Plasmid or strain | Description | Source or reference |
---|---|---|
Plasmids | Â | Â |
pCR2.1 | TA cloning vector, 3.9-kb, Ampr | Invitrogen |
pCRure 1 | pCR2.1 with 2.2-kb insert containing the B. suis ure 1D, ure 1A, ure 1B, ure 1C genes; Ampr | This study |
PCRure2 ABC | pCR2.1 with 2.2-kb insert containing the B. suis ure 2A, ure 2B, ure 2C genes; Ampr | This study |
pGEM-3Z | Cloning vector, 2.74-kb, Ampr | Promega |
pGEMure 1 | pGEM-3Z with 2.2-kb insert containing the B. suis ure 1D, ure 1A, ure 1B, and ure 1C genes; Ampr | This study |
pGEMure2 ABC | pGEM-3Z with 2.2-kb insert containing the B. suis ure 2A, ure 2B, and ure 2C genes; Ampr | This study |
pGEMure2 ABC-2 | pGEM-3Z with 2.9-kb insert containing the B. suis ure 2A, ure 2B, ure 2C genes; Ampr | This study |
pUC4K | Cloning vector, 3.9-kb, Kanr, Ampr | Pharmacia |
pGEMure1 K | pGEMure 1 with 0.9-kb Nco I fragment deleted and blunt ended and a 1.6-kb Pvu II-cut and blunt-ended Kanr cassette from pUC4K ligated, Kanr, Ampr | This study |
pGEMure2 ABCK | pGEMure2 with 0.6-kb Sac II fragment deleted and blunt ended and a 1.6-kb Pvu II-cut and blunt-ended Kanr cassette from pUC4K ligated, Kanr, Ampr | This study |
pGEMure2 ABCC | pGEMure2 with 1.2-kb Cla I and Mfe I fragment deleted and blunt ended and a 1.7-kb Eco52 I plus Kpn I-cut and blunt-ended Cmr cassette from pBBR4MCS ligated, Cmr, Ampr | This study |
pBBR4MCS | Broad-host-range vector; Cmr | [27] |
Strains | Â | Â |
Escherichia coli Top10 | Â | Â |
 | F- mcrA Δ(mrr-hsd RMS-mcr BC) Φ80lac ZΔM15 Δlac X74 deo Rrec A1 ara D139 Δ(ara-leu) 7697 gal U gal K rps L (StrR) end A1 nup G | Invitrogen |
Brucella suis | Â | Â |
1330 | Parent-type, smooth strain | G.G. Schurig |
1330Δure1 K | ure1 DA deleted mutant of 1330, Kanr | This study |
1330Δure2 K | ure2 BC deleted mutant of 1330, Kanr | This study |
1330Δure2 C | ure2 ABC deleted mutant of 1330, Cmr | This study |
1330Δure1 KΔure2C | ure1 DA and ure2 ABC deleted mutant of 1330, Cm r, Kanr | This study |