous system and peripheral tissues. Here, we analyzed the capacity of virus-like particles (VLPs) of the highly virulent NY99 6-LP strain (6-LP VLPs) and the low virulence Eg101 strain (Eg VLPs) to cross cultured human endothelial cells.
6-LP VLPs were transported from the apical to basolateral side of endothelial cells, whereas Eg VLPs were hardly transported. The localization of tight junction marker ZO-1 and the integrity of tight junctions were not impaired during the transport of 6-LP VLPs. The transport of 6-LP VLPs was inhibited by treatment with filipin, which prevents the formation of cholesterol-dependent membrane rafts, suggesting the involvement of raft-associated membrane transport. To determine the amino acid residues responsible for the transport of VLPs, we produced mutant VLPs, in which residues of E protein were exchanged between the 6-LP and Eg strains. Double amino acid substitution of the residues 156 and 159 greatly impaired the transport of VLPs.
Our results suggest that a transcellular pathway is associated with 6-LP VLPs transport. We also showed that the combination of the residues 156 and 159 plays an important role in the transport of VLPs across endothelial cells.
West Nile virus (WNV), a mosquito-borne single-stranded RNA virus, had been known to cause endemic febrile disease in Africa, the Middle East, Europe and Asia [1–4]. Since the concurrent outbreaks of encephalitis among humans, horses and birds in New York in 1999 [5–7], WNV has spread rapidly across North America . WNV has considerable public health impact because of large annual epidemics of human neuroinvasive disease . WNV proliferates in birds and is transmitted to humans, horses and other animals by mosquitoes. After invading the hosts, WNV seems to proliferate in lymphoid tissue and causes viremia . WNV then penetrates the blood brain barrier (BBB) and causes encephalitis with neuronal cell death. Neurons are the main target of the virus in the central nervous system (CNS), since viral antigens are mainly detected in these cells .
In addition to the neuronal disease, WNV-associated inflammation outside the CNS can occur in humans. Khouzam  reported the case of a patient who had diffuse myocardial damage secondary to WNV infection. Rhabdomyolysis was reported in a patient with WNV encephalitis . Armah et al.  reported systemic distribution of WNV infection in 6 human cases in which viral antigens were detected in CNS, kidney, lungs, pancreas, thyroid, intestine, stomach, esophagus, bile duct, skin, prostate and testis. These studies suggest that WNV can invade and proliferate in multiple tissues.
Shirato et al.  suggested that the difference in the neuroinvasiveness between the highly virulent NY99 strain and the non-lethal Eg 101 (Eg) strain is associated with the viral replication in spleen. One of the reasons NY99 strain gains this virulent phenotype might be an enhancement of invasiveness to the peripheral tissues. Blood-borne pathogens must encounter endothelial cells of blood capillaries to invade the target organs. Verma et al.  demonstrated the mechanism by which WNV crosses endothelial cells using human brain microvascular endothelial (HBMVE) cell culture. Their data suggested that WNV crosses HBMVE cells via a transcellular pathway after viral replication in endothelial cells. However, the possibility that WNV crosses endothelial cells without viral replication cannot be excluded, since WNV infection of endothelial cells is rarely detected in human cases . It is still unclear if a transcellular mechanism is also involved in viral invasion to endothelial cells of peripheral tissues.
In this study, we assessed the possibility that WNV has an ability to cross human endothelial cells. To eliminate the influence of viral replication in endothelial cells, we used virus-like particles (VLPs) which can infect susceptible cells without production of progeny virions. Our results suggest that VLPs of the NY99-6922 6-LP (6-LP) strain cross human umbilical vein endothelial cells (HUVEC) by a transcellular pathway. We also showed that the 6-LP VLPs were transported greater than VLPs of the low-virulence Eg strain, which depends on Ser 156 and Val 159 of E protein.
WNV 6-LP VLPs are transferred across human endothelial cells
HUVEC were seeded on the membranes of transwells, which have 0.4 μm pores. The presence of the tight junction with an increase of transendothelial electrical resistance (TEER; 66-77 Ωcm2) was confirmed 3 days after seeding. Here we used VLPs previously reported by Scholle et al. . VLPs can infect cells because of the presence of the structural proteins (C, prM/M and E protein) that are present in infectious virions. VLPs contain replicon RNA, which encodes the WNV nonstructural proteins and the enhanced green fluorescent protein (eGFP), but lacks the sequence of structural proteins. After VLP infection of susceptible cells, replicon RNA is released and replicates in the cytoplasm accompanied by the expression of eGFP. However, progeny particles are not produced because of the lack of expression of structural proteins in VLP-infected cells.
