Table 2 Description of 12 VNTR loci analyzing with 202 H.pylori clinical isolates
Locus | Forward and Reverse primer (F/R) | Annealing temperature (°C) | Expected product length in 26695 (bp) | Product size range | Allele size range(unites) | Total number of alleles | Nei's diversity index |
|---|---|---|---|---|---|---|---|
VNTR-180 | F:TAAAGTGAAAGCGTTACAAAAAGAC R:CTTCAGGGTAGGAATACAGCAGAGT | 53 | 185 | 165-225 | 1-4 | 4 | 55. 7 |
VNTR-263 | F:TTGAATTGCAAGCTAATGAGTC R:AGAAGTGTTGATGCTAGAAGAG | 52 | 352 | 310-366 | 1-5 | 5 | 63. 0 |
VNTR-614 | F:ATTGATTATGATTTTCTTGGCAATTTTG R:GCTTATGAATGTGTGTTTTGCTGATGAC | 54 | 758 | 334-864 | 1-7, 11 | 9 | 80. 7 |
VNTR-557 | F:ATGGAAGTTTTTGATTTGATTG R:GGTGTAATGGGTGTTGATGGTC | 50 | 152 | 152-202 | 1-3, | 3 | 12. 3 |
VNTR-607 | F:GAATTGATTATGATTTTCTTGGCAAT R: GCTGAAAACGCTAGGGATAGAGC | 52 | 668 | 233-673 | 1, 2, 5-21, 23 | 20 | 92. 8 |
VNTR-1801 | F:GCCGTATTTTAGGATAAAGCAAAG R:CGCGTTTTATAGCGCTTCTTATT | 52 | 280 | 280-604 | 1-5, 12 | 5 | 57. 3 |
VNTR-2181 | F:TTATGGAAAATATCATACAACCCCCTAT R:ATTTAGAAAAATTACCCCTTTCATCAAG | 52 | 378 | 378-426 | 1-3, 5 | 4 | 20. 9 |
VNTR-2457 | F:TAGAAGATTGCTTGAAAAGCCCTTT R:GCTCTATGATTTTAAAACGCTCCGT | 52 | 650 | 650-812 | 1-4 | 4 | 73. 6 |
VNTR-2576 | F:GATTTTTGATARGCTTTGCGATAG R:TAAAACGATTTTAGAAAACGACAC | 51 | 371 | 182-371 | 1-7, 10 | 8 | 46. 2 |
VNTR-5062 | F:AAGCTCGCCCTCATCGCC R:TAAAAAATATTAAATAATCAATT | 50 | 307 | 223-259 | 1-4 | 4 | 40. 9 |
VNTR-5282 | F:CCTTAAGCTCTTTAGGGGCTGG R:GAGAGTTCTAGGGGCGTGGC | 56 | 335 | 335-371 | 1-4 | 4 | 36. 2 |
VNTR-5581 | F:CGTTCACTCTGAGCCAGGATC R:GCTCTTTCTGTTTTGTTGTTGTAAT | 52 | 202 | 190-218 | 1-3 | 3 | 34. 3 |