Members of the Sinorhizobium meliloti ChvI regulon identified by a DNA binding screen
© Bélanger and Charles; licensee BioMed Central Ltd. 2013
Received: 9 October 2012
Accepted: 8 February 2013
Published: 13 June 2013
The Sinorhizobium meliloti ExoS/ChvI two component regulatory system is required for N2-fixing symbiosis and exopolysaccharide synthesis. Orthologous systems are present in other Alphaproteobacteria, and in many instances have been shown to be necessary for normal interactions with corresponding eukaryotic hosts. Only a few transcriptional regulation targets have been determined, and as a result there is limited understanding of the mechanisms that are controlled by the system.
In an attempt to better define the members of the regulon, we have applied a simple in vitro electrophoretic screen for DNA fragments that are bound by the ChvI response regulator protein. Several putative transcriptional targets were identified and three were further examined by reporter gene fusion experiments for transcriptional regulation. Two were confirmed to be repressed by ChvI, while one was activated by ChvI.
Our results suggest a role for ChvI as both a direct activator and repressor of transcription. The identities and functions of many of these genes suggest explanations for some aspects of the pleiotropic phenotype of exoS and chvI mutants. This work paves the way for in depth characterization of the ExoS/ChvI regulon and its potential role in directing bacteria-host relationships.
KeywordsRhizobia Response regulator DNA-binding Exopolysaccharide Alfalfa nodulation chvI regulon Signal transduction Transcriptional regulation DNA binding assay
Two-component regulatory systems (TCRS) are the most abundant and widespread transcriptional regulators in bacteria, as indicated by the number of instances of the Pfam PF00072 response regulator receiver domain . Bacterial genomes typically contain dozens to hundreds of these systems . Response regulator domains of transcriptional regulatory proteins are phosphorylated by cognate sensor histidine kinase proteins in response to changes in environment or growth conditions . This phosphorylation results in conformational change of the response regulator protein, leading to transcriptional activation or repression. Even with the recognized importance of these systems, very few of them have been characterized with regard to the signal input and the regulatory targets.
The ExoS/ChvI two-component regulatory system, consisting of the membrane-spanning histidine protein kinase ExoS and the response regulator ChvI, is found in alphaproteobacterial genomes. In Agrobacterium tumefaciens, the ChvG/ChvI system is vital for plant tumor formation, and mutants are sensitive to acidic pH and detergents . The BvrS/BvrR system of Brucella abortus is required for virulence  and has a broad impact on cell envelope as well as carbon and nitrogen metabolism . The Bartonella henselae BatR/BatS system is also involved in regulating virulence-associated genes . Analysis of a mutant of the ExoS homolog of Rhizobium leguminosarum confirmed its requirement for successful nodule invasion and nitrogen fixation . This mutant also had a destabilized outer membrane, associated with reduction of ropB expression, as well as increased accumulation of intracellular poly-3-hydroxybutrate (PHB), and reduction in exopolysaccharide production. In all cases studied, ExoS/ChvI TCRS and its orthologs play a role, although not well understood, in the bacterial-host interaction.
Sinorhizobium meliloti exoS was first identified through a Tn5 insertion mutant that resulted in overproduction of exopolysaccharide due to disruption of the membrane-spanning portion of the protein, causing constitutive activation of the kinase activity, thus resulting in constant phosphorylation of ChvI . Null mutants of exoS and chvI are able to trigger the formation of nodules, but those nodules do not develop normally and they do not fix nitrogen . The mutants do not grow on complex or in liquid media, and cultivation on defined agar-media is challenging, a condition that prompted an early conclusion that exoS and chvI are essential for S. meliloti viability . A chvI deletion mutant demonstrated enhanced motility, and reduction in PHB accumulation, the opposite of what was found for a R. leguminosarum exoS homolog mutant . Similar to the R. leguminosarum mutant , alterations in LPS were observed. Also isolated in the Tn5 screen that yielded the constitutively activated exopolysaccharide overproducing exoS mutant was a mutant of exoR. Evidence has been provided to suggest a direct interaction of ExoR with ExoS in the periplasm, with ExoR binding contributing to the maintenance of ExoS in an inactive conformation . Furthermore, it has been proposed that cleavage of ExoR is induced by some yet unknown environmental signal during infection of the host plant, and this might modulate its ability to bind ExoS , resulting in its activation and regulation of the target genes.