6-LP VLPs were transported without altering the integrity of tight junction
Verma et al.  suggested that WNV replicates in the HBMVE cells and that the progeny virus crosses the BBB via a transcellular pathway without impairing the integrity of tight junction. However, VLPs used in this study do not produce progeny virions. Thus, there is a possibility that 6-LP VLPs cross from the apical to the basolateral side by disrupting tight junction.
6-LP VLPs cross HUVEC via a transcellular pathway
To assess the involvement of a transcellular pathway, we examined the effects of chlorpromazine and filipin on VLP transport. Chlorpromazine disrupts the recycling of AP-2 from endosomes and prevents the assembly of clathrin-coated pits on the plasma membrane . Filipin is a sterol-binding agent and prevents the formation of cholesterol-dependent membrane rafts . The optimal concentration of chlorpromazine and filipin was determined by the inhibition of the uptake of transferrin and cholera toxin subunit B, which are known as ligands for clathrin-and lipid-rafts-dependent endocytosis, respectively (data not shown). HUVEC were exposed to 6-LP VLPs in the presence or absence of the inhibitor. FITC-labeled 70k Dx was also added to the transwells with 6-LP VLPs to evaluate the tight junction integrity. The transport of VLPs was inhibited by filipin (p < 0.01), but was not significantly by chlorpromazine (Fig. 2C). In contrast, the permeability of 70k Dx was not impaired by either chlorpromazine or filipin (Fig. 2D). These results suggested that lipid rafts are involved in VLPs transport.
Transport of 6-LP VLPs depends on E protein
Multiple amino acid residues of E protein influence the transport of 6-LP VLPs
Single and double mutant VLPs
6-LP S156P V159I
Eg P156 S I159V
The combination of Ser 156 and Val 159 is important for the transport of 6-LP VLPs
Combination of amino acid sequence at 156 and 159 does not affect the N-linked glycosylation of E protein
WNV NY strains have a highly virulent phenotype compared to the Eg strain which was isolated in Africa. Their enhanced replication in peripheral tissues may lead to long-lasting viremia resulting in increasing incidence of viral invasion to CNS. The interaction of the virus with endothelial cells of blood capillaries could be involved in WNV invasion to target organs. In this study, we assessed the transport of WNV NY99 6-LP strain and Eg strain across human endothelial cells. Our data demonstrate that VLPs of the 6-LP strain were transported across human endothelial cells more than VLPs of the Eg strain.
Microbial invasion across endothelial cells can occur through transcellular pathway mediated by vesicles, paracellular entry after disruption of the tight junctions, or "Trojan horse" mechanism by transport within circulating phagocytic cells [35, 36]. Our data indicate that 6-LP VLPs are transported by a transcellular pathway, because the transport of VLPs was inhibited by the treatment with filipin, a modifier of lipid raft-associated membrane transport. Clathrin-dependent pathways seem to be less important because the treatment with chlorpromazine had no significant effect on the transport of VLPs. Paracellular entry is unlikely to be involved in transport of VLPs because the structure of ZO-1 and the permeability of Dx 70k were not altered during VLP transport.
Our data partially support the results by Verma et al.  which suggested that WNV crosses HBMVE cells without altering the integrity of tight junction. The authors concluded that WNV replicates in endothelial cells and the progeny viruses are transported from the apical to basolateral side. However, our data suggest that WNV can be transported across endothelial cells without viral replication. Cell type difference could be the most reasonable explanation, because several studies showed that there are differences between HBMVE cells and HUVEC in the production of growth factors, immunoregulatory factors and adhesion molecules [37–39]. HBMVE cells and HUVEC differentially respond to cytokine treatment resulting in the different cytokine production and leukocyte recruitment [40, 41]. Particularly, modulation of adhesion molecules can affect endocytosis . Therefore, our data seem to reflect events that can occur in peripheral tissues having tight junction such as heart and muscles rather than in CNS.
In WNV-infected mice, viral replication in peripheral tissues results in the inflammatory cytokine production such as TNF-α, IL-6 and macrophage migration inhibitory factor [42–45]. Although the role of these cytokines in infection still remains controversial, vascular permeability can be affected by the presence of these cytokines . One of the mechanisms for the impairment of vascular permeability by these cytokines is disruption of tight junctions of endothelial cells . Promotion of vesicular transport of endothelial cells, including pinocytosis and transcytosis, is also affected by these cytokines . Paracellular invasion by disruption of the tight junction induced by cytokines could occur in vivo, however, there is a possibility that WNV also utilizes a transcellular pathway, which might be promoted by inflammatory cytokines.
