Antifeedant, larvicidal and growth inhibitory bioactivities of novel polyketide metabolite isolated from Streptomyces sp. AP-123 against Helicoverpa armigera and Spodoptera litura
© Arasu et al.; licensee BioMed Central Ltd. 2013
Received: 21 November 2012
Accepted: 30 April 2013
Published: 13 May 2013
Considerable attention has been paid to actinomycetes, especially the secondary metabolites obtained from Streptomyces species, as the best alternatives to chemicals as biological control agents for polyphagous pests such as Helicoverpa armigera and Spodoptera litura. On the basis of their novel biocontrol attributes, novel polyketide metabolite isolated from marine Streptomyces sp. AP-123 exhibited significant antifeedant, larvicidal and growth inhibitory activities against polyphagous pests.
Leaf disc no-choice method was used for the insect bioassay. The polyketide metabolite presented significant antifeedant activities against H. armigera (78.51%) and S. litura (70.75%) at 1000 ppm concentration. The metabolite also exhibited high larvicidal activities against H. armigera (63.11%) and S. litura (58.22%) and the LC50 values were 645.25 ppm for H. armigera and 806.54 ppm for S. litura. The metabolite also prolonged the larval–pupal duration of the insects at all the tested concentrations.
The activities of the polyketide metabolite were concentration dependent for both the insects therefore it could be used as an agent to prepare new pesticidal formulations.
KeywordsStreptomyces sp. AP-123 Polyketide metabolite Antifeedant Larvicidal Helicoverpa armigera Spodoptera litura
According to the report of FAO, US $120 billion losses worldwide were caused by 20–40% decrease in crop yield, due to the attack from pathogenic organisms and insect pests . Helicoverpa armigera and Spodoptera litura are the major polyphagous pests attacking more than 150 different host species and affect the vegetable yield . Therefore these pests are considered as the most economically important insect pests in many countries including India, Japan, China and Southeast Asia. Controlling these polyphagous pests becomes the challenging work in agriculture field. There are few chemical insecticides and pesticides are commercially available in the market. Usage of different varieties of chemical insecticides and pesticides to control insects has resulted in emergence of pesticide resistance in the pests . To ensure the stable and high output of crops, huge amount of pesticides were applied to control the pests, and this not only caused serious environmental pollution but also induced in a wide range of pesticide resistance. Meanwhile by applying these chemical pesticides different varieties of pest predators were killed and the ecological balance was destroyed, thereby causing pest resurgence and a greater outbreak of secondary pests . Due to this reason, many researchers have involved on alternative control methods. Botanical and microbial pesticides are having advantage over chemical pesticides by its highly effective, safe, and ecologically acceptable nature. Fortunately, bio-pesticides have been gaining increased attention and interest among those concerned with developing environment friendly and safe integrated crop management, with compatible approaches and tactics for pest management .
Natural products derived from plants and microorganisms have been used for insect control . Azadirachtin, a natural compound isolated from neem Azadirachta indica, is considered superior over other compounds since it has wide range of biological activities. Azadirachtin has been studied by many researchers and used as positive control. Bacterial and viral-based insecticides controlled different pests. Most of the pesticides from microorganisms have been isolated from entomo-pathogens and the terrestrial environment . Recent studies on marine microorganisms have focused mainly on the discovery of human drugs, whereas limited information about marine microorganisms possessing insecticidal activities has been reported. However marine environment, representing more than two thirds of our planet, is still under-explored and is considered to be a prolific resource for the isolation of less exploited microorganisms . The ocean is a resource of huge drug, where more than 6000 kinds of novel chemical compounds have been isolated from marine living organisms, among which more than 1000 compounds exert biological activities, such as anti-tumour, anti-microbial and anti-virus, etc. .
