Fig. 1
From: Staphylococcus aureus foldase PrsA contributes to the folding and secretion of protein A
Secretion of SpA by S. aureus. S. aureus HG001 and S. aureus HG001ΔprsA were cultured in TSB for 1 h (lanes 1 and 5), 3 h (lanes 2 and 6), 5 h (lanes 3 and 7), and 7 h (lanes 4 and 8). (A) Cell wall-associated proteins were prepared from 5 × 108 CFU of cells, and SpA in the fraction was analyzed by immunoblotting using anti-SpA antibody. (B) After culturing the cells, the proteins in the culture medium were concentrated using Amicon Ultra centrifugal filters and detected by immunoblotting. The cell lysates prepared from 5 × 108 CFU of cells were separated by SDS-PAGE and electrotransferred onto PVDF membranes. The PVDF membranes were stained with 0.2% Ponceau S solution as a loading control to ensure the equivalent protein content extracted from an equal number of cells (Supplemental Fig. S1)