The analysis of VLPs with chimeric E proteins showed that E protein determines the difference in the transport across HUVEC between the 6-LP and Eg strains. Our data also suggest that multiple amino acid residues of E protein are influential. It has been well known that the sequence NYS/T at the residues 154-156 is important for glycosylation associated with the virulence of WNV and that strains possessing proline at the residue 156 lack glycosylation [10, 31–33]. The prototype WNV strain B956 has a 4 amino acid deletion in the residues 156-159 resulting in absence of glycosylation . The position of glycosylation seems to be also important, since the WNI-25 and WNI-25A strains which have N-glycosylation at the residue 155, do not show neuroinvasive phenotype [49, 50]. The present study suggests that the combination of Ser 156 and Val 159 is important for transport of VLPs across endothelial cells, which might be associated with the invasion of WNV into the target organs.
The transport of Eg P156 S VLPs was lower than that of WT Eg VLPs in spite of the presence of glycosylation. The residues 156-160 form two turns of α-helix, termed αA', although E proteins of Dengue virus serotype 2 (DENV-2) and Tick-borne encephalitis virus (TBEV) lack the amino acids 157-160 resulting in the absence of this structure. The α-helix shifts the glycosylation site about 5 Å to the exterior and lateral surfaces of E protein with respect to those of E proteins of DENV-2 and TBEV. Davis et al.  showed that modulation of N-glycosylation of WNV E protein modified the attachment to DC-SIGNR. As well as the existence of proline and the deletion of the amino acids between the residues 156-160, there is a possibility that the combination of amino acid residues at 156 and 159 might affect the structure of αA' and position of glycosylation site, resulting in modulation of the binding affinity to a lectin or unknown binding molecules on HUVEC. This, in turn, could be a reason for the unsuccessful transport of Eg P156 S VLPs.
In this study, we propose a transcellular mechanism by which WNV crosses endothelial cells and enters the target organs. We also suggest that higher transendothelial migration ability could be one of the determinants of the different virulence of the NY and Eg strains, and that this depends on Ser 156 and Val 159 of E protein.
HUVEC were purchased from Lonza Group Ltd. and cultured in EGM-2 Endothelial Cell Growth Medium-2 supplemented with SingleQuots growth factors, cytokines and supplements (Lonza). The cells at passage 5 were used for experiments. Vero cells were cultured in Eagle's minimum essential medium (MEM; Nissui, Tokyo, Japan) supplemented with 5% fetal bovine serum (FBS; Sigma). Baby hamster kidney (BHK) cells were cultured in MEM supplemented with 10% FBS. HEK293T cells were cultured in Dulbecco's Modified Eagle Medium (Nissui).
The WNV 6-LP and Eg strains were provided by Dr. I. Takashima, Hokkaido University, Japan [15, 34]. 6-LP strain was established by plaque purification from WNV NY99-6922 strain, which was isolated from mosquitoes in 1999 . Complement DNA (cDNA) of the structural genes (C, prM/M and E) of the 6-LP and Eg strains were prepared by RT-PCR and subcloned into pCXSN, which was generated from pCMV-Myc (Takara Bio, Shiga, Japan) by replacing the sequence of the Myc tag and multicloning site with restriction enzyme sites of Xho I, Sal I and Not I. The resultant plasmids were designated pCXSN 6-LP CME and pCXSN Eg CME, respectively. For the construction of chimeric VLPs between 6-LP and Eg, a Sma I site was generated by substitution of t to c (in 6-LP) and a to g (in 6-LP and Eg) at nucleotide positions 460 and 463, respectively, of the prM gene by PCR. The sequence containing the prM gene (nucleotides 461-555) and E gene (nucleotides 1-1500) was digested by Sma I and Not I from pCXSN 6-LP CME or pCXSN Eg CME and inserted into pCXSN Eg CME or pCXSN 6-LP CME. The resultant plasmids were designated pCXSN Eg CM 6-LP E and pCXSN 6-LP CM Eg E, respectively. The constructs for single or double mutant VLPs were generated by PCR with pCXSN 6-LP CME or pCXSN Eg CME.
