Table 1 Strains and plasmids used in this study
From: Identification and evaluation of the role of the manganese efflux protein in Deinococcus radiodurans
Strain or plasmid | Relevant marker | Reference or resource |
|---|---|---|
Strains | ||
E. coli DH5α | hsdR17 recA1 endA1 lacZΔM15 | Invitrogen |
D. radiodurans R1 | ATCC13939 | |
mntE - | As R1, but mnE::aadA | This study |
mntR | As mntE- mnE::aadA(pME mntE Dr +) | This study |
Plasmids | ||
pMD18-T | TA cloning vector | Takara |
pRADK | E. coli-D. radiodurans shuttle vector carrying D. radiodurans groEL promoter | [27] |
pME | pRADK derivative expressing D. radiodurans mntE | This study |