Results and discussion
Antifeedant activity of polyketide metabolite against H. armigera and S. litura
Antifeedant activity (%)
38.01 ± 2.11b
35.93 ± 3.14b
51.77 ± 3.81c
46.19 ± 3.88c
64.29 ± 3.78d
59.58 ± 2.41d
78.51 ± 3.90e,f
70.75 ± 2.46e,f
62.20 ± 3.05d
65.47 ± 2.92e
64.37 ± 3.26d,e
75.41 ± 5.34f
74.51 ± 4.95f
83.73 ± 3.53g
89.84 ± 5.65g
89.61 ± 2.88h
4.33 ± 1.07a
1.54 ± 1.04a
Larvicidal(%) and effective concentrations (LC 50 and LC 90 ppm) of polyketide metabolite against H. armigera and S. litura
15.52 ± 5.29a
10.44 ± 0.60a
33.16 ± 4.34b
29.11 ± 4.11b
54.08 ± 5.63c
47.77 ± 3.04c
68.41 ± 6.04d
60.02 ± 2.43d
47.77 ± 4.26c
51.98 ± 5.95c,d
63.66 ± 4.47d
69.18 ± 6.42e
98.77 ± 4.45e
95.77 ± 5.18f
100 ± 00e
100 ± 00f
Growth inhibitory activity of polyketide metabolite against H. armigera and S. litura
Larval duration (d)
Pupal duration (d)
Larval duration (d)
Pupal duration (d)
10.09 ± 0.44b
20.99 ± 4.15b
11.45 ± 0.40b
10.02 ± 0.29a,b
18.51 ± 6.33b
10.28 ± 0.22a
10.91 ± 0.35b,c
32.58 ± 5.20b,c
12.35 ± 0.46b,c
10.44 ± 0.87b
25.06 ± 7.22b
11.53 ± 0.69b
12.55 ± 0.37c
42.55 ± 3.47c
13.50 ± 0.70c
11.96 ± 0.45c
47.13 ± 10.9c
13.86 ± 0.63c
13.98 ± 0.51d
62.01 ± 11.7d
15.45 ± 1.03d
13.96 ± 0.92c
55.06 ± 9.12c
14.4 ± 0.54cd
14.09 ± 0.16e
70.45 ± 9.04d
17.95 ± 0.54e
14.56 ± 0.26d,e
47.40 ± 7.48c
14.10 ± 0.48c
15.8 ± 0.74f
100 ± 00e
15.95 ± 0.98e
76.08 ± 12.9d
15.24 ± 0.5d
9.08 ± 0.15a
8.95 ± 0.49a
11.12 ± 0.39a
In the present study, polyketide metabolite exhibited maximum antifeedant activity of 78.51% and 70.75% at 1000 ppm concentration against H. armigera and S. litura. This result coincided with earlier results of Kannan who had isolated violacein from Chromobacterium violaceum claimed more than 80% antifeedancy at 1000 ppm against H.armigera . Xiang et al. isolated novel macrocyclic lactone from Streptomyces microflavus neau3, showed high acaricidal activity against adult mites and nematocidal activity against Caenorhabditis elegans .
In the present study, significant larvicidal activity was observed at 1000 ppm concentration against H. armigera and S. litura, respectively. Becher et al. reported that 12-epi-Hapalindole J isonitrile isolated from soil bacterium showed 100% larvicidal activity against Chironomus riparius . Three different strains of B. thuringiensis showed larvicidal activity ranging between 62% and 96% against Spodoptera frugiperda and 100% against Anticarsia gemmatalis . In this study some adults emerged and were small in size with varied abnormalities. 20% of the world’s top-selling pharmaceuticals and their and derived compounds were polyketide in nature and thus represent one of the most important classes of natural products, with combined revenues of over $18 billion per year . Microbial polyketides are synthesized by serialized reactions of a set of enzymes called PKS with extraordinary structural diversity and an irregular distribution between strains and species, and they have been considered to play vital roles antimicrobial agents for pathogenic bacteria, fungi and also used as in pest control agents to kill insects and pests . Spinosyns recovered from microorganism showed potent insecticidal activities against many commercially significant species that cause extensive damage to crops and other plants. They also exhibit activity against important external parasites of livestock, companion animals and humans . Several microbial polyketides, such as avermectins and milbemycins, have been reported as potent insecticides against various insects and parasites. Furthermore, they are believed to be the biggest selling and arguably most effective acaricides and anthelmintics currently available . Of the 7000 known polyketide structures, more than 0.3% has been commercialized . Given the importance and potential of these compounds, the discovery of microbial polyketides has drawn increasing attention.
In conclusion, polyketide metabolite showed good antifeedant, larvicidal, pupicidal and growth inhibitory activities against H. armigera and S. litura. The results indicated that polyketide metabolite would be a potential insecticide. This study is the first report on antifeedant, larvicidal, pupicidal and growth inhibitory activities against H. armigera and S. litura. This metabolite could be used for the development of new insecticidal formulation for the management of field pests.
Isolation and identification of Streptomycessp. AP-123
Isolation and identification of polyketide metabolite
Isolation of polyketide metabolite and its identification have already been described in our earlier manuscript .
