Figure 6

The role of SUR7 in endocytosis in C. albicans hyphal form. (A) Fluorescence microscopy was used to assess cellular localization of C. albicans Sur7p in the filamentous form of the SUR7-GFP strain. Hyphal growth was induced in RPMI-1640 medium at 37°C and protein localization was visualized at stages of early germination (top panel) and mature hyphae formation (bottom panel). Brightfield, green fluorescent, and merged images are shown. Sur7p-GFP is observed at the plasma membrane of the germinating tube, as is the case in yeast cells, but is absent from the growing hyphal tip. (B) FM4-64 was used to stain the vacuoles in C. albicans hyphae following standard protocols for vacuolar staining of the yeast cells [41].