The exoS gene is situated within an operon along with hprK, part of an incomplete phosphotransferase system (PTS) in Alphaproteobacteria. In S. meliloti, HprK is involved in succinate mediated catabolite repression . The establishment of a direct functional or regulatory link between the incomplete PTS and the ExoS/ChvI TCRS has been elusive, partly because the systems have often been studied in isolation.
Given the pleotropic nature of the exoS and chvI null mutants , investigation of gene expression using transcriptomics and proteomics might prove less than satisfactory, as the expression of many genes that are not direct regulatory targets is likely to be altered due to physiological changes in the cell. Indeed transcriptomics have identified hundreds of genes whose expression is affected by the exoS96::Tn5 mutation . Comparison of transcriptomes from two different chvI mutant strains (gain-of-function versus reduced-function) narrowed the set of genes regulated by ChvI and subsequently facilitated the identification by gel shift assays of three intergenic regions binding ChvI  and the determination of an 11-bp-long putative ChvI binding motif. However, for the majority of genes identified as being differentially expressed in a ChvI dependent manner in that study, including the succinoglycan synthesis genes, no binding to upstream regions could be demonstrated. As an alternative, we applied a method to screen for DNA fragments that were directly bound by the ChvI transcriptional regulator. Analysis of these targets suggests important metabolic pathways affected by ChvI regulation. In return, these new findings directed us to uncover better conditions for cultivation of the loss-of-function chvI mutants. Further analyses with reporter gene fusion assays confirmed the direct role of ChvI as a repressor for the rhizobactin and SMc00261 operons. It also confirmed the previously discovered direct activation of the msbA2 operon by ChvI. Methods developed here to identify ChvI targets have proved to be efficient and could be applied to other response regulators.
Application of electrophoretic mobility shift assay to the identification of ChvI-regulated genes
To better understand the role of ChvI as a response regulator, it is necessary to identify genes whose transcription is directly influenced by ChvI. To identify specific DNA sequences from plasmid or genomic DNA for which ChvI might have binding affinity, we adapted a method using the electrophoretic mobility shift principle . The plasmid DNA electrophoretic mobility shift assay (PD.EMSA) and genomic DNA electrophoretic mobility shift assay (GD.EMSA) methods involve incubation of purified DNA-binding protein with fractionated DNA, followed by electrophoresis through a native polyacrylamide gel using sodium boric acid (SB) buffer. In this study, the restriction endonuclease Bsp143I was used for DNA fragmentation. The use of SB buffer, a low conductivity medium, and a 14-cm gel as well as running the gel for 3–6 hours at low voltage, allowed unbound DNA fragments to migrate far from the top of the gel while ChvI-bound fragments remained near the wells (see Additional file 1). Inclusion of EDTA in the buffer resulted in no retardation of electrophoretic mobility suggesting an involvement of the putative Mg2+ site for ChvI-DNA interaction (see Additional file 2). The slower migrating bands were excised from the gel, purified, and cloned into pUC18 vector from which the insert DNA could be sequenced from each end to determine the extent of each fragment.
Bsp143I-digested pTC198 plasmid DNA was used to perform PD.EMSA (see Additional file 1). This pUC19 clone contains a 5-kb KpnI-fragment from S. meliloti Rm1021 spanning across the entire chvI-hprK genomic sequence including the intergenic region between pckA and chvI. This plasmid was employed to optimize the method with a smaller number of fragments than with genomic DNA, thus providing a better resolution on the gel but also increasing the chances of binding to areas surrounding chvI and exoS to test for possible autoregulation of ExoS/ChvI. Regulation of the adjacent gene pckA by chvG-chvI has been previously shown for A. tumefaciens using reporter gene fusion assays , therefore this experiment was also aimed at testing if S. meliloti ChvI could bind upstream of pckA.
