Table 1 Designed primers and PCR products size of enterobactin binding, efflux proteins, DNA gyrase, and MurA genes
Gene | product | PCR product size (bp) | Primer | Ta (°C) | ||
|---|---|---|---|---|---|---|
Enterochelin binding protein | FepA | siderophore enterobactin receptor FepA | 366 | 714F21 | 5' GTATGCCACGACGTTACCAGC 3' | 59.0 |
1060R20 | 5' ACGAAATCCTGTGTCGCTTT 3' | |||||
FepB | Fe2+-enterobactin ABC transporter substrate binding protein | 503 | 291F21 | 5' ACTGCAACGGCTCTATATCGG 3' | 59.2 | |
773R21 | 5' ACAGGAATAGTGACTCGCCAT 3' | |||||
FepC | iron-enterobactin ABC transporter ATP-binding protein | 451 | 313F21 | 5' GGACGTTATCCGCATCAACCG 3' | 59.0 | |
744R20 | 5' TTCCGGCAACTGGATCGTCA 3' | |||||
FepD | Fe3+ -siderophore ABC transporter permease | 481 | 434F25 | 5' AGTTAAGTCCGGTGCGTTTAACCCT 3' | 62.1 | |
894R21 | 5’ GGCACAATCACCCGCCCGATG 3’ | |||||
FepG | iron-enterobactin ABC transporter permease | 649 | 12F23 | 5’ CTCTCGCCGATTACTCATCACCT 3’ | 61.5 | |
639R22 | 5' GCCCATTTCCAGCAAGCGCATC 3' | |||||
Efflux proteins | AcrB | efflux RND transporter permease AcrB | 358 | 680F20 | 5' GCCAACAGCTTAACGCCTCT 3' | 58.6 |
1017R21 | 5' TCGACCAGCGTTTTAACCACT 3' | |||||
TolC | outer membrane channel protein TolC | 388 | 97F20 | 5' CAAGCACGCCTTAGTAACCC 3' | 56.5 | |
463R22 | 5' CTAATTGACGGTAGATCGCTTC 3' | |||||
DNA gyrase | DNA topoisomerase (ATP-hydrolyzing) subunit B | 500 | 183F23 | 5' CACCATTCACGCCGATAACTCTG 3' | 59.9 | |
661R22 | 5' ATTCAACGAACGCCTTGATGCC 3' | |||||
MurA | UDP-N acetylglucosamine 1-carboxyvinyltransferase | 710 | 393F24 | 5' ATTAGGCGCGACCATCAAACTGGA 3' | 60.7 | |
1081R22 | 5' TTGCCATAACCTGTGCGCCAGA 3' | |||||