WNV replicon cDNA construct (pWNR NS1-5 EG2 AN), was generously provided by Dr. Peter W. Mason, University of Texas Medical Branch, USA . WNVR NS1-5 EG2 AN encodes the nonstructural proteins (NS1-5) of WNV 3356 strain isolated from American crow in 2000 , eGFP, autocatalytic foot-and mouth disease virus 2A protease and neomycin phosphotransferase II under the translational control of encephalomyocarditis virus internal ribosomal entry site. One μg of pWNR NS1-5 EG2 AN was linearized with Xba I and purified with a PCR purification kit (QIAGEN Inc), followed by ethanol precipitation. WNV replicon RNA was produced with in vitro transcription with an mMESSAGE mMASHINE T7 kit (Applied Biosystems) according to the manufacture's instructions. BHK cells (5 × 106) were trypsinized, washed three times with phosphate-buffered saline (PBS) and resuspended in 450 μl of PBS. Then, 5 μg of replicon RNA was added to the cell suspension and introduced by using a GenePulser II elecroporation apparatus (Bio-Rad Laboratories) at 750 V, 25 μF with the resistance set to ∞. Cells were cultured in 10 cm dishes with MEM supplemented with 10% FBS for 24 h. The culture media were replaced with Opti-MEM (Invitrogen) and incubated at 37°C for 30 min. The cells were transfected with 12 μg of the plasmid encoding the sequence of WNV structural genes by Lipofectamine (Invitrogen). After 48 h, supernatants were collected and cell debris was removed by centrifugation at 1000 g for 5 min. The supernatants were concentrated with Centriplus (Millipore). For the IFU assay, Vero cells in 24 well plates were infected with serial 10-fold dilutions of VLP preparations. After a 1 h incubation at 37°C, the solutions were removed and replaced with the culture media. After 48 h p.i., the number of VLPs-infected cells was counted by eGFP signals and the IFU value was calculated.
Monolayer cultures of HUVEC and transport assay of VLPs
HUVEC were seeded in transwell inserts for 24 well plates with polycarbonate membranes having 0.4 μm pores (Millipore). The media volumes were 200 μl for transwells and 700 μl for the lower chambers, respectively. The cells were cultured for 3 days and the integrity of tight junctions was evaluated by measuring TEER using a Millicell ERS (Millipore). The wells showing TEER elevation (more than 66 Ωcm2) were used for experiments. For VLPs transport assay, HUVEC were exposed to 4 × 104 IFU/transwell of VLPs (2 m.o.i.). The media in the lower chambers were collected at the indicated time points and subjected to the IFU assay on Vero cells.
Immunofluorescence of ZO-1
HUVEC seeded in transwells were exposed with 6-LP VLPs or treated with TNF-α. After 24 h, the cells were washed with PBS once and fixed with 4% paraformaldehyde (PFA) in PBS for 10 min at room temperature. After washing with PBS three times, the cells were permeabilized with 0.1% Triton X-100 in PBS and blocked with 2% bovine serum albumin in PBS (blocking solution) for 15 min at room temperature. The primary antibody incubation was performed overnight at 4°C with rabbit antiserum to human ZO-1 (BD Transduction Laboratories) diluted at 1:1000 in blocking solution. Then the cells were washed with PBS three times, and Alexa 488 conjugated donkey anti-rabbit IgG antibodies (Invitrogen) were added at 1:1000 dilution in blocking solution for a 1 h incubation at room temperature. After a PBS wash, the membranes were cut from transwell, placed on cover glasses and observed by fluorescent microscopy.
70k Dextran transfer assay
Fluorescein (FITC)-labeled 70k Dx (Invitrogen) was added into HUVEC with 6-LP VLPs, TNF-α (positive control) or media (negative control). After 24 h incubation at 37°C, 100 μl of medium was collected from each well and transferred into a 96-well plate. The FITC signal was read by a fluorescent plate reader, Mithras LB940 (Berthold). The relative transfer of 70k Dx was calculated by dividing the FITC signal of samples incubated with 6-LP VLPs or TNF-α by the mean of the signal of the negative control. The relative transfer of 70k Dx in the negative control was defined as 1.
Effect of endocytosis inhibitors on the transport of 6-LP VLPs
For stock solutions, chlorpromazine (Sigma) and filipin III (Sigma) were dissolved in dimethyl sulfoxide (DMSO) at 5 and 1 mg/ml, respectively. HUVEC in transwells were preincubated with the inhibitor at the indicated concentration for 30 min, and exposed to 6-LP VLPs for 24 h. For the control, DMSO was added in the media at concentration of 0.1%. The evaluation of the transported VLPs was performed as described above. The integrity of monolayer of HUVEC was confirmed by the 70k Dx transfer assay described above.
