Table 3 Bacterial strains, plasmids and primers used in this study
From: Members of the Sinorhizobium meliloti ChvI regulon identified by a DNA binding screen
Strain/Plasmid/Primer | Relevant characteristics | Reference or source |
|---|---|---|
Sinorhizobium meliloti | ||
Rm1021 | SU47 str-21, Smr wild type | |
SmUW38 | Rm1021 chvI::nptII (chvI261) | [10] |
SmFL430 | RmP110 SMa2295::pTH1522 | [20] |
SmFL112 | RmP110 msbA2::pTH1522 | [20] |
SmFL4665 | RmP110 SMb21189::pTH1522 | [20] |
SmFL5401 | RmP110 SMb21190::pTH1522 | [20] |
SmFL918 | RmP110 SMc00262::pTH1522 | [20] |
SmFL4392 | RmP110 SMc00261::pTH1522 | [20] |
SmFL2950 | RmP110 rhbB::pTH1522 | [20] |
SmFL5628 | RmP110 rhtX::pTH1522 | [20] |
SmFL5755 | RmP110 rhbF::pTH1522 | [20] |
SmUW45 | Rm1021 SMa2295::pTH1522 | This study |
SmUW43 | Rm1021 msbA2::pTH1522 | This study |
SmUW58 | Rm1021 SMb21189::pTH1522 | This study |
SmUW59 | Rm1021 SMb21190::pTH1522 | This study |
SmUW46 | Rm1021 SMc00262::pTH1522 | This study |
SmUW57 | Rm1021 SMc00261::pTH1522 | This study |
SmUW55 | Rm1021 rhbB::pTH1522 | This study |
SmUW62 | Rm1021 rhtX::pTH1522 | This study |
SmUW63 | Rm1021 rhbF::pTH1522 | This study |
SmUW157 | SmUW38 SMa2295::pTH1522 | This study |
SmUW133 | SmUW38 msbA2::pTH1522 | This study |
SmUW148 | SmUW38 SMb21189::pTH1522 | This study |
SmUW149 | SmUW38 SMb21190::pTH1522 | This study |
SmUW136 | SmUW38 SMc00262::pTH1522 | This study |
SmUW147 | SmUW38 SMc00261::pTH1522 | This study |
SmUW145 | SmUW38 rhbB::pTH1522 | This study |
SmUW152 | SmUW38 rhtX::pTH1522 | This study |
SmUW153 | SmUW38 rhbF::pTH1522 | This study |
Escherichia coli | ||
DH5α | F-, φ80dlacZ∆M15, endA1, recA1∆(lacZYA-argF)U169, hsdR17(rK- mK+), deoR, thi-1, supE44, λ-, gyrA96, relA1 | [48] |
BL21(DE3)pLysS | F−, ompT, hsdSB (rB-, mB-), dcm, gal, λ(DE3), pLysS, Cmr | [49] |
Plasmid | ||
pGEM®-T Easy | Cloning of PCR products, Ampr | Promega (USA) |
pET-30a(+) | His•Tag expression vector, Kmr | EMD Chemicals (Novagen) |
pLB010 | pGEM®-T Easy::chvI, Ampr | This study |
pJF011 | pET-30a(+)::chvI, Kmr | This study |
pUC18 | Cloning vector, Ampr | [50] |
pTC198 | pUC19::5-kb chvI-exoShrpK, Ampr | [10] |
pTC190 | pVK101::4kb A. tumefaciens chvI/G, Tcr Kmr | [4] |
pKNG101 | sacB+mobRK2 oriR6K, Smr | [51] |
pKD001 | pTC190::pKNG101, Tcr | This study |
Primer | Sequence ( 5′-3′ ) | |
LB5 | atgcagaccatcgcgctt | This study |
LB6 | acatcgtgatccaacaagg | This study |
LB61 | gtaaaacgacggccagt | This study |