Fig. 2
From: Staphylococcus aureus foldase PrsA contributes to the folding and secretion of protein A
Stability of SpA exported to the cell wall. (A) S. aureus HG001 and HG001ΔprsA were cultured for 5 h and treated with erythromycin to inhibit protein synthesis. The cell wall-associated proteins were then extracted at the time indicated after adding erythromycin. The amount of SpA was determined by immunoblotting. (B) Bands from the experiments in panel A were quantified using a densitometer. The half-life of SpA was calculated from the analysis of the exponential regression curve using the GraphPad Prism software. The cell lysates prepared from 5 × 108 CFU of cells were separated by SDS-PAGE and electrotransferred onto PVDF membranes. The PVDF membranes were stained with 0.2% Ponceau S solution as a loading control to ensure the equivalent protein content extracted from an equal number of cells (Supplemental Fig. S2)