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Table 2 The sequences of gene expression primers used for Real-time PCR assay

From: Neutrophil extracellular traps formation: effect of Leishmania major promastigotes and salivary gland homogenates of Phlebotomus papatasi in human neutrophil culture

Genes

Sequences of primers (5′-3′)

Ref

GAPDH

Forward: CCACTCCTCCACCTTTGACG

Reverse: CCACCACCCTGTTGCTGTAG

[29,30,31]

MMP9

Forward: GCCACTACTGTGCCTTTGAGTC

Reverse: CCCTCAGAGAATCGCCAGTACT

[32,33,34]

MPO

Forward: GAGCAGGACAAATACCGCACCA

Reverse: AGAGAAGCCGTCCTCATACTCC

[35, 36]

NE

Forward: GGAGCCCATAACCTCTCGC

Reverse: GAGCAAGTTTACGGGGTCGT

[37]