Table 3 The primers employed for identifying Cryptosporidium spp., and subtypes of C. parvum, C. viatorum, C. felis in the present study
Usage (s) | Primer sequence (5′ to 3′) | Fragment length (bp) | Annealing temperature (°C) | Gene | Ref |
|---|---|---|---|---|---|
Cryptosporidium genus specific | 18SiCF2: GACATATCATTCAAGTTTCTGAC 18SiCR2: CTGAAGGAG TAAGGAACAACC 18SiCF1: CCTATCAGCTTTAGACGGTAGG 18SiCR1: TCTAAGAATTTCACCTCTGACTG | ∼ 587 | 58 58 | SSU rRNA | [17] |
Subtyping of C. parvum | AGP-F1: ATAGTCTCCGCTGTATTC AGP-R1: GGAAGGAACGATGTATCT AGP-F2: TCCGCTGTATTCTCAGCC AGP-R2: GCAGAGGAACCAGCATC | ∼ 850 | 55 58 | gp60 | [9] |
Subtyping of C. viatorum | CviatF2: TTCATTCTGACCCCTTCATAG CviatR5: GTCTCCTGAATCTCTGCTTACTC CviatF3: GAGATTGTCACTCATCATCGTAC CviatR8: CTACACGTAAAATAATTCGCGAC | ∼ 950 | 55 52 | gp60 | [11] |
Subtyping of C. felis | GP60CF_F1: TTTCCGTTATTGTTGCAGTTGCA GP60CF_R1: ATCGGAATCCCACCATCG AAC GP60CF_F2: GGGCGTTCTGAAGGATGT AA GP60CF_R2: CGGTGGTCTCCTCAGTCTTC | ∼ 900 | 55 55 | gp60 | [10] |