Following the excision of electrophoretic bands from PD.EMSA of pTC198, DNA fragments were cloned into BamHI-linearized pUC18 and sequenced from both ends. Out of four inserts sequenced, three represent a 176-bp fragment (genomic origin from 48523 to 48699) coding for the region upstream of SMc02753, including its start codon. A single clone contained a 395-bp region spanning the upstream sequence of chvI and past the translational start site (genomic origin from 51887 to 52281). These results suggest that ChvI might autoregulate its transcription but most importantly, it shows a direct binding affinity between the ChvI and the upstream sequence of manXhpr operon part of the PTS system. The ChvI binding to the 176-bp fragment was also confirmed by performing a gel shift assay using a PCR-amplified DNA fragment from pLB102 and the purified ChvI protein (data not shown). Further delineation of this binding was not performed.
DNA fragments recovered from GD.EMSA and genes potentially regulated by ChvI
probable imidazoleglycerol-phosphate dehydratase
probable Na(+)/H(+)-antiporter (upstream mucR)
(upstream lpsL and rkpK)
ABC transporter ATP-binding transmembrane protein (upstream psd and pssA)
putative cardiolipin synthetase transmembrane protein (downstream exoR; upstream xthA2)
putative 3-ketoacyl-CoA thiolase
methionine import ATP-binding protein
putative dipeptidase (upstream ABC transporter)
inter & intra
lactose ABC transporter, permease component
putative site-specific recombinase
putative dipeptide ABC transporter permease and ATP-binding protein
putative acyltransferase (msbA2 operon)
intra & inter
SMb21151 /SMb21552 (aacC4)
putative aminoglycoside 6′-N-acetyltransferase
putative maltodextrin α-D-glucosyltransferase
thiamine biosynthesis protein
Potential functions of identified ChvI-regulated genes
Genes potentially regulated by ChvI are of diverse function (Table 1). Because DNA fragments binding ChvI are often found within a coding sequence and not in intergenic areas, it is difficult to predict if ChvI acts as an activator of an adjacent gene or a repressor of the gene it binds within. In several cases, such as the rhizobactin gene cluster and the msbA2 gene cluster, the ChvI-binding fragment is found in the first gene of what is predicted to be an operon. Table 1 lists genes found closest to a ChvI-binding DNA fragment but it is possible in many instances that genes further downstream could be part of the same transcript and also be ChvI-regulated. It is also important to note that the sequenced fragments are a subset of cloned fragments and other ChvI targets likely exist. Using the list of potentially ChvI-regulated genes obtained, we queried databases for functional relationships between targets: MetaCyc , KEGG  and STRING 8.1 . Based on these analyses, a number of functional linkages may be made between some potential ChvI targets.
Two fragments (F15 and F6) are linked to lactose catabolism. One is found in front of the lacFGZ1K gene cluster and the second is found in SMc00589 (a conserved hypothetical protein), about 300 bp upstream of gal (Smc00588). The lacFGZ1K gene cluster encodes genes for lactose ABC-transporter and a β-galactosidase (E.C. 22.214.171.124). β-D-galactose is degraded through the De Ley-Doudoroff pathway in S. meliloti[24, 25] and gal codes for the galactose dehydrogenase (EC 126.96.36.199) of this pathway.