Western blotting for E protein
Wild type or mutant VLPs were produced with 293T cells as described above. Supernatants from cell cultures were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting with a mouse monoclonal antibody to WNV E protein clone 3.91 D (Millipore) for the primary antibody and horseradish peroxidase (HRP)-conjugated goat antibodies to mouse immunoglobulin (1:5,000 dilution; Biosource). The immunocomplex was visualized with Immobilon™ Western chemiluminescent HRP substrate (Millipore) and LAS-1000 mini (FIJIFILM, Tokyo, Japan).
Quantitative data are expressed as means ± standard deviation (SD) and were compared with Student's t test.
The authors gratefully acknowledge the invaluable suggestions by Dr. B. Caughey and Dr. C. D. Orrú, Rocky Mountain Laboratories, NIAID, NIH. The authors are grateful to Dr. P. W. Mason, University of Texas Medical Branch for WNV replicon cDNA construct. The authors acknowledge Dr. I. Takashima, Hokkaido University for providing WNV NY99 6-LP and Eg strains. The authors thank Ms. M. Sasada for technical assistance. This work was supported in part by Grant-in-Aids for young scientist B (R. H.), Scientific Research C (T. K.) and the Program of Founding Research Centers for Emerging and Reemerging Infectious Diseases (R. H., T. K. and H. S.) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
- Cernescu C, Ruta SM, Tardei G, Grancea C, Moldoveanu L, Spulbar E, Tsai T: A high number of severe neurologic clinical forms during an epidemic of West Nile virus infection. Rom J Virol. 1997, 48 (1-4): 13-25.PubMedGoogle Scholar
- Jamgaonkar AV, Yergolkar PN, Geevarghese G, Joshi GD, Joshi MV, Mishra AC: Serological evidence for Japanese encephalitis virus and West Nile virus infections in water frequenting and terrestrial wild birds in Kolar District, Karnataka State, India. A retrospective study. Acta Virol. 2003, 47 (3): 185-188.PubMedGoogle Scholar
- Malkinson M, Banet C, Weisman Y, Pokamunski S, King R, Drouet MT, Deubel V: Introduction of West Nile virus in the Middle East by migrating white storks. Emerg Infect Dis. 2002, 8 (4): 392-397. 10.3201/eid0804.010217.PubMed CentralView ArticlePubMedGoogle Scholar
- Murgue B, Zeller H, Deubel V: The ecology and epidemiology of West Nile virus in Africa, Europe and Asia. Curr Top Microbiol Immunol. 2002, 267: 195-221.PubMedGoogle Scholar
- Asnis DS, Conetta R, Teixeira AA, Waldman G, Sampson BA: The West Nile Virus outbreak of 1999 in New York: the Flushing Hospital experience. Clin Infect Dis. 2000, 30 (3): 413-418. 10.1086/313737.View ArticlePubMedGoogle Scholar
- Nash D, Mostashari F, Fine A, Miller J, O'Leary D, Murray K, Huang A, Rosenberg A, Greenberg A, Sherman M: The outbreak of West Nile virus infection in the New York City area in 1999. N Engl J Med. 2001, 344 (24): 1807-1814. 10.1056/NEJM200106143442401.View ArticlePubMedGoogle Scholar
- Trock SC, Meade BJ, Glaser AL, Ostlund EN, Lanciotti RS, Cropp BC, Kulasekera V, Kramer LD, Komar N: West Nile virus outbreak among horses in New York State, 1999 and 2000. Emerg Infect Dis. 2001, 7 (4): 745-747. 10.3201/eid0704.010427.PubMed CentralView ArticlePubMedGoogle Scholar
- Artsob H, Gubler DJ, Enria DA, Morales MA, Pupo M, Bunning ML, Dudley JP: West Nile Virus in the New World: Trends in the Spread and Proliferation of West Nile Virus in the Western Hemisphere. Zoonoses Public Health. 2009Google Scholar
- Lindsey NP, Kuhn S, Campbell GL, Hayes EB: West Nile virus neuroinvasive disease incidence in the United States, 2002-2006. Vector Borne Zoonotic Dis. 2008, 8 (1): 35-39. 10.1089/vbz.2007.0137.View ArticlePubMedGoogle Scholar
- Schneider BS, Soong L, Girard YA, Campbell G, Mason P, Higgs S: Potentiation of West Nile encephalitis by mosquito feeding. Viral Immunol. 2006, 19 (1): 74-82. 10.1089/vim.2006.19.74.View ArticlePubMedGoogle Scholar
