Skip to main content

Table 2 Reactivation of UVC treated C. burnetii NMII

From: Ultraviolet C inactivation of Coxiella burnetii for production of a structurally preserved whole cell vaccine antigen

1Time after

UVC exposure (h)

cfu/ml (SD)

2C. burnetii (GE/ml) in BGM cells

undiluted

1:10

1:100

1:1000

3Dilution with CCV formation

Untreated

2.47E + 08 (9.07E + 07)

1.62E + 07 (8.95E + 06)

6.97E + 06 (4.81E + 06)

6.59E + 06 (2.87E + 06)

4.06E + 06 (2.02E + 06)

undiluted to 1E-07

1

0

6.31E + 05 (1,61E + 05)

7.10E + 04 (1.63E + 04)

9.90E + 03 (1.34E + 03)

n.d.

n.d.

24

0

4.84E + 05 (4.86E + 04)

5.05E + 04 (7.77E + 03)

1.64E + 04 (2.25E + 04)

n.d.

n.d.

48

0

5.63E + 05 (3.85E + 05)

3.14E + 04 (2.53E + 03)

3.48E + 03 (8.39E + 02)

n.d.

n.d.

  1. 1C. burnetii NMII content was determined before (untreated) and at 1 h, 24 or 48 h after UVC treatment at 100 µW/cm2 for 20 min
  2. 2Bacteria were serially diluted prior inoculation of BGM cells and bacterial load was determined by qPCR detecting viable and dead bacteria after incubation for seven days
  3. 3CCV were detected by microscopy, wells with at least two clearly visible CCVs were considered positive.n.d., not detected
Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us