Two other fragments (F7 and F5) suggest that ChvI is involved in regulating phospholipid biosynthesis. One fragment is found in SMc02076 (cls) and another one is found in SMc00550, about 300 bp upstream of psd (SMc00551) and followed by pssA (SMc00552). Cardiolipin is produced in S. meliloti and the only gene coding for a cardiolipin synthetase is cls. Interestingly, this gene is located about 1 kb downstream of the exoS-associated gene exoR. Proteins encoded by psd (phosphatidylcholine decarboxylase) and pssA (phosphatidylserine synthase) are responsible for the biosynthesis of phosphatidylethanolamine and phosphatidylserine respectively, and both of these phospholipids are also intermediates for phosphatidylcholine biosynthesis . Mutants of these genes exhibit deficiencies in alfalfa symbiosis . Aside from phospholipids synthesis, another link was found between SMc00550 and msbA2 using STRING 8.1. These two genes are homologs and might have similar functions. The fragment F8 found in SMc00262, a putative 3-ketoacyl-CoA thiolase, followed by SMc00261, a putative fatty-acid-CoA ligase, also suggests regulation of lipid metabolism. These genes are putatively involved in fatty acid β-oxidation.
ChvI was also found to bind fragments from genes involved in peptide and methionine transport. A fragment (F13) belongs to the upstream sequence of SMc03267 and four genes encoding a putative dipeptidase and a putative dipeptide ABC-type transporter. Another fragment (F19) is from SMb20478, part of a gene cluster coding for another dipeptide ABC-transporter. MetN involved in importing methionine also has a fragment of its gene having affinity for ChvI.
Growth rate constants of chvI261 mutant strain grown in MM9-succinate liquid media and with the addition of uracil and/or proline to the growth media
Addition to medium
0.182 ± 0.004
0.043 ± 0.003
0.167 ± 0.006
0.144 ± 0.004
uracil and proline
0.192 ± 0.003
0.161 ± 0.002
0.201 ± 0.014
0.159 ± 0.025
Confirmation of ChvI involvement in transcriptional regulation of identified target genes
Having identified genes that might be regulated by ChvI and conditions allowing the growth of the chvI mutant strain in liquid media, we used strains from a S. meliloti fusion library  to confirm the regulation at transcriptional levels. The library had been constructed using a vector that forms gene fusions to the reporter genes gfp+/lacZ or gusA/tdimer2(12) depending on the orientation of the insert. Because of the possible involvement of ChvI in regulating the S. meliloti lac operon, we selected gusA fusion strains to measure transcriptional activity using the β-glucuronidase assay. Gene fusions were transduced into chvI mutant SmUW38 and into the wild-type strain Rm1021, and then assayed for β-glucuronidase activity and compared. These assays have been applied to three operons identified by the DNA binding assays, confirming the regulation of all three operons by ChvI, and also demonstrating that ChvI can function as either an activator or a repressor, depending on the target gene. The transcription assay with a housekeeping gene in the two genetic backgrounds (wild-type versus chvI261) was not tested. However, we did examine expression of the gene SMa2295 with a fusion upstream of the ChvI binding site and the results showed low and not significant GusA activity difference between the two genotype backgrounds (23 versus 30 Miller Units).
The high basal level of the negatively regulated operons is not really unexpected given that we do not know the repressing conditions, and also the likelihood of multiple regulatory systems acting on these genes. These experiments involved the comparison of gene expression in genetic backgrounds that resulted in differences only in the presence / absence of the ChvI regulator. Otherwise, the environmental conditions were not altered.
An adaptation of methods to perform gel electrophoresis mobility shift assays allowed us to identify DNA fragments with higher affinity for ChvI. Analyses of these results force us to revise our earlier perceptions following phenotypic analyses of ExoS/ChvI as mainly a regulatory system for exopolysaccharide production. Our results suggest that the ChvI regulon includes genes from diverse pathways. Moreover, ChvI appears to have a dual regulatory role, activating and repressing different operons. The total number of targets likely far outnumbers the 27 fragments that we pulled out in our screen, especially considering that we did not hit the same fragment more than once, and we also did not find a few other targets that had previously been shown to be bound by ChvI.