- Sampson BA, Ambrosi C, Charlot A, Reiber K, Veress JF, Armbrustmacher V: The pathology of human West Nile Virus infection. Hum Pathol. 2000, 31 (5): 527-531. 10.1053/hp.2000.8047.View ArticlePubMedGoogle Scholar
- Khouzam RN: Significant cardiomyopathy secondary to West Nile virus infection. South Med J. 2009, 102 (5): 527-528.View ArticlePubMedGoogle Scholar
- Gupta M, Ghaffari M, Freire AX: Rhabdomyolysis in a patient with West Nile encephalitis and flaccid paralysis. Tenn Med. 2008, 101 (4): 45-47.PubMedGoogle Scholar
- Armah HB, Wang G, Omalu BI, Tesh RB, Gyure KA, Chute DJ, Smith RD, Dulai P, Vinters HV, Kleinschmidt-DeMasters BK: Systemic distribution of West Nile virus infection: postmortem immunohistochemical study of six cases. Brain Pathol. 2007, 17 (4): 354-362. 10.1111/j.1750-3639.2007.00080.x.View ArticlePubMedGoogle Scholar
- Shirato K, Kimura T, Mizutani T, Kariwa H, Takashima I: Different chemokine expression in lethal and non-lethal murine West Nile virus infection. J Med Virol. 2004, 74 (3): 507-513. 10.1002/jmv.20205.View ArticlePubMedGoogle Scholar
- Verma S, Lo Y, Chapagain M, Lum S, Kumar M, Gurjav U, Luo H, Nakatsuka A, Nerurkar VR: West Nile virus infection modulates human brain microvascular endothelial cells tight junction proteins and cell adhesion molecules: Transmigration across the in vitro blood-brain barrier. Virology. 2009, 385 (2): 425-433. 10.1016/j.virol.2008.11.047.PubMed CentralView ArticlePubMedGoogle Scholar
- Paddock CD, Nicholson WL, Bhatnagar J, Goldsmith CS, Greer PW, Hayes EB, Risko JA, Henderson C, Blackmore CG, Lanciotti RS: Fatal hemorrhagic fever caused by West Nile virus in the United States. Clin Infect Dis. 2006, 42 (11): 1527-1535. 10.1086/503841.View ArticlePubMedGoogle Scholar
- Scholle F, Girard YA, Zhao Q, Higgs S, Mason PW: trans-Packaged West Nile virus-like particles: infectious properties in vitro and in infected mosquito vectors. J Virol. 2004, 78 (21): 11605-11614. 10.1128/JVI.78.21.11605-11614.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- McKenzie JA, Ridley AJ: Roles of Rho/ROCK and MLCK in TNF-alpha-induced changes in endothelial morphology and permeability. J Cell Physiol. 2007, 213 (1): 221-228. 10.1002/jcp.21114.View ArticlePubMedGoogle Scholar
- Subtil A, Hemar A, Dautry-Varsat A: Rapid endocytosis of interleukin 2 receptors when clathrin-coated pit endocytosis is inhibited. J Cell Sci. 1994, 107 (Pt 12): 3461-3468.PubMedGoogle Scholar
- Orlandi PA, Fishman PH: Filipin-dependent inhibition of cholera toxin: evidence for toxin internalization and activation through caveolae-like domains. J Cell Biol. 1998, 141 (4): 905-915. 10.1083/jcb.141.4.905.PubMed CentralView ArticlePubMedGoogle Scholar
- Beasley DW, Barrett AD: Identification of neutralizing epitopes within structural domain III of the West Nile virus envelope protein. J Virol. 2002, 76 (24): 13097-13100. 10.1128/JVI.76.24.13097-13100.2002.PubMed CentralView ArticlePubMedGoogle Scholar
- Chu JH, Chiang CC, Ng ML: Immunization of flavivirus West Nile recombinant envelope domain III protein induced specific immune response and protection against West Nile virus infection. J Immunol. 2007, 178 (5): 2699-2705.View ArticlePubMedGoogle Scholar
- Chu JJ, Leong PW, Ng ML: Characterization of plasma membrane-associated proteins from Aedes albopictus mosquito (C6/36) cells that mediate West Nile virus binding and infection. Virology. 2005, 339 (2): 249-260. 10.1016/j.virol.2005.05.026.View ArticlePubMedGoogle Scholar
- Chu JJ, Ng ML: Interaction of West Nile virus with alpha v beta 3 integrin mediates virus entry into cells. J Biol Chem. 2004, 279 (52): 54533-54541. 10.1074/jbc.M410208200.View ArticlePubMedGoogle Scholar
