Fig. 5
From: Staphylococcus aureus foldase PrsA contributes to the folding and secretion of protein A
Binding of immunoglobulin IgG to S. aureus. (A) S. aureus HG001, HG001ΔprsA, and HG001ΔprsA(pGHL-PrsA) were incubated with mouse immunoglobulin IgG and FITC-conjugated anti-mouse IgG antibodies. FITC-labeled S. aureus was analyzed by flow cytometry. The white area and the blue area indicate the fluorescence intensity distributions of S. aureus strains stained with or without FITC-conjugated antibody. Unstained S. aureus (blue area) was used to set the negative population. (B) The amount of FITC-labeled S. aureus was determined by flow cytometry and expressed as the percentage bound relative to the negative population. Data are presented as the mean of the results from three independent experiments. Error bars denote the standard deviations. Significant differences are denoted *p < 0.05, ***p < 0.001