The approach used in our study is highly complementary to the microarray and directed DNA binding study of Chen et al.  that resulted in the identification of several potential regulatory targets of ExoS/ChvI and the prediction of a consensus binding sequence. It is important to note, however, that of 19 upstream regions tested, binding was only detected to three (ropB1, SMb21440, SMc01580), and a putative consensus sequence was determined using some upstream regions to which binding had not been demonstrated. Confirmation of this consensus binding sequence awaits more detailed DNA footprinting experiments on a larger number of identified targets. It is possible that many ChvI-repressed genes may not have been detected in that study due to the use of a constitutively activated variant of the ChvI protein that might not have been able to function as a repressor.
The binding of ChvI within SMa2337 (rhtX) to repress rhtXrhbABCDEF gene transcription could suggest that following the sensing of a signal other than the presence of iron, ExoS/ChvI represses genes for rhizobactin 1021 production. This operon is known to be upregulated by RhrA in iron-depleted conditions  and downregulated by RirA in iron-replete conditions . Our fusion assay results confirmed that the rhizobactin operon is highly expressed in M9-minimal media (iron-depleted conditions). However, this expression is even higher in strains with the chvI null mutation. Iron is an important micronutrient found in many cofactors required for cytochrome and nitrogenase activity. Its acquisition however is difficult for two main reasons. First, it is poorly soluble at pH 7, and secondly, a high concentration of iron can cause the generation of hydroxy radicals. Bacteria produce siderophores to scavenge iron and therefore control iron availability. A tight control over the production of siderophore is thus important. The lack or the overproduction of rhizobactin 1021 by S. meliloti impairs the symbiotic relationship with alfalfa . Mutation of rirA derepresses rhizobactin production and as a result causes a growth defect of the strain relative to the presence of iron . The reduced viability of the rirA mutant due to oxidative stress suggested that perhaps this strain would also be affected in its symbiotic properties but it was not the case . This study suggested that in planta another unknown regulatory system might control the production of rhizobactin. Whether ExoS/ChvI might be the system responsible awaits further investigation.
Another important finding is the confirmation that ChvI is involved in activation of the expression of SMb21189, SMb21190, and msbA2. These genes have only been described recently in the literature although msbA2 in particular may play an important but incompletely defined role in symbiosis [34, 35], and the operon has already been shown to be subject to ChvI regulation . SMb21189 and SMb21190 encode glycosyltransferases and msbA2 is part of an ABC-transporter family involved in macromolecule export. The above mentioned recent studies proposed that the operon including SMb21188, a putative acyltransferase, is involved in the production and export of an unknown polysaccharide which uses intermediates from the succinoglycan production pathway. The regulation of this operon by ExoS/ChvI is therefore the closest link to the succinoglycan-deficient phenotype of exoS and chvI mutant strains. Although this ChvI-regulated operon is not required for succinoglycan production it seems to be functionally related to succinoglycan production.
The third operon that we confirmed to be differentially regulated by ChvI encodes proteins putatively involved in fatty acid β-oxidation. SMc00262 putatively produces a 3-ketoacyl-CoA and SMc00261, a fatty-acid-CoA ligase. These genes are also followed by SMc00260 coding for a putative short-chain dehydrogenase and SMc00259 coding for a hypothetical protein. Upstream of these genes lay genes for a transcriptional regulator of the IclR family (SMc00263) and another short-chain dehydrogenase (SMc00264). Our earlier studies failed to demonstrate a phenotype for SMc00260 and SMc00264 mutants . A tripartite ATP-independent periplasmic (TRAP) transporter system upregulated by the presence of acetoacetate and 3-methyl oxovaleric acid is encoded by genes in the same orientation upstream of SMc00264 . All these genes are organized in the same orientation and close enough to each other to be part of the same transcript. However, our finding of a ChvI binding site in SMc00262, after the gene encoding the IclR regulator, suggests a complex regulation of these genes. In fact, a N-Acyl homoserine lactone (AHL) also impacts on their expression . The fatty-acid-CoA ligase (SMc00261) has been found differentially accumulated in early log phase cultures of S. meliloti Rm1021 treated for 2 hours with 3-oxo-C16:1-HL while the periplasmic binding protein (SMc00265) accumulated in stationary phase cultures independently of the presence of AHLs. Perhaps under conditions that activate ChvI, the first part of the gene cluster is upregulated to allow the import of an organic acid but the second part responsible for its degradation and entry in the TCA cycle is downregulated. This hypothesis would suggest the use of this organic acid, under certain conditions, as a readily available building block rather than an energy source.
