Targets | Assay | Name | Primers and probes |
---|---|---|---|
Mycoplasmas | |||
tuf genea) | real-time PCR | Mollicutes 414F | 5'-TCCAGGWCAYGCTGACTA-3' |
Mollicutes 541R | 5'-ATTTTWGGAACKCCWACTTG-3' | ||
Probe 451Fa) | 5'-GGTGCTGCACAAATGGATGG-3' | ||
tuf gene | Sequencing 1st | Myco-tuf-F1 | 5'-HATHGGCCAYRTTGAYCAYGGKAAAA-3' |
Myco-tuf-F2 | 5'-ATGATYACHGGDGCWGCHCAAATGGA-3' | ||
Sequencing 2nd | Myco-tuf-R1 | 5'-CCRCCTTCRCGRATDGAGAAYTT-3' | |
Myco-tuf-R2 | 5'-TKTRTGACGDCCACCTTCYTC-3' | ||
16s-23s rRNA intergenic spacer region | nested PCR 1st | MCGpF11 | 5'-ACACCATGGGAGYTGGTAAT-3' |
R23-1R | 5'-CTCCTAGTGCCAAGSCATYC-3' | ||
nested PCR 2nd | R16-2 | 5'-GTGSGGMTGGATCACCTCCT-3' | |
MCGpR21 | 5'-GCATCCACCAWAWACYCTT-3' | ||
Orientia tsutsugamushi | |||
47kDa common antigen coding gene | real-time PCR | Ots-47k-F | 5'-AATTCGTCGTGGTATGTTAAATG-3' |
Ots-47k-R | 5'-AGCAATTCCACATTGTGCTG-3' | ||
Ots-47k-P b) | 5'-TGCTTAATGAATTAACTCCAGAATT-3' |