- Chu JJ, Rajamanonmani R, Li J, Bhuvanakantham R, Lescar J, Ng ML: Inhibition of West Nile virus entry by using a recombinant domain III from the envelope glycoprotein. J Gen Virol. 2005, 86 (Pt 2): 405-412. 10.1099/vir.0.80411-0.View ArticlePubMedGoogle Scholar
- Lee JW, Chu JJ, Ng ML: Quantifying the specific binding between West Nile virus envelope domain III protein and the cellular receptor alphaVbeta3 integrin. J Biol Chem. 2006, 281 (3): 1352-1360. 10.1074/jbc.M506614200.View ArticlePubMedGoogle Scholar
- Li L, Barrett AD, Beasley DW: Differential expression of domain III neutralizing epitopes on the envelope proteins of West Nile virus strains. Virology. 2005, 335 (1): 99-105. 10.1016/j.virol.2005.02.011.View ArticlePubMedGoogle Scholar
- Chu JJ, Ng ML: Infectious entry of West Nile virus occurs through a clathrin-mediated endocytic pathway. J Virol. 2004, 78 (19): 10543-10555. 10.1128/JVI.78.19.10543-10555.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Medigeshi GR, Hirsch AJ, Streblow DN, Nikolich-Zugich J, Nelson JA: West Nile virus entry requires cholesterol-rich membrane microdomains and is independent of alphavbeta3 integrin. J Virol. 2008, 82 (11): 5212-5219. 10.1128/JVI.00008-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Beasley DW, Davis CT, Estrada-Franco J, Navarro-Lopez R, Campomanes-Cortes A, Tesh RB, Weaver SC, Barrett AD: Genome sequence and attenuating mutations in West Nile virus isolate from Mexico. Emerg Infect Dis. 2004, 10 (12): 2221-2224.PubMed CentralView ArticlePubMedGoogle Scholar
- Beasley DW, Li L, Suderman MT, Barrett AD: Mouse neuroinvasive phenotype of West Nile virus strains varies depending upon virus genotype. Virology. 2002, 296 (1): 17-23. 10.1006/viro.2002.1372.View ArticlePubMedGoogle Scholar
- Beasley DW, Whiteman MC, Zhang S, Huang CY, Schneider BS, Smith DR, Gromowski GD, Higgs S, Kinney RM, Barrett AD: Envelope protein glycosylation status influences mouse neuroinvasion phenotype of genetic lineage 1 West Nile virus strains. J Virol. 2005, 79 (13): 8339-8347. 10.1128/JVI.79.13.8339-8347.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Shirato K, Miyoshi H, Goto A, Ako Y, Ueki T, Kariwa H, Takashima I: Viral envelope protein glycosylation is a molecular determinant of the neuroinvasiveness of the New York strain of West Nile virus. J Gen Virol. 2004, 85 (Pt 12): 3637-3645. 10.1099/vir.0.80247-0.View ArticlePubMedGoogle Scholar
- Bazzoni G, Dejana E: Endothelial cell-to-cell junctions: molecular organization and role in vascular homeostasis. Physiol Rev. 2004, 84 (3): 869-901. 10.1152/physrev.00035.2003.View ArticlePubMedGoogle Scholar
- Huang SH, Jong AY: Cellular mechanisms of microbial proteins contributing to invasion of the blood-brain barrier. Cell Microbiol. 2001, 3 (5): 277-287. 10.1046/j.1462-5822.2001.00116.x.View ArticlePubMedGoogle Scholar
- Defazio G, Gelati M, Corsini E, Nico B, Dufour A, Massa G, Salmaggi A: In vitro modulation of adhesion molecules, adhesion phenomena, and fluid phase endocytosis on human umbilical vein endothelial cells and brain-derived microvascular endothelium by IFN-beta 1a. J Interferon Cytokine Res. 2001, 21 (5): 267-272. 10.1089/107999001300177448.View ArticlePubMedGoogle Scholar
- Kallmann BA, Wagner S, Hummel V, Buttmann M, Bayas A, Tonn JC, Rieckmann P: Characteristic gene expression profile of primary human cerebral endothelial cells. FASEB J. 2002, 16 (6): 589-591.PubMedGoogle Scholar
- Stins MF, Gilles F, Kim KS: Selective expression of adhesion molecules on human brain microvascular endothelial cells. J Neuroimmunol. 1997, 76 (1-2): 81-90. 10.1016/S0165-5728(97)00036-2.View ArticlePubMedGoogle Scholar