An important finding from this work is that uracil and proline improved the growth of the chvI mutant. This finding now allows us to culture the mutant strain in liquid media, greatly facilitating experimental analysis. Binding of ChvI in thiC (SMb20615) and in hisB (SMc02574), perhaps to repress the thiamine and histidine biosynthesis operons, made us hypothesize that a derepression of these operons in exoS or chvI mutants could lead to a deficiency in UTP formation and could explain the pleiotropy of these mutants. Rhizobial purine and pyrimidine auxotrophic mutants have been found affected in polysaccharides synthesis and plant invasion [39–42]. Further work needs to be done to confirm that chvI mutant auxotrophy is truly caused by a derepression of operons for thiamine and histidine biosynthesis.
We have identified a number of putative direct targets of ChvI, many of which are consistent with the pleotropic phenotype of exoS and chvI mutants. We also demonstrated that ChvI may act as a repressor or activator of gene expression, and surprisingly ChvI seems to often bind within predicted protein coding sequences. The bias is often to only consider intergenic regions for locations of potential regulatory sites. However, we note that the Fur regulator of Helicobacter pylori is just one example of a transcriptional regulatory protein that has targets within polycistronic operons and acts as a repressor and an activator of gene expression . The tendency to search for transcriptional cis-regulatory elements in intergenic areas rather than considering equally regions internal to ORFs may need to be revisited. GD.EMSA or Chromatin-Immunoprecipitation (ChIP) techniques are examples of techniques that do not have a bias towards intergenic or intragenic areas and their usage certainly make important contributions to our knowledge about transcriptional regulation.
Although this study has uncovered new facets of the ExoS/ChvI regulation, the regulatory signal is still unknown. However, a number of new hypotheses emerge. Several genes identified in this study as possibly ChvI-regulated are involved in amino acid and peptide metabolism and transport. In Rhizobium leguminosarum bv. viciae VF39SM, peptides have been shown to increase the expression of the outer membrane protein ropB in a ChvG-dependent manner . Perhaps ExoS and/or ExoR sense(s) peptides. Also, our work suggests a direct regulatory link between the PTS and the ExoS/ChvI systems; do these systems partner to coordinate the C and N metabolism as suggested by recent work in B. melitensis? With several putative ChvI-targeted genes now identified, tools necessary to test these hypotheses are available. We are cognizant, however, of the fact that our screen was not saturating, and we will endeavor to adapt the method for higher throughput so that we have a better understanding of the complete ChvI regulon and the nature of the ChvI consensus binding sequence.