Insect culture collection and monitoring
Larvae of S. litura and H. armigera were collected from the farmers’ field in Kancheepuram district, Tamil Nadu. Insects were cultured by following the methods of Basker et al. . Briefely, the collected H. armigera larvae were reared individually in a plastic container (vials) and fed regularly with lady’s finger, Abelmoschus esculentus L. (Malvaceae) and S. litura larvae were reared on castor leaves and were kept till the larvae became pupae under the laboratory conditions (27 ± 2°C and 74 ± 5% relative humidity). The sterile soil was provided for pupation. After pupation, the pupae were collected from the soil and placed in inside the cage for emergence of adults. Cotton soaked with 10% honey solution (Dabur Honey, India) mixed with a few drops of multi-vitamins (Hi-Media, Mumbai) was provided for adult feeding to increase the fecundity. Potted cowpea plants were kept for H. armigera and groundnut plants were provided for S. litura separately inside the adult emergence cages for egg laying. After hatching, the larvae were collected from the cage and fed with standard artificial diet as recommended by Koul et al.  for H. armigera. Castor leaf was provided for S. litura.
Antifeedant activity of the polyketide metabolite
Antifeedant activity of polyketide metabolite was evaluated using leaf disc no-choice method described by Basker et al. . Briefly, fresh young cotton (H. arigera) and castor (S. litura) leaves were collected and cleaned thoroughly with water to remove the dust and other particles and then wiped with cotton to remove the moisture content, after that leaf discs of 4 cm diameter were punched using cork borer. Four different concentrations of the isolated metabolite such as 125, 250, 500 and 1000 ppm were evaluated in this study. The leaves disc were dipped into the metabolite for 15 min. Acetone (Thermo Fisher Scientific India Pvt. Ltd, Mumbai, India) was used as negative control since acetone was used to dissolve the compound and leaf discs dipped in azadirachtin (40.86% purity, obtained from EID-Parry India Ltd., Chennai) was used as positive control. In each plastic petridish (1.5 × 9 cm) wet filter paper was placed to avoid early drying of the leaf discs. Third instar larva of the respective insects was introduced into each petriplates. Progressive consumption of treated and control leaves by the larvae after 24 h was assessed using Leaf Area Meter (Delta-T Devices, Serial No. 15736 F 96, UK). Leaf area eaten by larvae in treatment was corrected from the negative control. Each concentrations were maintained as five replicates with 10 larvae per replicate (total, N = 50). The experiment was performed at laboratory conditions (27 ± 2°C) with 14:10 photoperiod and 75 ± 5% relative humidity. Antifeedant activity was calculated according to the formula of Bentley et al. .
Larvicidal activity of the polyketide metabolite
Larvicidal activity was studied using leaf disc no-choice method Basker et al. . Briefly, fresh cotton and castor leaf were obtained from the garden was used in this study. After cleaning the leaves with water leave discs were made and dipped in different concentrations of the compound and assayed as mentioned in antifeedant experiment. After 24 h the larvae were continuously maintained on the untreated fresh cotton and castor leaves for H. armigera and S. litura, respectively. Insect diet was changed every 24 h. Larval mortality was observed and recorded after 96 h of treatment. Five replicates were maintained for each treatment with 10 larvae per replicate (total N = 50). The laboratory conditions were maintained as same as in the antifeedant experiment. Percent mortality was calculated according to Abbott .
Pupicidal activity of the polyketide metabolite
The larvae which survived were continuously fed with normal diet as specified in larvicidal activity until they became pupae and adults. Pupicidal activity was calculated by subtracting the number of emerging adults from the total number of pupae.
Larval and pupal durations
The survived larvae in the treatments were reared on fresh untreated leaves and their larval duration after the treatment was recorded. Pupal period was calculated from the day of pupation to the day of adult emergence.
The data related to antifeedant, larvicidal and pupicidal activities and larval–pupal durations were analysed by one way Analysis of Variance. Significant differences between treatments were determined using Tukey’s multiple range tests (P ≤ 0.05). Probit analysis was done to calculate median lethal concentration (LC50) and LC90 using SPSS 11.5 version software package .
The authors are grateful to global Research Centre for Biotechnology, Taramani, Chennai, India, Entomology Research Institute, Loyola College and CNU for carrying out this work. Authors are thankful to Addiriyah Chair for Environmental Studies, Department of Botany and Microbiology, College of Science, King Saud University, Riyadh-11451, Saudi Arabia for financial assistance.
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