Table 1 Bacterial strains and plasmids used in this study
From: Staphylococcus aureus foldase PrsA contributes to the folding and secretion of protein A
Strains or Plasmids | Description | Reference |
|---|---|---|
Strain | Â | Â |
E. coli | Â | Â |
 EPI300 | a host for cloning | Epicentre |
 BL21(DE3) | a host for expressing recombinant proteins | [51] |
S. aureus | Â | Â |
 RN4220 | a restriction-defective strain derived from S. aureus NCTC8325-4 was used as a host for cloning | [52] |
 HG001 | a derivative of S. aureus NCTC8325 | [53] |
 HG001ΔprsA | an isogenic mutant derived from strain HG001, containing a deletion in prsA; prsA::spec | [19] |
 HG001ΔprsA (pGHL-PrsA) | HG001ΔprsA complemented with pGHL-PrsA; restored for PrsA production | [19] |
 SA113Δspa | an isogenic mutant derived from strain SA113, containing a deletion in spa; spa::erm | [53] |
Plasmid | Â | Â |
 pGHL7 | a shuttle vector carrying a pC194 ori, a ColE1 ori, an Ap resistance gene, and a Cm resistance gene, was used for cloning | [19] |
 pGHL-PrsA | a derivative of pGHL7, containing prsA | [19] |
 pGHL-SpA-His | a derivative of pGHL7, containing a DNA fragment that encodes the full-length protein A with a C-terminal His-tag | This study |
 pET-PrsA | a derivative of pET-30b, containing a DNA fragment that encodes the full-length PrsA (amino acid 1 to 320) | [19] |
 pET-PrsA-D1 | a derivative of pET-PrsA, containing a DNA fragment that encodes amino acids 1 to 256 in PrsA | This study |
 pET-PrsA-D2 | a derivative of pET-PrsA, containing a DNA fragment, which encodes amino acids 1 to 146 in PrsA | This study |
 pET-PrsA-D3 | a derivative of pET-PrsA, containing a DNA fragment, which encodes amino acids 1 to 146 and 251 to 320 in PrsA | This study |
 pET-PrsA-D4 | a derivative of pET-PrsA, containing a DNA fragment, which encodes amino acids 42 to 320 in PrsA | This study |
 pET-PrsA-D5 | a derivative of pET-PrsA, containing a DNA fragment, which encodes amino acids 114 to 320 in PrsA | This study |
 pET-PrsA-D6 | a derivative of pET-PrsA, containing a DNA fragment, which encodes amino acids 243 to 320 in PrsA | This study |