Bacterial strains, plasmids and growth conditions
Bacterial strains, plasmids and primers used in this study
Reference or source
SU47 str-21, Smr wild type
Rm1021 chvI::nptII (chvI261)
F-, φ80dlacZ∆M15, endA1, recA1∆(lacZYA-argF)U169, hsdR17(rK- mK+), deoR, thi-1, supE44, λ-, gyrA96, relA1
F−, ompT, hsdSB (rB-, mB-), dcm, gal, λ(DE3), pLysS, Cmr
Cloning of PCR products, Ampr
His•Tag expression vector, Kmr
EMD Chemicals (Novagen)
pGEM®-T Easy::chvI, Ampr
Cloning vector, Ampr
pUC19::5-kb chvI-exoShrpK, Ampr
pVK101::4kb A. tumefaciens chvI/G, Tcr Kmr
sacB+mobRK2 oriR6K, Smr
Sequence ( 5′-3′ )
Cloning of chvI for His•Tag-ChvI expression and purification
S. meliloti Rm1021 chvI was PCR amplified using primers LB5 and LB6 (Table 3). The 800-bp PCR fragment was gel-purified and then cloned in pGEM®-T Easy vector. Plasmid pLB010 with the insert in the correct orientation for expression was verified by DNA sequence analysis. NotI chvI-containing fragment was then cut out of pLB010 and ligated to NotI-digested pET-30a, generating a N-terminal His•Tag fusion pJF011. E. coli BL21(DE3)pLysS clones carrying the pJF011 plasmid were confirmed for His•Tag-ChvI production by western blot using a His•Tag monoclonal antibody from mouse (Novagen) and Alexa Fluor 488 goat anti-mouse IgG (H + L) (Invitrogen, Molecular Probes) as the secondary antibody. His•Tag-ChvI purification using nickel-affinity chromatography was performed in the laboratory of Professor Bi-Cheng Wang at University of Georgia (USA).
Electrophoretic mobility shift assay using genomic DNA (GD.EMSA)
To prepare samples, S. meliloti Rm1021 genomic DNA was digested to completion by overnight incubation with Bsp143I restriction enzyme (Sau3AI isoschizomer, Fermentas Life Sciences, Canada) and the reaction was then heat-inactivated. Purified His•Tag-ChvI protein was mixed with digested DNA in a solution of 9% glycerol, 3 mM acetyl phosphate, 0.8 mM Tris-acetate, 0.25 mM magnesium acetate, 1.65 mM potassium acetate, 2.5 μg ml-1 bovine serum albumin (BSA). For negative controls, ChvI protein was not added to samples. Incubations were carried out for 30 minutes at room temperature and loaded directly on gel without dye.
To perform the electrophoresis, a sodium boric acid buffer (SB buffer) was made following the specifications of Brody and Kern . 5% nondenaturing polyacrylamide gels (14 cm × 16 cm) were cast using a Hoefer SE 600 gel electrophoresis unit and following the standard procedure for resolution of small DNA fragments  but using SB buffer instead of TBE buffer. Gels were run in 1X SB buffer between 25 to 40 mA for 3–6 hours. Gels were then stained for 1 hour in a 3X GelRed™ staining solution containing 0.1 M NaCl and following manufacturer’s recommendation for post gel staining (Biotium, USA, CA) prior to visualization on a UV transilluminator. Shifted DNA bands in the highest part of the gel were then excised and stored in 2-ml plastic tubes at −20°C.
To recover DNA fragments from polyacrylamide gel, the method from Ausubel et al. (1992)  was used. The elution buffer used contained 0.5 M ammonium acetate, 1 mM EDTA, 0.1% SDS and final pH 8. 200 μl elution buffer was added to each tube containing a piece of gel. The gel was then crushed in smaller pieces using a pipet tip. Tubes were incubated overnight at 37°C with shaking. Following centrifugation in a microcentrifuge at room temperature for 10 minutes at 10,000 rpm, supernatant was removed and transferred to a clean 2.0 ml tube. Ethanol (500 μl) was added to precipitate the DNA and tubes were placed at −20°C overnight. DNA was pelleted at 13,000 rpm for 10 minutes. Supernatant was removed and DNA solubilized in 100 μl of 10 mM Tris pH 8 and 15 μl of 5 M sodium chloride was added. DNA was then precipitated a second time with 2 volumes of ethanol and kept overnight at −20°C. Precipitated DNA was recovered by centrifugation in a microcentrifuge at 13,000 rpm for 15 minutes, supernatant was removed and DNA was dried. Final resuspension of DNA was done with 10 μl of 10 mM Tris pH 8.