- Man S, Ubogu EE, Williams KA, Tucky B, Callahan MK, Ransohoff RM: Human brain microvascular endothelial cells and umbilical vein endothelial cells differentially facilitate leukocyte recruitment and utilize chemokines for T cell migration. Clin Dev Immunol. 2008, 2008: 384982-PubMed CentralView ArticlePubMedGoogle Scholar
- Shukaliak JA, Dorovini-Zis K: Expression of the beta-chemokines RANTES and MIP-1 beta by human brain microvessel endothelial cells in primary culture. J Neuropathol Exp Neurol. 2000, 59 (5): 339-352.PubMedGoogle Scholar
- Arjona A, Foellmer HG, Town T, Leng L, McDonald C, Wang T, Wong SJ, Montgomery RR, Fikrig E, Bucala R: Abrogation of macrophage migration inhibitory factor decreases West Nile virus lethality by limiting viral neuroinvasion. J Clin Invest. 2007, 117 (10): 3059-3066. 10.1172/JCI32218.PubMed CentralView ArticlePubMedGoogle Scholar
- Garcia-Tapia D, Hassett DE, Mitchell WJ, Johnson GC, Kleiboeker SB: West Nile virus encephalitis: sequential histopathological and immunological events in a murine model of infection. J Neurovirol. 2007, 13 (2): 130-138. 10.1080/13550280601187185.View ArticlePubMedGoogle Scholar
- Shrestha B, Ng T, Chu HJ, Noll M, Diamond MS: The relative contribution of antibody and CD8+ T cells to vaccine immunity against West Nile encephalitis virus. Vaccine. 2008, 26 (16): 2020-2033. 10.1016/j.vaccine.2008.02.009.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang T, Town T, Alexopoulou L, Anderson JF, Fikrig E, Flavell RA: Toll-like receptor 3 mediates West Nile virus entry into the brain causing lethal encephalitis. Nat Med. 2004, 10 (12): 1366-1373. 10.1038/nm1140.View ArticlePubMedGoogle Scholar
- Fiala M, Looney DJ, Stins M, Way DD, Zhang L, Gan X, Chiappelli F, Schweitzer ES, Shapshak P, Weinand M: TNF-alpha opens a paracellular route for HIV-1 invasion across the blood-brain barrier. Mol Med. 1997, 3 (8): 553-564.PubMed CentralPubMedGoogle Scholar
- Miller F, Fenart L, Landry V, Coisne C, Cecchelli R, Dehouck MP, Buee-Scherrer V: The MAP kinase pathway mediates transcytosis induced by TNF-alpha in an in vitro blood-brain barrier model. Eur J Neurosci. 2005, 22 (4): 835-844. 10.1111/j.1460-9568.2005.04273.x.View ArticlePubMedGoogle Scholar
- Yamshchikov G, Borisevich V, Seregin A, Chaporgina E, Mishina M, Michin V, Kwok CW, Yamshchikov V: An attenuated West Nile prototype virus is highly immunogenic and protects against the deadly NY99 strain: a candidate for live WN vaccine development. Virology. 2004, 330 (1): 304-312. 10.1016/j.virol.2004.09.014.View ArticlePubMedGoogle Scholar
- Chambers TJ, Halevy N, Nestorowicz A, Rice CM, Lustig S: West Nile virus envelope proteins: nucleotide sequence analysis of strains differing in mouse neuroinvasiveness. J Gen Virol. 1998, 79 (10): 2375-2380.View ArticlePubMedGoogle Scholar
- Halevy M, Akov Y, Ben-Nathan D, Kobiler D, Lachmi B, Lustig S: Loss of active neuroinvasiveness in attenuated strains of West Nile virus: pathogenicity in immunocompetent and SCID mice. Arch Virol. 1994, 137 (34): 355-70. 10.1007/BF01309481.View ArticlePubMedGoogle Scholar
- Nybakken GE, Nelson CA, Chen BR, Diamond MS, Fremont DH: Crystal structure of the West Nile virus envelope glycoprotein. J Virol. 2006, 80 (23): 11467-11474. 10.1128/JVI.01125-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Davis CW, Nguyen HY, Hanna SL, Sanchez MD, Doms RW, Pierson TC: West Nile virus discriminates between DC-SIGN and DC-SIGNR for cellular attachment and infection. J Virol. 2006, 80 (3): 1290-1301. 10.1128/JVI.80.3.1290-1301.2006.PubMed CentralView ArticlePubMedGoogle Scholar
- Shi PY, Tilgner M, Lo MK: Construction and characterization of subgenomic replicons of New York strain of West Nile virus. Virology. 2002, 296 (2): 219-233. 10.1006/viro.2002.1453.View ArticlePubMedGoogle Scholar
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