Table 1 The primer sequences used in this study
Target gene | Oligonucleotide sequence (5′ to 3′) | Annealing temperature (◦C) | Product size (bp) | Reference |
|---|---|---|---|---|
blaTEM | F: GAGTATTCAACATTTCCGTGTC R: TAATCAGTGAGGCACCTATCTC | 60 | 800 | 17 |
blaSHV | F: CGCCTGTGTATTATCTCCCTGTTAGCC | 60 | 843 | 17 |
| Â | R: TTGCCAGTGCTCGATCAGCG | Â | ||
blaCTX−M−14 | F: TTATGCGCAGACGAGTGCGGTG | 55 | 120 | 19 |
| Â | R: TCACCGCGATAAAGCACCTGCG | Â | ||
blaCTX−M−15 | F: GAGCCGACGTTAAACACCGCCA R: GCTGCACCGGTGGTATTGCCTT | 58 | 156 | 19 |
blaCIT | F: TGGCCAGAACTGACAGGCAAA R: TTTCTCCTGAACGTGGCTGGC | 57 | 462 | 17 |
blaMOX | F: GCTGCTCAAGGAGCACAGGAT R: CACATTGACATAGGTGTGGTGC | 57 | 520 | 17 |
blaDHA | F: AACTTTCACAGGTGTGCTGGGT | 57 | 405 | 17 |
| Â | R: CCGTACGCATACTGGCTTTGC | Â | ||
blaEBC | F: TCGGTAAAGCCGATGTTGCGG R: CTTCCACTGCGGCTGCCAGTT | 57 | 302 Variable | 17 |
ERIC | F: ATGTAAGCTCCTGGGGATTCAC R: AAGTAAGTGACTGGGGTGAGCG | 51 | 11 |