Table 1 msp2 hypervariable region primers to determine newly identified msp2 paralogs in low passage A. phagocytophilum genomic DNA.
msp2 transcript locus | Forward primer sequence (name) | Reverse primer sequence (name) |
|---|---|---|
msp2 (pan-msp2) | 5'-TGATGTTGTTACTGGACAGA-3' (MSP465f) | 5'-CACCTAACCTTCATAAGAA-3' (MSP980r) |
RW1 | 5'-GCGACGAGGAAGGACAGTGGT-3' | 5'-AGTCTTCAACCCAGTCCCGCT-3' |
RW17 | 5'-GTGACGAAGAATTACGACAGT-3' | 5'-GCTAGAAGATCCGGTTCCTGT-3' |
RW20 | 5'-GTGACGAAGAAGGGGACAAAC-3' | 5'-GTTAGAGGCTCCGGTTCCTGT-3' |
RW23 | 5'-ACAAAGCGGAAGGATGGTGAT-3' | 5'-ATTCGATTAGACGTTTCACT-3' |