Figure 2
From: No detectable effect of RNA-binding protein Hfq absence in Staphylococcus aureus
Northern blot analysis of RNAIII (2A) or spa mRNA (2B) expression in S. aureus WT and Δhfq strains. Total RNA was extracted from post-exponential phase cultures of RN6390, Newman and COL, WT and Δhfq strains. 15 μg of total RNA of RN6390 and Newman strains were analysed by northern blot, using an RNAIII-specific probe (2A). Total RNA from RN6390, Newman and COL strains was also compared for spa mRNA expression, using a spa-specific probe (2B). Results from three independent experiments were identical.