The DNA fragments were cloned into the BamHI site in pUC18. Prior to ligation, BamHI-digested pUC18 was dephosphorylated using shrimp alkaline phosphatase (Fermentas Inc.) and the reaction stopped by heat-inactivation. Ligation was performed overnight at room temperature with T4 DNA ligase (Fermentas Inc.). Transformation of calcium chloride competent E. coli DH5α cells was done following standard procedure . Over 40 transformant colonies were streak-purified from each experiment. A selection of them were then used for plasmid preparation and tested for the presence of an insert using restriction digest with EcoRI and PstI. Fragments cloned in pUC18 were sequenced using primers M13F provided by the sequencing facility (University of Waterloo) or LB61 (Table 3).
Sequences were first analyzed by searching for Sau3AI (Bsp143I) restriction sites to determine the limits of each fragment. Each fragment sequence was then searched against S. meliloti Rm1021 genomic sequence using the BLAST tool from Toulouse annotation website . Genes in closest proximity to identified sequences and potentially regulated by ChvI were searched against STRING 8.1 databases (June 28, 2009) for functional relations . The search was directed from the Toulouse annotation website.
Reporter gene fusion strains
Transcriptional fusion strains were obtained by transduction from the reporter gene fusion library strains made by Cowie et al. . SmFL strains were used to prepare transduction lysates to transfer the gene fusions from the original S. meliloti RmP110 background into the Rm1021 background. Selection of transductants was done on LB with gentamicin (60 μg ml-1). The same lysates were also used to transduce gene fusions into SmUW38 (pKD001) with selection on LB gentamicin (60 μg ml-1) and neomycin (200 μg ml-1). Four transductants per transduction experiment were picked and streaked on LB gentamicin and neomycin. Transductants were then cured of pKD001 by streaking them on MM9-succinate gentamicin (20 μg ml-1) containing 2.5% sucrose and incubated at 30°C for four days. pDK001-cured strains were finally streaked on MM9-succinate gentamicin.
Phage ΦM12 was used for transductions following the usual procedure , except that TY media was used instead of LBmc media to prepare and dilute lysates. High yield of transductants required the use of Bacto™-Agar, -Tryptone, and -Yeast extract (BD). Diluted lysate (0.5 ml) was mixed with equal volume of cell suspension and incubated at room temperature for 30 minutes. Cells were then recovered by centrifugation in a microcentrifuge for 10 minutes and washed twice with 2 ml of saline. Final resuspension was done with 400 μl saline and then spread on two agar plates. Plates were incubated at 30°C for four days.
Growth in liquid media
Inocula were prepared by resuspending bacterial biomass from MM9-succinate-agar plates into a saline solution (0.85% NaCl) to obtain an optical density (OD600) of 0.8. Test tubes containing 5-ml liquid media made of MM9-succinate with/without 0.1% proline and/or 0.1% uracil where inoculated with the inoculum at a 10% concentration. Test tubes were incubated at 30°C with constant shaking. Growth was monitored by reading the absorbance at 600 nm. Growth rate constants (μ) were calculated based on absorbance values during the exponential growth phase and using the formula: μ = ( (log10 N - log10 N0) 2.303) / (t - t0). Results represent the average of duplicates and the standard deviation was calculated as the error.
To measure transcription from reporter gene fusion strains, the β-glucuronidase assay described in Cowie et al.  was adapted. Strains were grown in MM9-succinate plus 0.1% proline, 0.1% uracil, and gentamicin until OD600 of 0.2 - 0.8. These cells were then used directly for the assay in microplates as described previously . Assays were done in triplicate and standard deviation calculated.
This research was supported by a Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant to T.C.C. L.B. received a scholarship from “Fonds québécois de la recherche sur la nature et les technologies” (FQRNT).
We thank Professor Bi-Cheng Wang and Dr. Hao Xu at University of Georgia (USA) for provision of the purified ChvI protein and Professor Turlough M. Finan from McMaster University (Canada) who made the fusion library available to us. We are grateful to Jennifer Moore and Jacquelyn Fleming for technical assistance, Dr. Jiujun Cheng for critically reading the manuscript, and Kathy Lam and John Heil for assistance with data analysis.
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